Strain Fitness in Escherichia coli BW25113 around b3945

Experiment: Phage_N4_cage_4_mice _Feces_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyijE and yijF are separated by 26 nucleotidesyijF and gldA are separated by 274 nucleotidesgldA and fsaB are separated by 10 nucleotidesfsaB and ptsA are separated by 11 nucleotides b3943: yijE - orf, hypothetical protein (VIMSS), at 4,134,131 to 4,135,036 yijE b3944: yijF - hypothetical protein (NCBI), at 4,135,063 to 4,135,680 yijF b3945: gldA - glycerol dehydrogenase, (NAD) (VIMSS), at 4,135,955 to 4,137,058 gldA b3946: fsaB - fructose-6-phosphate aldolase 2 (NCBI), at 4,137,069 to 4,137,731 fsaB b3947: ptsA - fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component (NCBI), at 4,137,743 to 4,140,244 ptsA Position (kb) 4135 4136 4137 4138Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4134.960 kb on + strandat 4134.960 kb on + strandat 4134.960 kb on + strandat 4134.989 kb on + strandat 4135.101 kb on + strandat 4135.101 kb on + strandat 4135.171 kb on + strand, within yijFat 4135.171 kb on + strand, within yijFat 4135.196 kb on + strand, within yijFat 4135.220 kb on + strand, within yijFat 4135.235 kb on - strand, within yijFat 4135.258 kb on - strand, within yijFat 4135.310 kb on + strand, within yijFat 4135.333 kb on - strand, within yijFat 4135.333 kb on - strand, within yijFat 4135.455 kb on - strand, within yijFat 4135.479 kb on + strand, within yijFat 4135.479 kb on + strand, within yijFat 4135.502 kb on - strand, within yijFat 4135.516 kb on - strand, within yijFat 4135.528 kb on - strand, within yijFat 4135.528 kb on - strand, within yijFat 4135.548 kb on + strand, within yijFat 4135.556 kb on - strand, within yijFat 4135.556 kb on - strand, within yijFat 4135.556 kb on - strand, within yijFat 4135.572 kb on + strand, within yijFat 4135.572 kb on + strand, within yijFat 4135.577 kb on + strand, within yijFat 4135.580 kb on - strand, within yijFat 4135.596 kb on - strand, within yijFat 4135.696 kb on + strandat 4135.743 kb on - strandat 4135.812 kb on + strandat 4135.812 kb on + strandat 4136.008 kb on - strandat 4136.010 kb on + strandat 4136.010 kb on + strandat 4136.159 kb on + strand, within gldAat 4136.201 kb on + strand, within gldAat 4136.229 kb on - strand, within gldAat 4136.229 kb on - strand, within gldAat 4136.248 kb on + strand, within gldAat 4136.248 kb on + strand, within gldAat 4136.260 kb on - strand, within gldAat 4136.260 kb on - strand, within gldAat 4136.277 kb on - strand, within gldAat 4136.353 kb on + strand, within gldAat 4136.361 kb on - strand, within gldAat 4136.390 kb on - strand, within gldAat 4136.390 kb on - strand, within gldAat 4136.435 kb on + strand, within gldAat 4136.444 kb on - strand, within gldAat 4136.454 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.508 kb on + strand, within gldAat 4136.557 kb on - strand, within gldAat 4136.562 kb on + strand, within gldAat 4136.638 kb on + strand, within gldAat 4136.667 kb on - strand, within gldAat 4136.670 kb on + strand, within gldAat 4136.672 kb on - strand, within gldAat 4136.690 kb on - strand, within gldAat 4136.725 kb on + strand, within gldAat 4136.733 kb on - strand, within gldAat 4136.733 kb on - strand, within gldAat 4136.733 kb on - strand, within gldAat 4136.738 kb on - strand, within gldAat 4136.738 kb on - strand, within gldAat 4136.784 kb on + strand, within gldAat 4136.784 kb on + strand, within gldAat 4136.812 kb on - strand, within gldAat 4136.812 kb on - strand, within gldAat 4136.916 kb on + strandat 4136.970 kb on + strandat 4136.970 kb on + strandat 4137.030 kb on - strandat 4137.061 kb on - strandat 4137.061 kb on - strandat 4137.105 kb on - strandat 4137.105 kb on - strandat 4137.105 kb on - strandat 4137.196 kb on + strand, within fsaBat 4137.214 kb on + strand, within fsaBat 4137.215 kb on - strand, within fsaBat 4137.234 kb on - strand, within fsaBat 4137.288 kb on + strand, within fsaBat 4137.288 kb on + strand, within fsaBat 4137.288 kb on + strand, within fsaBat 4137.291 kb on - strand, within fsaBat 4137.296 kb on - strand, within fsaBat 4137.296 kb on - strand, within fsaBat 4137.344 kb on - strand, within fsaBat 4137.389 kb on - strand, within fsaBat 4137.389 kb on - strand, within fsaBat 4137.393 kb on + strand, within fsaBat 4137.396 kb on + strand, within fsaBat 4137.401 kb on - strand, within fsaBat 4137.401 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.468 kb on - strand, within fsaBat 4137.507 kb on + strand, within fsaBat 4137.525 kb on - strand, within fsaBat 4137.525 kb on - strand, within fsaBat 4137.539 kb on - strand, within fsaBat 4137.539 kb on - strand, within fsaBat 4137.552 kb on + strand, within fsaBat 4137.610 kb on + strand, within fsaBat 4137.647 kb on + strand, within fsaBat 4137.703 kb on + strandat 4137.703 kb on - strandat 4137.705 kb on - strandat 4137.866 kb on - strandat 4137.866 kb on - strandat 4137.900 kb on - strandat 4137.900 kb on - strandat 4137.900 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_4_mice _Feces_40 hr
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4,134,960 + +0.2
4,134,960 + -0.4
4,134,960 + +0.4
4,134,989 + -0.1
4,135,101 + -0.0
4,135,101 + +0.2
4,135,171 + yijF b3944 0.17 -0.6
4,135,171 + yijF b3944 0.17 +0.7
4,135,196 + yijF b3944 0.22 -4.9
4,135,220 + yijF b3944 0.25 -0.4
4,135,235 - yijF b3944 0.28 -0.3
4,135,258 - yijF b3944 0.32 +0.2
4,135,310 + yijF b3944 0.40 +0.9
4,135,333 - yijF b3944 0.44 +0.8
4,135,333 - yijF b3944 0.44 +0.0
4,135,455 - yijF b3944 0.63 -0.9
4,135,479 + yijF b3944 0.67 +0.4
4,135,479 + yijF b3944 0.67 -0.2
4,135,502 - yijF b3944 0.71 -0.0
4,135,516 - yijF b3944 0.73 -0.0
4,135,528 - yijF b3944 0.75 +0.6
4,135,528 - yijF b3944 0.75 -0.2
4,135,548 + yijF b3944 0.78 -0.3
4,135,556 - yijF b3944 0.80 +0.6
4,135,556 - yijF b3944 0.80 +0.3
