Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4126 and PP_4127 are separated by 10 nucleotides PP_4127 and PP_4128 are separated by 3 nucleotides PP_4128 and PP_4129 overlap by 4 nucleotides PP_4129 and PP_4130 are separated by 40 nucleotides
PP_4126: PP_4126 - NADH-quinone oxidoreductase subunit I, at 4,664,867 to 4,665,415
_4126
PP_4127: PP_4127 - NADH-quinone oxidoreductase subunit J, at 4,665,426 to 4,665,926
_4127
PP_4128: PP_4128 - NADH-quinone oxidoreductase subunit K, at 4,665,930 to 4,666,238
_4128
PP_4129: PP_4129 - NADH:ubiquinone oxidoreductase, membrane subunit L, at 4,666,235 to 4,668,088
_4129
PP_4130: PP_4130 - NADH:ubiquinone oxidoreductase, membrane subunit M, at 4,668,129 to 4,669,661
_4130
Position (kb)
4666
4667
4668
4669 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4665.266 kb on + strand, within PP_4126 at 4665.266 kb on + strand, within PP_4126 at 4665.323 kb on + strand, within PP_4126 at 4665.323 kb on + strand at 4665.324 kb on - strand, within PP_4126 at 4665.324 kb on - strand, within PP_4126 at 4665.441 kb on + strand at 4665.441 kb on + strand at 4665.484 kb on + strand, within PP_4127 at 4665.750 kb on + strand, within PP_4127 at 4665.750 kb on + strand, within PP_4127 at 4665.751 kb on - strand, within PP_4127 at 4665.924 kb on + strand at 4666.373 kb on + strand at 4666.373 kb on + strand at 4666.374 kb on - strand at 4666.374 kb on - strand at 4666.557 kb on - strand, within PP_4129 at 4666.557 kb on - strand, within PP_4129 at 4666.692 kb on - strand, within PP_4129 at 4666.712 kb on + strand, within PP_4129 at 4666.713 kb on - strand, within PP_4129 at 4666.732 kb on + strand, within PP_4129 at 4667.030 kb on + strand, within PP_4129 at 4667.031 kb on - strand, within PP_4129 at 4667.089 kb on - strand, within PP_4129 at 4667.116 kb on - strand, within PP_4129 at 4667.192 kb on + strand, within PP_4129 at 4667.193 kb on - strand, within PP_4129 at 4667.422 kb on - strand at 4667.422 kb on - strand, within PP_4129 at 4667.486 kb on + strand, within PP_4129 at 4667.486 kb on + strand, within PP_4129 at 4667.487 kb on - strand, within PP_4129 at 4667.522 kb on + strand, within PP_4129 at 4667.523 kb on - strand, within PP_4129 at 4667.901 kb on - strand at 4668.023 kb on + strand at 4668.024 kb on - strand at 4668.085 kb on - strand at 4668.085 kb on - strand at 4668.085 kb on - strand at 4668.091 kb on - strand at 4668.091 kb on - strand at 4668.091 kb on - strand at 4668.230 kb on + strand at 4668.231 kb on - strand at 4668.231 kb on - strand at 4668.282 kb on + strand at 4668.331 kb on - strand, within PP_4130 at 4668.436 kb on + strand, within PP_4130 at 4668.582 kb on + strand at 4668.583 kb on - strand, within PP_4130 at 4668.640 kb on - strand, within PP_4130 at 4668.724 kb on - strand, within PP_4130 at 4668.724 kb on - strand, within PP_4130 at 4668.795 kb on + strand, within PP_4130 at 4668.795 kb on + strand, within PP_4130 at 4668.796 kb on - strand, within PP_4130 at 4668.917 kb on + strand, within PP_4130 at 4668.918 kb on - strand, within PP_4130 at 4669.032 kb on + strand, within PP_4130 at 4669.033 kb on - strand, within PP_4130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,665,266 + PP_4126 0.73 +2.8 4,665,266 + PP_4126 0.73 +1.1 4,665,323 + PP_4126 0.83 +1.8 4,665,323 + +0.5 4,665,324 - PP_4126 0.83 +2.1 4,665,324 - PP_4126 0.83 -0.2 4,665,441 + -2.1 4,665,441 + +2.2 4,665,484 + PP_4127 0.12 -0.5 4,665,750 + PP_4127 0.65 -1.0 4,665,750 + PP_4127 0.65 +0.4 4,665,751 - PP_4127 0.65 -0.5 4,665,924 + -0.6 4,666,373 + +0.9 4,666,373 + -0.7 4,666,374 - +3.1 4,666,374 - +1.8 4,666,557 - PP_4129 0.17 +0.4 4,666,557 - PP_4129 0.17 -0.5 4,666,692 - PP_4129 0.25 +0.2 4,666,712 + PP_4129 0.26 +2.1 4,666,713 - PP_4129 0.26 -0.2 4,666,732 + PP_4129 0.27 +1.8 4,667,030 + PP_4129 0.43 -1.1 4,667,031 - PP_4129 0.43 +0.3 4,667,089 - PP_4129 0.46 +1.8 4,667,116 - PP_4129 0.48 -1.5 4,667,192 + PP_4129 0.52 -1.2 4,667,193 - PP_4129 0.52 +1.3 4,667,422 - +2.1 4,667,422 - PP_4129 0.64 -2.2 4,667,486 + PP_4129 0.67 +2.7 4,667,486 + PP_4129 0.67 +0.9 4,667,487 - PP_4129 0.68 +1.3 4,667,522 + PP_4129 0.69 +2.0 4,667,523 - PP_4129 0.69 -0.2 4,667,901 - +0.8 4,668,023 + +0.5 4,668,024 - +0.6 4,668,085 - +2.1 4,668,085 - +0.8 4,668,085 - +1.9 4,668,091 - +0.2 4,668,091 - +1.2 4,668,091 - -0.8 4,668,230 + +1.1 4,668,231 - +1.8 4,668,231 - +1.8 4,668,282 + +0.4 4,668,331 - PP_4130 0.13 +0.6 4,668,436 + PP_4130 0.20 -0.4 4,668,582 + +2.2 4,668,583 - PP_4130 0.30 -1.4 4,668,640 - PP_4130 0.33 +1.0 4,668,724 - PP_4130 0.39 +1.0 4,668,724 - PP_4130 0.39 +1.3 4,668,795 + PP_4130 0.43 +0.8 4,668,795 + PP_4130 0.43 +1.0 4,668,796 - PP_4130 0.44 +1.1 4,668,917 + PP_4130 0.51 -1.3 4,668,918 - PP_4130 0.51 +0.9 4,669,032 + PP_4130 0.59 +0.2 4,669,033 - PP_4130 0.59 +1.0
Or see this region's nucleotide sequence