Experiment: Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1225 and PP_1226 are separated by 17 nucleotides PP_1226 and PP_t13 are separated by 60 nucleotides PP_t13 and PP_1227 are separated by 93 nucleotides
PP_1225: PP_1225 - 7-carboxy-7-deazaguanine synthase, at 1,401,006 to 1,401,653
_1225
PP_1226: PP_1226 - pre-queuosine 0 synthase, at 1,401,671 to 1,402,345
_1226
PP_t13: PP_t13 - tRNA-Lys, at 1,402,406 to 1,402,481
_t13
PP_1227: PP_1227 - putative Membrane protein, at 1,402,575 to 1,403,534
_1227
Position (kb)
1402
1403 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1401.495 kb on + strand, within PP_1225 at 1401.496 kb on - strand, within PP_1225 at 1401.496 kb on - strand, within PP_1225 at 1401.598 kb on + strand at 1401.599 kb on - strand at 1401.666 kb on + strand at 1401.666 kb on + strand at 1401.666 kb on + strand at 1401.666 kb on + strand at 1401.666 kb on + strand at 1401.667 kb on - strand at 1401.667 kb on - strand at 1401.690 kb on + strand at 1401.691 kb on - strand at 1401.691 kb on - strand at 1401.761 kb on + strand, within PP_1226 at 1401.950 kb on + strand, within PP_1226 at 1401.950 kb on + strand, within PP_1226 at 1401.950 kb on + strand, within PP_1226 at 1401.950 kb on + strand, within PP_1226 at 1401.951 kb on - strand, within PP_1226 at 1401.951 kb on - strand, within PP_1226 at 1402.005 kb on + strand, within PP_1226 at 1402.050 kb on - strand, within PP_1226 at 1402.128 kb on + strand, within PP_1226 at 1402.128 kb on + strand, within PP_1226 at 1402.129 kb on - strand, within PP_1226 at 1402.129 kb on - strand, within PP_1226 at 1402.217 kb on + strand, within PP_1226 at 1402.238 kb on + strand, within PP_1226 at 1402.338 kb on - strand at 1402.414 kb on - strand, within PP_t13 at 1402.426 kb on - strand, within PP_t13 at 1402.442 kb on - strand, within PP_t13 at 1402.468 kb on + strand, within PP_t13 at 1402.489 kb on + strand at 1402.490 kb on - strand at 1402.576 kb on + strand at 1402.759 kb on + strand, within PP_1227 at 1402.760 kb on - strand, within PP_1227 at 1402.769 kb on - strand, within PP_1227 at 1402.815 kb on + strand, within PP_1227 at 1402.815 kb on + strand, within PP_1227 at 1402.815 kb on + strand, within PP_1227 at 1402.816 kb on - strand, within PP_1227 at 1402.816 kb on - strand, within PP_1227 at 1402.934 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.936 kb on + strand, within PP_1227 at 1402.937 kb on - strand, within PP_1227 at 1402.937 kb on - strand, within PP_1227 at 1402.937 kb on - strand, within PP_1227 at 1403.203 kb on + strand, within PP_1227 at 1403.204 kb on - strand, within PP_1227 at 1403.204 kb on - strand, within PP_1227 at 1403.204 kb on - strand, within PP_1227 at 1403.244 kb on + strand, within PP_1227 at 1403.244 kb on + strand, within PP_1227 at 1403.385 kb on + strand, within PP_1227 at 1403.385 kb on + strand, within PP_1227 at 1403.478 kb on + strand at 1403.479 kb on - strand at 1403.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X remove 1,401,495 + PP_1225 0.75 +0.9 1,401,496 - PP_1225 0.76 +0.9 1,401,496 - PP_1225 0.76 -0.1 1,401,598 + -0.1 1,401,599 - -3.1 1,401,666 + +0.4 1,401,666 + +0.0 1,401,666 + -0.4 1,401,666 + -1.3 1,401,666 + -0.3 1,401,667 - +2.5 1,401,667 - -0.5 1,401,690 + +0.9 1,401,691 - -3.7 1,401,691 - -0.9 1,401,761 + PP_1226 0.13 -0.2 1,401,950 + PP_1226 0.41 +0.1 1,401,950 + PP_1226 0.41 +0.5 1,401,950 + PP_1226 0.41 +0.7 1,401,950 + PP_1226 0.41 +0.0 1,401,951 - PP_1226 0.41 -1.5 1,401,951 - PP_1226 0.41 -0.6 1,402,005 + PP_1226 0.49 +0.0 1,402,050 - PP_1226 0.56 -0.7 1,402,128 + PP_1226 0.68 +0.6 1,402,128 + PP_1226 0.68 -0.6 1,402,129 - PP_1226 0.68 +0.4 1,402,129 - PP_1226 0.68 -2.4 1,402,217 + PP_1226 0.81 -1.7 1,402,238 + PP_1226 0.84 -0.7 1,402,338 - +0.2 1,402,414 - PP_t13 0.11 -0.4 1,402,426 - PP_t13 0.26 -0.8 1,402,442 - PP_t13 0.47 -0.5 1,402,468 + PP_t13 0.82 -1.4 1,402,489 + +0.5 1,402,490 - -1.5 1,402,576 + +0.4 1,402,759 + PP_1227 0.19 +0.8 1,402,760 - PP_1227 0.19 +0.5 1,402,769 - PP_1227 0.20 -0.1 1,402,815 + PP_1227 0.25 -0.1 1,402,815 + PP_1227 0.25 +0.1 1,402,815 + PP_1227 0.25 +1.0 1,402,816 - PP_1227 0.25 +1.0 1,402,816 - PP_1227 0.25 -0.3 1,402,934 + PP_1227 0.37 -0.1 1,402,936 + PP_1227 0.38 +0.5 1,402,936 + PP_1227 0.38 -0.2 1,402,936 + PP_1227 0.38 -0.3 1,402,936 + PP_1227 0.38 +0.7 1,402,936 + PP_1227 0.38 +0.3 1,402,936 + PP_1227 0.38 +0.9 1,402,936 + PP_1227 0.38 -0.0 1,402,936 + PP_1227 0.38 -0.1 1,402,937 - PP_1227 0.38 -0.3 1,402,937 - PP_1227 0.38 -0.7 1,402,937 - PP_1227 0.38 +0.1 1,403,203 + PP_1227 0.65 -1.2 1,403,204 - PP_1227 0.66 -0.0 1,403,204 - PP_1227 0.66 -1.3 1,403,204 - PP_1227 0.66 -1.1 1,403,244 + PP_1227 0.70 -0.6 1,403,244 + PP_1227 0.70 +0.3 1,403,385 + PP_1227 0.84 +0.8 1,403,385 + PP_1227 0.84 -0.1 1,403,478 + -2.2 1,403,479 - -0.3 1,403,479 - -0.5
Or see this region's nucleotide sequence