Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3365 and PP_3366 are separated by 185 nucleotides PP_3366 and PP_3367 overlap by 16 nucleotides PP_3367 and PP_3368 are separated by 267 nucleotides
PP_3365: PP_3365 - Acetolactate synthase, at 3,808,055 to 3,809,698
_3365
PP_3366: PP_3366 - Transcriptional regulator, LysR family, at 3,809,884 to 3,810,765
_3366
PP_3367: PP_3367 - conserved protein of unknown function, at 3,810,750 to 3,811,409
_3367
PP_3368: PP_3368 - Major facilitator family transporter, at 3,811,677 to 3,812,861
_3368
Position (kb)
3809
3810
3811 Strain fitness (log2 ratio)
-1
0
1
2 at 3809.708 kb on + strand at 3809.709 kb on - strand at 3809.738 kb on + strand at 3809.741 kb on + strand at 3809.741 kb on + strand at 3809.811 kb on + strand at 3809.812 kb on - strand at 3809.872 kb on + strand at 3809.872 kb on + strand at 3809.873 kb on - strand at 3810.161 kb on + strand, within PP_3366 at 3810.161 kb on + strand, within PP_3366 at 3810.167 kb on + strand, within PP_3366 at 3810.167 kb on + strand, within PP_3366 at 3810.168 kb on - strand, within PP_3366 at 3810.168 kb on - strand, within PP_3366 at 3810.168 kb on - strand, within PP_3366 at 3810.171 kb on + strand, within PP_3366 at 3810.196 kb on + strand, within PP_3366 at 3810.197 kb on - strand, within PP_3366 at 3810.197 kb on - strand, within PP_3366 at 3810.197 kb on - strand, within PP_3366 at 3810.197 kb on - strand, within PP_3366 at 3810.421 kb on + strand, within PP_3366 at 3810.421 kb on + strand, within PP_3366 at 3810.422 kb on - strand, within PP_3366 at 3810.526 kb on + strand, within PP_3366 at 3810.526 kb on + strand, within PP_3366 at 3810.540 kb on - strand, within PP_3366 at 3810.701 kb on - strand at 3810.701 kb on - strand at 3810.701 kb on - strand at 3810.751 kb on + strand at 3810.851 kb on - strand, within PP_3367 at 3810.852 kb on + strand, within PP_3367 at 3810.853 kb on - strand, within PP_3367 at 3810.968 kb on - strand, within PP_3367 at 3810.970 kb on + strand, within PP_3367 at 3810.970 kb on + strand, within PP_3367 at 3810.970 kb on + strand, within PP_3367 at 3810.985 kb on - strand, within PP_3367 at 3810.985 kb on - strand, within PP_3367 at 3811.063 kb on + strand, within PP_3367 at 3811.213 kb on - strand, within PP_3367 at 3811.265 kb on + strand, within PP_3367 at 3811.400 kb on + strand at 3811.401 kb on - strand at 3811.447 kb on - strand at 3811.543 kb on + strand at 3811.543 kb on + strand at 3811.631 kb on + strand at 3811.631 kb on + strand at 3811.631 kb on + strand at 3811.632 kb on - strand at 3811.632 kb on - strand at 3811.632 kb on - strand at 3811.632 kb on - strand at 3811.632 kb on - strand at 3811.632 kb on - strand at 3811.632 kb on - strand at 3811.666 kb on + strand at 3811.667 kb on - strand at 3811.672 kb on + strand at 3811.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 3,809,708 + +0.3 3,809,709 - +1.1 3,809,738 + -0.1 3,809,741 + -0.1 3,809,741 + +0.7 3,809,811 + -0.4 3,809,812 - +1.0 3,809,872 + +0.4 3,809,872 + +0.8 3,809,873 - -0.4 3,810,161 + PP_3366 0.31 -0.5 3,810,161 + PP_3366 0.31 +1.1 3,810,167 + PP_3366 0.32 +0.1 3,810,167 + PP_3366 0.32 -1.3 3,810,168 - PP_3366 0.32 -0.9 3,810,168 - PP_3366 0.32 -0.7 3,810,168 - PP_3366 0.32 +0.8 3,810,171 + PP_3366 0.33 -0.8 3,810,196 + PP_3366 0.35 -1.0 3,810,197 - PP_3366 0.35 +0.7 3,810,197 - PP_3366 0.35 -1.0 3,810,197 - PP_3366 0.35 -0.8 3,810,197 - PP_3366 0.35 -1.3 3,810,421 + PP_3366 0.61 -0.4 3,810,421 + PP_3366 0.61 +0.3 3,810,422 - PP_3366 0.61 -1.0 3,810,526 + PP_3366 0.73 -0.2 3,810,526 + PP_3366 0.73 +0.2 3,810,540 - PP_3366 0.74 -0.9 3,810,701 - +0.2 3,810,701 - -0.1 3,810,701 - +0.6 3,810,751 + +0.2 3,810,851 - PP_3367 0.15 -0.1 3,810,852 + PP_3367 0.15 -0.4 3,810,853 - PP_3367 0.16 +0.1 3,810,968 - PP_3367 0.33 -0.3 3,810,970 + PP_3367 0.33 +0.6 3,810,970 + PP_3367 0.33 -0.2 3,810,970 + PP_3367 0.33 -0.3 3,810,985 - PP_3367 0.36 +0.1 3,810,985 - PP_3367 0.36 +1.8 3,811,063 + PP_3367 0.47 -0.8 3,811,213 - PP_3367 0.70 -0.0 3,811,265 + PP_3367 0.78 -0.1 3,811,400 + -0.9 3,811,401 - -0.2 3,811,447 - +0.6 3,811,543 + -0.5 3,811,543 + +0.3 3,811,631 + +0.7 3,811,631 + -0.1 3,811,631 + +1.4 3,811,632 - -0.5 3,811,632 - +0.2 3,811,632 - +1.0 3,811,632 - +0.4 3,811,632 - +0.9 3,811,632 - -0.5 3,811,632 - +0.3 3,811,666 + +0.6 3,811,667 - -0.4 3,811,672 + +0.5 3,811,673 - +0.3
Or see this region's nucleotide sequence