Experiment: Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0125 and PP_0126 are separated by 31 nucleotides PP_0126 and PP_0127 are separated by 151 nucleotides PP_0127 and PP_0128 are separated by 12 nucleotides PP_0128 and PP_0129 are separated by 27 nucleotides
PP_0125: PP_0125 - putative cytochrome c-type protein, at 131,475 to 131,777
_0125
PP_0126: PP_0126 - Cytochrome c4, at 131,809 to 132,426
_0126
PP_0127: PP_0127 - Thiol:disulfide interchange protein, at 132,578 to 133,210
_0127
PP_0128: PP_0128 - conserved protein of unknown function, at 133,223 to 134,077
_0128
PP_0129: PP_0129 - GGDEF domain protein, at 134,105 to 136,042
_0129
Position (kb) 132
133
134 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 131.656 kb on + strand, within PP_0125 at 131.772 kb on - strand at 131.775 kb on + strand at 131.775 kb on + strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.790 kb on - strand at 131.803 kb on + strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.820 kb on - strand at 131.820 kb on - strand at 131.877 kb on + strand, within PP_0126 at 131.877 kb on + strand, within PP_0126 at 131.995 kb on + strand, within PP_0126 at 132.019 kb on - strand, within PP_0126 at 132.036 kb on + strand, within PP_0126 at 132.037 kb on - strand, within PP_0126 at 132.103 kb on + strand, within PP_0126 at 132.286 kb on + strand, within PP_0126 at 132.406 kb on + strand at 132.406 kb on + strand at 132.407 kb on - strand at 132.424 kb on + strand at 132.443 kb on + strand at 132.443 kb on + strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.573 kb on - strand at 132.583 kb on + strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.671 kb on + strand, within PP_0127 at 132.671 kb on + strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.740 kb on + strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.096 kb on - strand, within PP_0127 at 133.122 kb on - strand, within PP_0127 at 133.208 kb on + strand at 133.208 kb on + strand at 133.208 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.241 kb on - strand at 133.387 kb on + strand, within PP_0128 at 133.387 kb on + strand, within PP_0128 at 133.388 kb on - strand, within PP_0128 at 133.511 kb on + strand, within PP_0128 at 133.512 kb on - strand, within PP_0128 at 133.562 kb on + strand, within PP_0128 at 133.562 kb on + strand, within PP_0128 at 133.563 kb on - strand, within PP_0128 at 133.720 kb on - strand, within PP_0128 at 133.775 kb on + strand, within PP_0128 at 133.775 kb on + strand, within PP_0128 at 133.775 kb on + strand, within PP_0128 at 133.776 kb on - strand, within PP_0128 at 133.777 kb on + strand, within PP_0128 at 133.778 kb on - strand, within PP_0128 at 133.800 kb on - strand, within PP_0128 at 133.942 kb on + strand, within PP_0128 at 133.942 kb on + strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 133.943 kb on - strand, within PP_0128 at 134.056 kb on + strand at 134.056 kb on + strand at 134.056 kb on + strand at 134.075 kb on + strand at 134.076 kb on - strand at 134.076 kb on - strand at 134.139 kb on - strand at 134.143 kb on + strand at 134.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X remove 131,656 + PP_0125 0.60 +0.5 131,772 - -1.3 131,775 + +0.6 131,775 + +1.2 131,776 - +0.7 131,776 - +0.9 131,776 - -1.6 131,776 - +0.6 131,789 + +0.9 131,789 + +0.7 131,789 + -2.7 131,790 - +1.8 131,803 + -1.7 131,804 - +1.3 131,804 - -0.3 131,804 - +0.4 131,804 - +0.5 131,819 + +0.8 131,819 + -0.1 131,819 + +1.9 131,820 - +1.1 131,820 - +1.4 131,877 + PP_0126 0.11 -0.0 131,877 + PP_0126 0.11 -0.3 131,995 + PP_0126 0.30 +0.9 132,019 - PP_0126 0.34 -0.9 132,036 + PP_0126 0.37 +1.4 132,037 - PP_0126 0.37 -0.2 132,103 + PP_0126 0.48 -0.4 132,286 + PP_0126 0.77 -1.9 132,406 + +0.8 132,406 + -0.1 132,407 - +0.8 132,424 + +0.4 132,443 + +0.3 132,443 + +0.2 132,444 - +0.9 132,444 - +1.3 132,444 - -0.6 132,444 - +0.1 132,502 - -1.1 132,502 - -0.3 132,502 - +0.6 132,502 - -1.2 132,572 + -0.4 132,572 + +0.1 132,572 + +0.1 132,573 - +0.5 132,583 + -2.7 132,584 - -2.0 132,584 - -4.5 132,584 - -0.2 132,671 + PP_0127 0.15 -1.2 132,671 + PP_0127 0.15 -0.6 132,672 - PP_0127 0.15 -0.5 132,672 - PP_0127 0.15 -1.4 132,672 - PP_0127 0.15 -0.8 132,740 + PP_0127 0.26 +0.7 133,080 - PP_0127 0.79 -0.1 133,080 - PP_0127 0.79 -1.5 133,096 - PP_0127 0.82 -3.6 133,122 - PP_0127 0.86 -3.1 133,208 + +0.4 133,208 + +0.4 133,208 + -1.8 133,240 + -0.1 133,240 + +1.2 133,240 + +1.0 133,240 + +0.1 133,240 + +0.8 133,241 - -1.3 133,387 + PP_0128 0.19 +0.3 133,387 + PP_0128 0.19 -0.3 133,388 - PP_0128 0.19 -0.4 133,511 + PP_0128 0.34 +1.1 133,512 - PP_0128 0.34 -0.1 133,562 + PP_0128 0.40 +1.5 133,562 + PP_0128 0.40 +0.0 133,563 - PP_0128 0.40 -0.5 133,720 - PP_0128 0.58 -0.0 133,775 + PP_0128 0.65 +0.7 133,775 + PP_0128 0.65 +0.6 133,775 + PP_0128 0.65 +1.1 133,776 - PP_0128 0.65 -0.6 133,777 + PP_0128 0.65 +1.1 133,778 - PP_0128 0.65 -1.7 133,800 - PP_0128 0.67 -0.0 133,942 + PP_0128 0.84 +1.7 133,942 + PP_0128 0.84 +0.0 133,943 - PP_0128 0.84 +1.0 133,943 - PP_0128 0.84 -0.1 133,943 - PP_0128 0.84 +0.4 133,943 - PP_0128 0.84 -1.7 134,056 + -0.8 134,056 + -0.6 134,056 + -0.4 134,075 + -0.8 134,076 - -0.6 134,076 - -0.1 134,139 - +0.4 134,143 + +0.2 134,143 + +0.3
Or see this region's nucleotide sequence