4,135,556 - yijF b3944 0.80 -0.4
4,135,572 + yijF b3944 0.82 -1.4
4,135,572 + yijF b3944 0.82 +0.1
4,135,577 + yijF b3944 0.83 -0.3
4,135,580 - yijF b3944 0.84 +0.1
4,135,596 - yijF b3944 0.86 -1.2
4,135,696 + +0.0
4,135,743 - +0.2
4,135,812 + +0.7
4,135,812 + -0.3
4,136,008 - -0.4
4,136,010 + +0.7
4,136,010 + -0.3
4,136,159 + gldA b3945 0.18 -0.0
4,136,201 + gldA b3945 0.22 -0.5
4,136,229 - gldA b3945 0.25 -0.3
4,136,229 - gldA b3945 0.25 +0.2
4,136,248 + gldA b3945 0.27 -0.7
4,136,248 + gldA b3945 0.27 -0.2
4,136,260 - gldA b3945 0.28 -0.0
4,136,260 - gldA b3945 0.28 -0.0
4,136,277 - gldA b3945 0.29 +0.9
4,136,353 + gldA b3945 0.36 -1.2
4,136,361 - gldA b3945 0.37 -0.0
4,136,390 - gldA b3945 0.39 -0.2
4,136,390 - gldA b3945 0.39 -0.5
4,136,435 + gldA b3945 0.43 -0.1
4,136,444 - gldA b3945 0.44 +0.2
4,136,454 - gldA b3945 0.45 +0.3
4,136,474 - gldA b3945 0.47 +0.5
4,136,474 - gldA b3945 0.47 +0.0
4,136,474 - gldA b3945 0.47 -0.6
4,136,474 - gldA b3945 0.47 +0.5
4,136,508 + gldA b3945 0.50 -1.1
4,136,557 - gldA b3945 0.55 -0.8
4,136,562 + gldA b3945 0.55 +0.6
4,136,638 + gldA b3945 0.62 +0.1
4,136,667 - gldA b3945 0.64 +0.5
4,136,670 + gldA b3945 0.65 -0.4
4,136,672 - gldA b3945 0.65 -0.3
4,136,690 - gldA b3945 0.67 -0.5
4,136,725 + gldA b3945 0.70 -0.0
4,136,733 - gldA b3945 0.70 -0.5
4,136,733 - gldA b3945 0.70 +0.6
4,136,733 - gldA b3945 0.70 +0.1
4,136,738 - gldA b3945 0.71 +0.7
4,136,738 - gldA b3945 0.71 -0.1
4,136,784 + gldA b3945 0.75 +0.7
4,136,784 + gldA b3945 0.75 +0.9
4,136,812 - gldA b3945 0.78 +0.8
4,136,812 - gldA b3945 0.78 -0.3
4,136,916 + -1.4
4,136,970 + -0.3
4,136,970 + +0.2
4,137,030 - +0.6
4,137,061 - +0.2
4,137,061 - +0.6
4,137,105 - +0.4
4,137,105 - +0.1
4,137,105 - -0.8
4,137,196 + fsaB b3946 0.19 +0.4
4,137,214 + fsaB b3946 0.22 +0.4
4,137,215 - fsaB b3946 0.22 +0.1
4,137,234 - fsaB b3946 0.25 -0.1
4,137,288 + fsaB b3946 0.33 +0.5
4,137,288 + fsaB b3946 0.33 -0.2
4,137,288 + fsaB b3946 0.33 +1.5
4,137,291 - fsaB b3946 0.33 -0.7
4,137,296 - fsaB b3946 0.34 +0.4
4,137,296 - fsaB b3946 0.34 -2.5
4,137,344 - fsaB b3946 0.41 -1.5
4,137,389 - fsaB b3946 0.48 +0.5
4,137,389 - fsaB b3946 0.48 -0.0
4,137,393 + fsaB b3946 0.49 +0.1
4,137,396 + fsaB b3946 0.49 +0.4
4,137,401 - fsaB b3946 0.50 -0.5
4,137,401 - fsaB b3946 0.50 +0.1
4,137,404 - fsaB b3946 0.51 -0.2
4,137,404 - fsaB b3946 0.51 +0.4
4,137,404 - fsaB b3946 0.51 -0.6
4,137,404 - fsaB b3946 0.51 -0.6
4,137,468 - fsaB b3946 0.60 +0.6
4,137,507 + fsaB b3946 0.66 +0.7
4,137,525 - fsaB b3946 0.69 +1.1
4,137,525 - fsaB b3946 0.69 -0.2
4,137,539 - fsaB b3946 0.71 +0.4
4,137,539 - fsaB b3946 0.71 -0.7
4,137,552 + fsaB b3946 0.73 -0.4
4,137,610 + fsaB b3946 0.82 +0.6
4,137,647 + fsaB b3946 0.87 -1.2
4,137,703 + +0.6
4,137,703 - +0.1
4,137,705 - -0.2
4,137,866 - -0.2
4,137,866 - +0.1
4,137,900 - +0.6
4,137,900 - +0.4
4,137,900 - -0.7

Or see this region's nucleotide sequence