Strain Fitness in Pseudomonas putida KT2440 around PP_4516

Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
5,128,272	+		PP_4514	0.83	+1.1
5,128,273	-		PP_4514	0.83	-0.1
5,128,398	+		PP_4514	0.89	-0.5
5,128,398	+		PP_4514	0.89	+0.2
5,128,398	+		PP_4514	0.89	+0.7
5,128,399	-		PP_4514	0.89	+0.2
5,128,399	-		PP_4514	0.89	-0.8
5,128,399	-		PP_4514	0.89	-0.5
5,128,399	-		PP_4514	0.89	+1.2
5,128,425	+				-0.5
5,128,425	+				-0.7
5,128,425	+				-1.0
5,128,449	+				+0.2
5,128,450	-				-0.7
5,128,512	+				-0.1
5,128,512	+				+0.4
5,128,513	-				-0.6
5,128,556	-				+1.2
5,128,556	-				-0.8
5,128,575	+				-1.0
5,128,608	+				-0.5
5,128,711	+		PP_4515	0.10	+1.1
5,128,712	-		PP_4515	0.10	-0.2
5,128,754	+		PP_4515	0.20	-0.2
5,128,754	+		PP_4515	0.20	+1.0
5,128,817	+		PP_4515	0.34	+1.7
5,128,817	+		PP_4515	0.34	+0.3
5,128,818	-		PP_4515	0.34	+0.4
5,128,818	-		PP_4515	0.34	+2.4
5,128,818	-		PP_4515	0.34	+1.5
5,128,818	-		PP_4515	0.34	+1.6
5,128,922	+		PP_4515	0.58	+0.8
5,128,922	+		PP_4515	0.58	+1.6
5,128,922	+		PP_4515	0.58	-1.2
5,128,923	-		PP_4515	0.58	-0.1
5,128,923	-		PP_4515	0.58	-1.4
5,128,923	-		PP_4515	0.58	+1.3
5,129,104	+				+1.7
5,129,104	+				+0.5
5,129,121	+				+2.0
5,129,121	+				+0.5
5,129,121	+				+1.7
5,129,127	+				+0.9
5,129,127	+				+0.0
5,129,128	-				-1.9
5,129,131	+				+1.9
5,129,135	-				+1.3
5,129,158	+				+0.7
5,129,193	+				+0.8
5,129,296	+				-0.2
5,129,296	+				-0.1
5,129,297	-				+0.2
5,129,297	-				-0.8
5,129,297	-				-1.4
5,129,301	+				-1.6
5,129,301	+				+1.3
5,129,301	+				+0.6
5,129,301	+				-0.4
5,129,302	-				+0.0
5,129,302	-				-0.7
5,129,471	+		PP_4516	0.10	+1.3
5,129,472	-		PP_4516	0.10	-1.7
5,129,472	-		PP_4516	0.10	+0.1
5,129,473	+		PP_4516	0.10	-0.5
5,129,473	+		PP_4516	0.10	+0.6
5,129,473	+		PP_4516	0.10	-3.4
5,129,473	+		PP_4516	0.10	+1.6
5,129,473	+		PP_4516	0.10	+0.0
5,129,473	+		PP_4516	0.10	+0.9
5,129,473	+		PP_4516	0.10	+1.1
5,129,474	-		PP_4516	0.10	-1.2
5,129,474	-		PP_4516	0.10	-0.0
5,129,474	-		PP_4516	0.10	-1.3
5,129,474	-		PP_4516	0.10	-0.1
5,129,474	-		PP_4516	0.10	+0.4
5,129,474	-		PP_4516	0.10	-1.0
5,129,474	-		PP_4516	0.10	-1.5
5,129,632	+		PP_4516	0.18	-1.9
5,129,740	+		PP_4516	0.23	+0.7
5,129,740	+		PP_4516	0.23	+1.2
5,130,136	+		PP_4516	0.41	+0.7
5,130,136	+		PP_4516	0.41	+1.1
5,130,137	-		PP_4516	0.41	+0.9
5,130,466	+		PP_4516	0.56	-0.1
5,130,467	-		PP_4516	0.56	-0.4
5,130,467	-		PP_4516	0.56	-0.0
5,130,469	+		PP_4516	0.56	-1.3
5,130,469	+		PP_4516	0.56	-0.1
5,130,469	+		PP_4516	0.56	+0.1
5,130,470	-		PP_4516	0.57	+0.2
5,130,656	+		PP_4516	0.65	-0.4
5,130,784	+		PP_4516	0.71	+0.8
5,130,785	-		PP_4516	0.71	-1.9
5,130,943	+		PP_4516	0.79	+0.4
5,130,944	-		PP_4516	0.79	-1.4
5,130,944	-		PP_4516	0.79	+1.9
5,131,069	+		PP_4516	0.84	+0.6
5,131,069	+		PP_4516	0.84	-0.6
5,131,070	-		PP_4516	0.84	+0.6
5,131,136	+		PP_4516	0.88	-0.8
5,131,216	-				-0.8
5,131,293	+				+0.3
5,131,424	-				-0.7
5,131,534	+				+0.3
5,131,535	-				+1.0
5,131,535	-				-0.2
5,131,571	+				-1.1
5,131,637	+				-0.6
5,131,637	+				-0.0
5,131,645	+				-0.5
5,131,645	+				+1.5
5,131,645	+				+0.1
5,131,645	+				+0.7
5,131,646	-				-0.3
5,131,646	-				-1.6
5,131,646	-				-1.6
5,131,646	-				-0.1
5,131,646	-				-0.1
5,131,646	-				-1.2
5,131,646	-				+1.5
5,131,646	-				-0.0
5,131,722	+		PP_4517	0.13	-0.5
5,131,723	-		PP_4517	0.13	+0.5
5,131,740	+		PP_4517	0.16	-0.8
5,131,822	-		PP_4517	0.30	-1.6
5,132,300	+		PP_4518	0.16	-0.5