Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1976 and PP_1977 are separated by 35 nucleotides PP_1977 and PP_t25 are separated by 280 nucleotides PP_t25 and PP_t26 are separated by 72 nucleotides PP_t26 and PP_t27 are separated by 51 nucleotides PP_t27 and PP_t28 are separated by 42 nucleotides PP_t28 and PP_t29 are separated by 61 nucleotides PP_t29 and PP_t30 are separated by 42 nucleotides
PP_1976: PP_1976 - HlyD family secretion protein, at 2,238,573 to 2,239,736
_1976
PP_1977: PP_1977 - Glutamate--tRNA ligase, at 2,239,772 to 2,241,253
_1977
PP_t25: PP_t25 - tRNA-Ala, at 2,241,534 to 2,241,609
_t25
PP_t26: PP_t26 - tRNA-Glu, at 2,241,682 to 2,241,757
_t26
PP_t27: PP_t27 - tRNA-Ala, at 2,241,809 to 2,241,884
_t27
PP_t28: PP_t28 - tRNA-Glu, at 2,241,927 to 2,242,002
_t28
PP_t29: PP_t29 - tRNA-Ala, at 2,242,064 to 2,242,139
_t29
PP_t30: PP_t30 - tRNA-Glu, at 2,242,182 to 2,242,257
_t30
Position (kb)
2239
2240
2241
2242 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2238.808 kb on + strand, within PP_1976 at 2238.919 kb on + strand, within PP_1976 at 2238.920 kb on - strand, within PP_1976 at 2239.460 kb on + strand, within PP_1976 at 2239.461 kb on - strand, within PP_1976 at 2239.507 kb on + strand, within PP_1976 at 2239.508 kb on - strand, within PP_1976 at 2239.508 kb on - strand, within PP_1976 at 2239.508 kb on - strand, within PP_1976 at 2239.528 kb on + strand, within PP_1976 at 2239.547 kb on - strand, within PP_1976 at 2239.635 kb on + strand at 2241.268 kb on + strand at 2241.352 kb on + strand at 2241.391 kb on + strand at 2241.762 kb on + strand at 2241.778 kb on + strand at 2241.874 kb on + strand, within PP_t27 at 2242.026 kb on + strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand at 2242.072 kb on - strand, within PP_t29 at 2242.072 kb on - strand, within PP_t29 at 2242.129 kb on + strand, within PP_t29 at 2242.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X remove 2,238,808 + PP_1976 0.20 -0.5 2,238,919 + PP_1976 0.30 -0.3 2,238,920 - PP_1976 0.30 +1.3 2,239,460 + PP_1976 0.76 -0.4 2,239,461 - PP_1976 0.76 +0.3 2,239,507 + PP_1976 0.80 -0.0 2,239,508 - PP_1976 0.80 +0.4 2,239,508 - PP_1976 0.80 +0.4 2,239,508 - PP_1976 0.80 +0.0 2,239,528 + PP_1976 0.82 -0.7 2,239,547 - PP_1976 0.84 +1.9 2,239,635 + -0.2 2,241,268 + +1.0 2,241,352 + +0.8 2,241,391 + -0.6 2,241,762 + -2.9 2,241,778 + +1.1 2,241,874 + PP_t27 0.86 +0.2 2,242,026 + +1.0 2,242,072 - PP_t29 0.11 -1.1 2,242,072 - -1.5 2,242,072 - PP_t29 0.11 +1.1 2,242,072 - PP_t29 0.11 -1.3 2,242,129 + PP_t29 0.86 -0.4 2,242,180 - +2.4
Or see this region's nucleotide sequence