Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0385 and PP_5445 are separated by 343 nucleotides PP_5445 and PP_t71 are separated by 52 nucleotides PP_t71 and PP_0386 are separated by 16 nucleotides
PP_0385: PP_0385 - putative serine acetyltransferase, at 465,999 to 466,553
_0385
PP_5445: PP_5445 - conserved protein of unknown function, at 466,897 to 467,067
_5445
PP_t71: PP_t71 - tRNA-Met, at 467,120 to 467,196
_t71
PP_0386: PP_0386 - Sensory box protein, at 467,213 to 470,956
_0386
Position (kb) 467
468 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 466.149 kb on + strand, within PP_0385 at 466.149 kb on + strand, within PP_0385 at 466.218 kb on + strand, within PP_0385 at 466.299 kb on + strand, within PP_0385 at 466.300 kb on - strand, within PP_0385 at 466.374 kb on + strand, within PP_0385 at 466.375 kb on - strand, within PP_0385 at 466.456 kb on + strand, within PP_0385 at 466.457 kb on - strand, within PP_0385 at 466.512 kb on + strand at 466.564 kb on + strand at 466.565 kb on - strand at 466.565 kb on - strand at 466.616 kb on + strand at 466.616 kb on + strand at 466.688 kb on - strand at 466.728 kb on - strand at 466.756 kb on + strand at 466.865 kb on + strand at 466.865 kb on + strand at 466.903 kb on + strand at 466.904 kb on - strand at 466.904 kb on - strand at 466.922 kb on + strand, within PP_5445 at 466.923 kb on - strand, within PP_5445 at 467.006 kb on - strand, within PP_5445 at 467.051 kb on + strand at 467.051 kb on + strand at 467.052 kb on - strand at 467.070 kb on - strand at 467.202 kb on - strand at 467.202 kb on - strand at 467.215 kb on - strand at 467.215 kb on - strand at 467.217 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.520 kb on + strand at 467.521 kb on - strand at 467.521 kb on - strand at 467.567 kb on + strand at 467.589 kb on + strand, within PP_0386 at 467.589 kb on + strand, within PP_0386 at 467.590 kb on - strand, within PP_0386 at 467.766 kb on + strand, within PP_0386 at 467.766 kb on + strand, within PP_0386 at 467.767 kb on - strand, within PP_0386 at 467.810 kb on + strand, within PP_0386 at 468.093 kb on + strand, within PP_0386 at 468.094 kb on - strand, within PP_0386 at 468.094 kb on - strand, within PP_0386
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 466,149 + PP_0385 0.27 +0.2 466,149 + PP_0385 0.27 +0.4 466,218 + PP_0385 0.39 -1.4 466,299 + PP_0385 0.54 -1.0 466,300 - PP_0385 0.54 +1.4 466,374 + PP_0385 0.68 -1.7 466,375 - PP_0385 0.68 -1.2 466,456 + PP_0385 0.82 +1.0 466,457 - PP_0385 0.83 -0.2 466,512 + +0.6 466,564 + -0.9 466,565 - +0.5 466,565 - +0.8 466,616 + +0.5 466,616 + -0.2 466,688 - +0.7 466,728 - +0.2 466,756 + -2.0 466,865 + -0.0 466,865 + +1.0 466,903 + +0.9 466,904 - -0.0 466,904 - -0.7 466,922 + PP_5445 0.15 -1.4 466,923 - PP_5445 0.15 -0.9 467,006 - PP_5445 0.64 -0.1 467,051 + +0.7 467,051 + +0.3 467,052 - +0.5 467,070 - -0.8 467,202 - -1.4 467,202 - -0.2 467,215 - -0.9 467,215 - -0.4 467,217 - -2.3 467,262 - +2.8 467,262 - -1.4 467,262 - +0.4 467,317 - -1.2 467,317 - +0.3 467,317 - +1.0 467,520 + -2.6 467,521 - -0.7 467,521 - +1.2 467,567 + +0.5 467,589 + PP_0386 0.10 +0.7 467,589 + PP_0386 0.10 -0.0 467,590 - PP_0386 0.10 -0.8 467,766 + PP_0386 0.15 -2.4 467,766 + PP_0386 0.15 -0.2 467,767 - PP_0386 0.15 -0.8 467,810 + PP_0386 0.16 -0.8 468,093 + PP_0386 0.24 +0.6 468,094 - PP_0386 0.24 +0.5 468,094 - PP_0386 0.24 +0.8
Or see this region's nucleotide sequence