Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0186 and PP_0187 overlap by 4 nucleotides PP_0187 and PP_0188 are separated by 18 nucleotides PP_0188 and PP_0189 overlap by 4 nucleotides
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687
_0187
PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833
_0188
PP_0189: PP_0189 - putative heme biosynthesis protein, at 240,830 to 242,068
_0189
Position (kb) 239
240
241 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.686 kb on - strand at 239.693 kb on + strand at 239.694 kb on - strand at 239.694 kb on - strand at 239.730 kb on + strand at 239.730 kb on + strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.762 kb on + strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.872 kb on + strand, within PP_0188 at 239.872 kb on + strand, within PP_0188 at 239.873 kb on - strand, within PP_0188 at 240.028 kb on + strand, within PP_0188 at 240.161 kb on + strand, within PP_0188 at 240.323 kb on + strand, within PP_0188 at 240.324 kb on - strand, within PP_0188 at 240.365 kb on - strand, within PP_0188 at 240.402 kb on - strand, within PP_0188 at 240.475 kb on + strand, within PP_0188 at 240.483 kb on + strand, within PP_0188 at 240.484 kb on - strand, within PP_0188 at 240.575 kb on + strand, within PP_0188 at 240.576 kb on - strand, within PP_0188 at 240.652 kb on + strand, within PP_0188 at 240.653 kb on - strand, within PP_0188 at 240.755 kb on + strand at 240.756 kb on - strand at 240.783 kb on + strand at 240.783 kb on + strand at 240.783 kb on + strand at 240.784 kb on - strand at 240.784 kb on - strand at 240.785 kb on + strand at 240.786 kb on - strand at 240.786 kb on - strand at 240.786 kb on - strand at 240.842 kb on + strand at 240.842 kb on + strand at 240.843 kb on - strand at 240.911 kb on + strand at 240.911 kb on + strand at 240.911 kb on + strand at 240.912 kb on - strand at 240.912 kb on - strand at 240.912 kb on - strand at 240.915 kb on + strand at 240.916 kb on - strand at 240.926 kb on + strand at 240.927 kb on - strand at 241.082 kb on - strand, within PP_0189 at 241.190 kb on + strand, within PP_0189 at 241.191 kb on - strand, within PP_0189 at 241.191 kb on - strand, within PP_0189 at 241.191 kb on - strand, within PP_0189 at 241.193 kb on + strand, within PP_0189 at 241.193 kb on + strand, within PP_0189 at 241.194 kb on - strand, within PP_0189 at 241.194 kb on - strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.422 kb on - strand, within PP_0189 at 241.422 kb on - strand, within PP_0189 at 241.563 kb on + strand, within PP_0189 at 241.653 kb on - strand, within PP_0189 at 241.674 kb on + strand, within PP_0189 at 241.675 kb on - strand, within PP_0189 at 241.728 kb on - strand, within PP_0189
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X remove 239,685 + +0.8 239,685 + -0.7 239,685 + +0.7 239,685 + +0.5 239,685 + +0.6 239,686 - -0.5 239,693 + +1.9 239,694 - -2.1 239,694 - +0.7 239,730 + -3.7 239,730 + -0.6 239,731 - -0.5 239,731 - -3.8 239,731 - -1.3 239,762 + -2.6 239,763 - -1.7 239,763 - -2.6 239,763 - -1.8 239,872 + PP_0188 0.15 -0.7 239,872 + PP_0188 0.15 -4.2 239,873 - PP_0188 0.15 -2.4 240,028 + PP_0188 0.29 -1.4 240,161 + PP_0188 0.40 -1.3 240,323 + PP_0188 0.55 -2.1 240,324 - PP_0188 0.55 -1.3 240,365 - PP_0188 0.58 -3.4 240,402 - PP_0188 0.62 -2.6 240,475 + PP_0188 0.68 -2.4 240,483 + PP_0188 0.69 -0.7 240,484 - PP_0188 0.69 -1.2 240,575 + PP_0188 0.77 +0.0 240,576 - PP_0188 0.77 -1.3 240,652 + PP_0188 0.84 -3.5 240,653 - PP_0188 0.84 -4.7 240,755 + -1.9 240,756 - -1.8 240,783 + -0.3 240,783 + -1.0 240,783 + -3.9 240,784 - -3.2 240,784 - -2.4 240,785 + -2.3 240,786 - -1.5 240,786 - -0.9 240,786 - -0.7 240,842 + -3.0 240,842 + +0.5 240,843 - -0.7 240,911 + -0.6 240,911 + -2.5 240,911 + -2.0 240,912 - -2.6 240,912 - -1.8 240,912 - -4.0 240,915 + -2.3 240,916 - -2.1 240,926 + -2.6 240,927 - -3.4 241,082 - PP_0189 0.20 -1.8 241,190 + PP_0189 0.29 -1.3 241,191 - PP_0189 0.29 -0.3 241,191 - PP_0189 0.29 -3.8 241,191 - PP_0189 0.29 -0.9 241,193 + PP_0189 0.29 -2.2 241,193 + PP_0189 0.29 -0.5 241,194 - PP_0189 0.29 -2.6 241,194 - PP_0189 0.29 -2.4 241,421 + PP_0189 0.48 -1.2 241,421 + PP_0189 0.48 -1.1 241,421 + PP_0189 0.48 -0.7 241,422 - PP_0189 0.48 -1.4 241,422 - PP_0189 0.48 -0.3 241,563 + PP_0189 0.59 -1.0 241,653 - PP_0189 0.66 -1.7 241,674 + PP_0189 0.68 -1.6 241,675 - PP_0189 0.68 -1.8 241,728 - PP_0189 0.72 -0.3
Or see this region's nucleotide sequence