Strain Fitness in Escherichia coli BW25113 around b2417

Experiment: Phage_N4_cage_4_mice _Feces_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntptsI and crr are separated by 40 nucleotidescrr and pdxK are separated by 42 nucleotidespdxK and yfeK are separated by 104 nucleotides b2416: ptsI - PEP-protein phosphotransferase of PTS system (enzyme I) (NCBI), at 2,532,088 to 2,533,815 ptsI b2417: crr - glucose-specific PTS system enzyme IIA component (NCBI), at 2,533,856 to 2,534,365 crr b2418: pdxK - pyridoxine kinase (NCBI), at 2,534,408 to 2,535,259 pdxK b2419: yfeK - hypothetical protein (NCBI), at 2,535,364 to 2,535,738 yfeK Position (kb) 2533 2534 2535Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2534.053 kb on - strand, within crrat 2534.468 kb on - strandat 2534.488 kb on + strandat 2534.506 kb on - strand, within pdxKat 2534.519 kb on - strand, within pdxKat 2534.519 kb on - strand, within pdxKat 2534.591 kb on - strand, within pdxKat 2534.591 kb on - strand, within pdxKat 2534.601 kb on + strand, within pdxKat 2534.601 kb on + strand, within pdxKat 2534.618 kb on + strand, within pdxKat 2534.618 kb on + strand, within pdxKat 2534.708 kb on - strand, within pdxKat 2534.980 kb on + strand, within pdxKat 2535.016 kb on + strand, within pdxKat 2535.103 kb on - strand, within pdxKat 2535.120 kb on + strand, within pdxKat 2535.137 kb on + strand, within pdxKat 2535.137 kb on + strand, within pdxKat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strandat 2535.230 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_4_mice _Feces_40 hr
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2,534,053 - crr b2417 0.39 +1.2
2,534,468 - +1.4
2,534,488 + -0.3
2,534,506 - pdxK b2418 0.12 -0.0
2,534,519 - pdxK b2418 0.13 +0.2
2,534,519 - pdxK b2418 0.13 -0.0
2,534,591 - pdxK b2418 0.21 -0.3
2,534,591 - pdxK b2418 0.21 -0.4
2,534,601 + pdxK b2418 0.23 -1.3
2,534,601 + pdxK b2418 0.23 -0.1
2,534,618 + pdxK b2418 0.25 -3.5
2,534,618 + pdxK b2418 0.25 +0.2
2,534,708 - pdxK b2418 0.35 +0.3
2,534,980 + pdxK b2418 0.67 -0.0
2,535,016 + pdxK b2418 0.71 -1.2
2,535,103 - pdxK b2418 0.82 +0.0
2,535,120 + pdxK b2418 0.84 +0.6
2,535,137 + pdxK b2418 0.86 +0.3
2,535,137 + pdxK b2418 0.86 -0.7
2,535,230 - +0.1
2,535,230 - -0.6
2,535,230 - +3.9
2,535,230 - -0.3
2,535,230 - -0.6
2,535,230 - -0.4
2,535,230 - +0.5
2,535,230 - +0.3

Or see this region's nucleotide sequence