Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0264 and PP_0265 are separated by 42 nucleotides PP_0265 and PP_0266 are separated by 277 nucleotides
PP_0264: PP_0264 - Sensor histidine kinase, at 317,927 to 319,741
_0264
PP_0265: PP_0265 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 319,784 to 320,329
_0265
PP_0266: PP_0266 - Agmatine deiminase, at 320,607 to 321,713
_0266
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 318.816 kb on + strand, within PP_0264 at 318.816 kb on + strand, within PP_0264 at 318.816 kb on + strand, within PP_0264 at 318.817 kb on - strand, within PP_0264 at 318.817 kb on - strand, within PP_0264 at 318.860 kb on + strand, within PP_0264 at 318.861 kb on - strand, within PP_0264 at 318.890 kb on + strand, within PP_0264 at 319.191 kb on - strand, within PP_0264 at 319.324 kb on - strand, within PP_0264 at 319.324 kb on - strand, within PP_0264 at 319.413 kb on + strand, within PP_0264 at 319.413 kb on + strand, within PP_0264 at 319.414 kb on - strand, within PP_0264 at 319.414 kb on - strand, within PP_0264 at 319.414 kb on - strand, within PP_0264 at 319.584 kb on + strand at 319.584 kb on + strand at 319.584 kb on + strand at 319.584 kb on + strand at 319.585 kb on - strand at 319.585 kb on - strand at 319.585 kb on - strand at 319.585 kb on - strand at 319.629 kb on + strand at 319.629 kb on + strand at 319.629 kb on + strand at 319.632 kb on + strand at 319.632 kb on + strand at 319.632 kb on + strand at 319.633 kb on - strand at 319.633 kb on - strand at 319.662 kb on - strand at 319.662 kb on - strand at 319.662 kb on - strand at 319.664 kb on + strand at 319.664 kb on + strand at 319.664 kb on + strand at 319.664 kb on + strand at 319.665 kb on - strand at 319.774 kb on + strand at 319.775 kb on - strand at 319.775 kb on - strand at 319.775 kb on - strand at 319.781 kb on + strand at 319.781 kb on + strand at 319.782 kb on - strand at 319.782 kb on - strand at 319.786 kb on - strand at 319.786 kb on - strand at 319.897 kb on + strand, within PP_0265 at 319.898 kb on - strand, within PP_0265 at 319.920 kb on + strand, within PP_0265 at 319.920 kb on + strand, within PP_0265 at 319.920 kb on + strand, within PP_0265 at 319.923 kb on + strand, within PP_0265 at 319.924 kb on - strand, within PP_0265 at 319.924 kb on - strand, within PP_0265 at 319.938 kb on + strand, within PP_0265 at 320.143 kb on - strand, within PP_0265 at 320.297 kb on + strand at 320.298 kb on - strand at 320.298 kb on - strand at 320.298 kb on - strand at 320.298 kb on - strand at 320.314 kb on + strand at 320.356 kb on + strand at 320.357 kb on - strand at 320.357 kb on - strand at 320.357 kb on - strand at 320.357 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.395 kb on - strand at 320.412 kb on + strand at 320.412 kb on + strand at 320.412 kb on + strand at 320.412 kb on + strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.423 kb on + strand at 320.423 kb on + strand at 320.424 kb on - strand at 320.424 kb on - strand at 320.428 kb on + strand at 320.681 kb on - strand at 320.681 kb on - strand at 320.681 kb on - strand at 320.749 kb on + strand, within PP_0266 at 320.900 kb on + strand, within PP_0266 at 320.900 kb on + strand, within PP_0266 at 320.938 kb on + strand, within PP_0266 