Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0184 and PP_0185 are separated by 285 nucleotides PP_0185 and PP_0186 are separated by 118 nucleotides
PP_0184: PP_0184 - Argininosuccinate lyase, at 235,428 to 236,834
_0184
PP_0185: PP_0185 - Positive alginate biosynthesis regulatory protein, at 237,120 to 237,863
_0185
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
Position (kb) 237
238 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 236.269 kb on + strand, within PP_0184 at 236.269 kb on + strand, within PP_0184 at 236.269 kb on + strand, within PP_0184 at 236.270 kb on - strand, within PP_0184 at 236.270 kb on - strand, within PP_0184 at 236.270 kb on - strand, within PP_0184 at 236.495 kb on + strand, within PP_0184 at 236.496 kb on - strand, within PP_0184 at 236.496 kb on - strand, within PP_0184 at 236.717 kb on - strand at 236.867 kb on + strand at 236.867 kb on + strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.971 kb on + strand at 237.076 kb on + strand at 237.076 kb on + strand at 237.076 kb on + strand at 237.077 kb on - strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.119 kb on + strand at 237.119 kb on + strand at 237.120 kb on - strand at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.196 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.540 kb on - strand, within PP_0185 at 237.594 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.781 kb on - strand, within PP_0185 at 237.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X remove 236,269 + PP_0184 0.60 -3.0 236,269 + PP_0184 0.60 -2.7 236,269 + PP_0184 0.60 -3.8 236,270 - PP_0184 0.60 -3.7 236,270 - PP_0184 0.60 -3.2 236,270 - PP_0184 0.60 -3.5 236,495 + PP_0184 0.76 -2.3 236,496 - PP_0184 0.76 -4.4 236,496 - PP_0184 0.76 -2.4 236,717 - -2.2 236,867 + -1.0 236,867 + -2.1 236,868 - -1.1 236,868 - -0.6 236,868 - +0.1 236,868 - -2.8 236,868 - -2.3 236,971 + +2.4 237,076 + +0.6 237,076 + +0.6 237,076 + +1.2 237,077 - -1.3 237,081 + +1.4 237,081 + +1.3 237,081 + +3.8 237,082 - -3.4 237,082 - -3.6 237,082 - -2.0 237,083 + +1.3 237,083 + +2.4 237,083 + +0.7 237,083 + +1.4 237,084 - -0.1 237,084 - -1.8 237,084 - -3.6 237,084 - -2.3 237,084 - -2.1 237,119 + -0.9 237,119 + -0.9 237,120 - -2.7 237,195 + PP_0185 0.10 -3.3 237,195 + PP_0185 0.10 -2.6 237,195 + PP_0185 0.10 -3.4 237,196 - PP_0185 0.10 -4.3 237,379 - PP_0185 0.35 -3.4 237,379 - PP_0185 0.35 -4.0 237,379 - PP_0185 0.35 -2.9 237,540 - PP_0185 0.56 -2.0 237,594 + PP_0185 0.64 -4.2 237,615 + PP_0185 0.67 -3.0 237,615 + PP_0185 0.67 -3.0 237,615 + PP_0185 0.67 -1.4 237,615 + PP_0185 0.67 -3.4 237,615 + PP_0185 0.67 -2.6 237,615 + -0.9 237,615 + PP_0185 0.67 -2.9 237,615 + PP_0185 0.67 -2.9 237,615 + PP_0185 0.67 -2.9 237,616 - PP_0185 0.67 -1.9 237,616 - PP_0185 0.67 -3.2 237,616 - PP_0185 0.67 -3.0 237,616 - PP_0185 0.67 -4.1 237,616 - PP_0185 0.67 -3.0 237,781 - PP_0185 0.89 -3.1 237,902 - -0.9
Or see this region's nucleotide sequence