Strain Fitness in Pseudomonas putida KT2440 around PP_1977

Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1976 and PP_1977 are separated by 35 nucleotidesPP_1977 and PP_t25 are separated by 280 nucleotidesPP_t25 and PP_t26 are separated by 72 nucleotidesPP_t26 and PP_t27 are separated by 51 nucleotidesPP_t27 and PP_t28 are separated by 42 nucleotidesPP_t28 and PP_t29 are separated by 61 nucleotidesPP_t29 and PP_t30 are separated by 42 nucleotides PP_1976: PP_1976 - HlyD family secretion protein, at 2,238,573 to 2,239,736 _1976 PP_1977: PP_1977 - Glutamate--tRNA ligase, at 2,239,772 to 2,241,253 _1977 PP_t25: PP_t25 - tRNA-Ala, at 2,241,534 to 2,241,609 _t25 PP_t26: PP_t26 - tRNA-Glu, at 2,241,682 to 2,241,757 _t26 PP_t27: PP_t27 - tRNA-Ala, at 2,241,809 to 2,241,884 _t27 PP_t28: PP_t28 - tRNA-Glu, at 2,241,927 to 2,242,002 _t28 PP_t29: PP_t29 - tRNA-Ala, at 2,242,064 to 2,242,139 _t29 PP_t30: PP_t30 - tRNA-Glu, at 2,242,182 to 2,242,257 _t30 Position (kb) 2239 2240 2241 2242Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2238.808 kb on + strand, within PP_1976at 2238.919 kb on + strand, within PP_1976at 2238.920 kb on - strand, within PP_1976at 2239.460 kb on + strand, within PP_1976at 2239.461 kb on - strand, within PP_1976at 2239.507 kb on + strand, within PP_1976at 2239.508 kb on - strand, within PP_1976at 2239.508 kb on - strand, within PP_1976at 2239.508 kb on - strand, within PP_1976at 2239.528 kb on + strand, within PP_1976at 2239.547 kb on - strand, within PP_1976at 2239.635 kb on + strandat 2241.268 kb on + strandat 2241.352 kb on + strandat 2241.391 kb on + strandat 2241.762 kb on + strandat 2241.778 kb on + strandat 2241.874 kb on + strand, within PP_t27at 2242.026 kb on + strandat 2242.072 kb on - strand, within PP_t29at 2242.072 kb on - strandat 2242.072 kb on - strand, within PP_t29at 2242.072 kb on - strand, within PP_t29at 2242.129 kb on + strand, within PP_t29at 2242.180 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
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2,238,808 + PP_1976 0.20 -0.5
2,238,919 + PP_1976 0.30 -0.3
2,238,920 - PP_1976 0.30 +1.3
2,239,460 + PP_1976 0.76 -0.4
2,239,461 - PP_1976 0.76 +0.3
2,239,507 + PP_1976 0.80 -0.0
2,239,508 - PP_1976 0.80 +0.4
2,239,508 - PP_1976 0.80 +0.4
2,239,508 - PP_1976 0.80 +0.0
2,239,528 + PP_1976 0.82 -0.7
2,239,547 - PP_1976 0.84 +1.9
2,239,635 + -0.2
2,241,268 + +1.0
2,241,352 + +0.8
2,241,391 + -0.6
2,241,762 + -2.9
2,241,778 + +1.1
2,241,874 + PP_t27 0.86 +0.2
2,242,026 + +1.0
2,242,072 - PP_t29 0.11 -1.1
2,242,072 - -1.5
2,242,072 - PP_t29 0.11 +1.1
2,242,072 - PP_t29 0.11 -1.3
2,242,129 + PP_t29 0.86 -0.4
2,242,180 - +2.4

Or see this region's nucleotide sequence