at 320.938 kb on + strand, within PP_0266 at 320.938 kb on + strand, within PP_0266 at 320.939 kb on - strand, within PP_0266 at 320.982 kb on + strand, within PP_0266 at 320.982 kb on + strand, within PP_0266 at 320.982 kb on + strand, within PP_0266 at 321.091 kb on + strand, within PP_0266 at 321.091 kb on + strand, within PP_0266 at 321.092 kb on - strand at 321.214 kb on + strand, within PP_0266 at 321.214 kb on + strand, within PP_0266 at 321.215 kb on - strand, within PP_0266 at 321.234 kb on + strand, within PP_0266 at 321.234 kb on + strand, within PP_0266 at 321.235 kb on - strand, within PP_0266 at 321.276 kb on + strand, within PP_0266 at 321.276 kb on + strand, within PP_0266 at 321.299 kb on - strand, within PP_0266 at 321.299 kb on - strand, within PP_0266 at 321.299 kb on - strand, within PP_0266
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 318,816 + PP_0264 0.49 +1.4 318,816 + PP_0264 0.49 +0.7 318,816 + PP_0264 0.49 +1.5 318,817 - PP_0264 0.49 -0.6 318,817 - PP_0264 0.49 -0.0 318,860 + PP_0264 0.51 -0.3 318,861 - PP_0264 0.51 -1.3 318,890 + PP_0264 0.53 -0.2 319,191 - PP_0264 0.70 +0.4 319,324 - PP_0264 0.77 -0.6 319,324 - PP_0264 0.77 +0.7 319,413 + PP_0264 0.82 +0.6 319,413 + PP_0264 0.82 -0.9 319,414 - PP_0264 0.82 -0.6 319,414 - PP_0264 0.82 +0.1 319,414 - PP_0264 0.82 -0.6 319,584 + +0.1 319,584 + +1.6 319,584 + -1.2 319,584 + -0.7 319,585 - +1.1 319,585 - -0.7 319,585 - -0.4 319,585 - -0.1 319,629 + +0.2 319,629 + -0.1 319,629 + -0.6 319,632 + +0.4 319,632 + -2.0 319,632 + +0.6 319,633 - -0.2 319,633 - -1.8 319,662 - +0.1 319,662 - +0.1 319,662 - +0.3 319,664 + +0.5 319,664 + +1.1 319,664 + +0.8 319,664 + -1.4 319,665 - +0.6 319,774 + -0.9 319,775 - -0.1 319,775 - +1.1 319,775 - -1.7 319,781 + +0.7 319,781 + -2.9 319,782 - +0.6 319,782 - +0.7 319,786 - +1.6 319,786 - -0.2 319,897 + PP_0265 0.21 +1.1 319,898 - PP_0265 0.21 -2.0 319,920 + PP_0265 0.25 +0.6 319,920 + PP_0265 0.25 -1.3 319,920 + PP_0265 0.25 -0.6 319,923 + PP_0265 0.25 +1.8 319,924 - PP_0265 0.26 +1.3 319,924 - PP_0265 0.26 +0.7 319,938 + PP_0265 0.28 -1.7 320,143 - PP_0265 0.66 +0.5 320,297 + +0.8 320,298 - +0.1 320,298 - -0.6 320,298 - +0.5 320,298 - +0.6 320,314 + +0.1 320,356 + -0.2 320,357 - -0.8 320,357 - -0.7 320,357 - -0.9 320,357 - -0.0 320,366 - -0.4 320,366 - -0.4 320,395 - +0.0 320,412 + -2.5 320,412 + -0.4 320,412 + +1.9 320,412 + -0.0 320,413 - -1.4 320,413 - -1.7 320,413 - +0.7 320,413 - -0.6 320,413 - -0.1 320,423 + -0.4 320,423 + +0.2 320,424 - +0.2 320,424 - -1.0 320,428 + +1.7 320,681 - -0.5 320,681 - +0.3 320,681 - -1.5 320,749 + PP_0266 0.13 +0.3 320,900 + PP_0266 0.26 +0.4 320,900 + PP_0266 0.26 -1.3 320,938 + PP_0266 0.30 -0.3 320,938 + PP_0266 0.30 -1.7 320,938 + PP_0266 0.30 -1.3 320,939 - PP_0266 0.30 +1.6 320,982 + PP_0266 0.34 -1.8 320,982 + PP_0266 0.34 -0.7 320,982 + PP_0266 0.34 +0.2 321,091 + PP_0266 0.44 -0.4 321,091 + PP_0266 0.44 -2.0 321,092 - +2.2 321,214 + PP_0266 0.55 +0.7 321,214 + PP_0266 0.55 +1.2 321,215 - PP_0266 0.55 -1.9 321,234 + PP_0266 0.57 -0.8 321,234 + PP_0266 0.57 -0.1 321,235 - PP_0266 0.57 +0.8 321,276 + PP_0266 0.60 -0.8 321,276 + PP_0266 0.60 -0.8 321,299 - PP_0266 0.63 -0.0 321,299 - PP_0266 0.63 +0.8 321,299 - PP_0266 0.63 +1.7
Or see this region's nucleotide sequence