Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0067 and PP_0068 are separated by 60 nucleotides PP_0068 and PP_0069 are separated by 206 nucleotides PP_0069 and PP_0070 are separated by 42 nucleotides PP_0070 and PP_5425 are separated by 133 nucleotides
PP_0067: PP_0067 - Methionyl-tRNA formyltransferase, at 77,540 to 78,472
_0067
PP_0068: PP_0068 - peptide deformylase, at 78,533 to 79,039
_0068
PP_0069: PP_0069 - putative Rossmann fold nucleotide-binding protein, at 79,246 to 80,343
_0069
PP_0070: PP_0070 - Threonylcarbamoyl-AMP synthase, at 80,386 to 80,943
_0070
PP_5425: PP_5425 - conserved protein of unknown function, at 81,077 to 81,277
_5425
Position (kb) 79
80
81 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 79.040 kb on - strand at 79.040 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.128 kb on - strand at 79.128 kb on - strand at 79.137 kb on - strand at 79.137 kb on - strand at 79.148 kb on - strand at 79.182 kb on - strand at 79.213 kb on + strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.454 kb on + strand, within PP_0069 at 79.455 kb on - strand, within PP_0069 at 79.476 kb on + strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.477 kb on - strand, within PP_0069 at 79.707 kb on + strand, within PP_0069 at 79.708 kb on - strand, within PP_0069 at 79.724 kb on + strand, within PP_0069 at 80.029 kb on + strand, within PP_0069 at 80.029 kb on + strand, within PP_0069 at 80.029 kb on + strand, within PP_0069 at 80.030 kb on - strand, within PP_0069 at 80.341 kb on + strand at 80.341 kb on + strand at 80.345 kb on + strand at 80.345 kb on + strand at 80.346 kb on - strand at 80.346 kb on - strand at 80.346 kb on - strand at 80.365 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.944 kb on + strand at 80.945 kb on - strand at 80.945 kb on - strand at 81.007 kb on + strand at 81.007 kb on + strand at 81.007 kb on + strand at 81.116 kb on + strand, within PP_5425 at 81.116 kb on + strand, within PP_5425 at 81.116 kb on + strand, within PP_5425 at 81.117 kb on - strand, within PP_5425 at 81.183 kb on - strand, within PP_5425 at 81.183 kb on - strand, within PP_5425 at 81.205 kb on + strand, within PP_5425 at 81.206 kb on - strand, within PP_5425 at 81.206 kb on - strand, within PP_5425 at 81.244 kb on - strand, within PP_5425 at 81.244 kb on - strand, within PP_5425 at 81.270 kb on + strand at 81.271 kb on - strand at 81.318 kb on + strand at 81.334 kb on + strand at 81.334 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 79,040 - -1.1 79,040 - -0.2 79,086 - -2.5 79,086 - +0.1 79,086 - +0.6 79,128 - +0.8 79,128 - +0.7 79,137 - +1.3 79,137 - -1.8 79,148 - -1.3 79,182 - -0.1 79,213 + +0.1 79,214 - +0.4 79,214 - -0.3 79,214 - -1.5 79,214 - +0.5 79,298 + -0.6 79,298 + +0.7 79,298 + +0.8 79,298 + -1.5 79,454 + PP_0069 0.19 +1.7 79,455 - PP_0069 0.19 -0.4 79,476 + PP_0069 0.21 +0.6 79,477 - PP_0069 0.21 -0.4 79,477 - PP_0069 0.21 +0.9 79,477 - PP_0069 0.21 -1.4 79,477 - PP_0069 0.21 +0.0 79,707 + PP_0069 0.42 +0.6 79,708 - PP_0069 0.42 -0.0 79,724 + PP_0069 0.44 -0.6 80,029 + PP_0069 0.71 +0.3 80,029 + PP_0069 0.71 +2.8 80,029 + PP_0069 0.71 +1.4 80,030 - PP_0069 0.71 +1.2 80,341 + +0.7 80,341 + -0.2 80,345 + -0.3 80,345 + -0.5 80,346 - +0.2 80,346 - -0.6 80,346 - +0.4 80,365 + -0.3 80,944 + -1.0 80,944 + -2.1 80,944 + -1.7 80,944 + +1.0 80,944 + -1.0 80,945 - -0.5 80,945 - -1.0 81,007 + +0.9 81,007 + -0.5 81,007 + +0.0 81,116 + PP_5425 0.19 +0.8 81,116 + PP_5425 0.19 -0.8 81,116 + PP_5425 0.19 +0.5 81,117 - PP_5425 0.20 +1.0 81,183 - PP_5425 0.53 +0.6 81,183 - PP_5425 0.53 -0.9 81,205 + PP_5425 0.64 -0.9 81,206 - PP_5425 0.64 -1.1 81,206 - PP_5425 0.64 -1.0 81,244 - PP_5425 0.83 +0.2 81,244 - PP_5425 0.83 +0.2 81,270 + -1.6 81,271 - +0.4 81,318 + -1.6 81,334 + -1.3 81,334 + -0.5
Or see this region's nucleotide sequence