Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2841 and PP_2842 are separated by 277 nucleotides PP_2842 and PP_2843 are separated by 9 nucleotides PP_2843 and PP_2844 are separated by 19 nucleotides PP_2844 and PP_2845 overlap by 4 nucleotides
PP_2841: PP_2841 - Site-specific recombinase, phage integrase family, at 3,245,746 to 3,246,624
_2841
PP_2842: PP_2842 - urease accessory protein, at 3,246,902 to 3,247,735
_2842
PP_2843: PP_2843 - urease subunit gamma, at 3,247,745 to 3,248,047
_2843
PP_2844: PP_2844 - Urease subunit beta, at 3,248,067 to 3,248,384
_2844
PP_2845: PP_2845 - urease subunit alpha, at 3,248,381 to 3,250,084
_2845
Position (kb)
3246
3247
3248 Strain fitness (log2 ratio)
-2
-1
0
1 at 3245.932 kb on + strand, within PP_2841 at 3245.933 kb on - strand, within PP_2841 at 3245.933 kb on - strand, within PP_2841 at 3245.933 kb on - strand, within PP_2841 at 3246.006 kb on + strand, within PP_2841 at 3246.122 kb on + strand, within PP_2841 at 3246.122 kb on + strand, within PP_2841 at 3246.122 kb on + strand, within PP_2841 at 3246.262 kb on + strand, within PP_2841 at 3246.415 kb on + strand, within PP_2841 at 3246.415 kb on + strand, within PP_2841 at 3246.415 kb on + strand, within PP_2841 at 3246.418 kb on + strand, within PP_2841 at 3246.418 kb on + strand, within PP_2841 at 3246.418 kb on + strand, within PP_2841 at 3246.568 kb on + strand at 3246.568 kb on + strand at 3246.569 kb on - strand at 3246.569 kb on - strand at 3246.632 kb on + strand at 3246.633 kb on - strand at 3246.666 kb on - strand at 3246.756 kb on + strand at 3246.757 kb on - strand at 3246.794 kb on + strand at 3246.795 kb on - strand at 3246.797 kb on - strand at 3247.046 kb on + strand, within PP_2842 at 3247.073 kb on + strand, within PP_2842 at 3247.073 kb on + strand, within PP_2842 at 3247.073 kb on + strand, within PP_2842 at 3247.073 kb on + strand, within PP_2842 at 3247.074 kb on - strand, within PP_2842 at 3247.074 kb on - strand, within PP_2842 at 3247.074 kb on - strand, within PP_2842 at 3247.185 kb on - strand, within PP_2842 at 3247.500 kb on + strand, within PP_2842 at 3247.561 kb on - strand, within PP_2842 at 3247.729 kb on + strand at 3247.838 kb on + strand, within PP_2843 at 3248.193 kb on + strand, within PP_2844 at 3248.194 kb on - strand, within PP_2844 at 3248.194 kb on - strand, within PP_2844 at 3248.316 kb on + strand, within PP_2844 at 3248.337 kb on + strand, within PP_2844 at 3248.338 kb on - strand at 3248.338 kb on - strand, within PP_2844 at 3248.389 kb on + strand at 3248.495 kb on + strand at 3248.496 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 3,245,932 + PP_2841 0.21 +0.8 3,245,933 - PP_2841 0.21 -0.8 3,245,933 - PP_2841 0.21 -1.2 3,245,933 - PP_2841 0.21 +1.2 3,246,006 + PP_2841 0.30 +0.2 3,246,122 + PP_2841 0.43 +0.1 3,246,122 + PP_2841 0.43 +0.1 3,246,122 + PP_2841 0.43 -1.2 3,246,262 + PP_2841 0.59 +0.7 3,246,415 + PP_2841 0.76 +0.0 3,246,415 + PP_2841 0.76 +0.3 3,246,415 + PP_2841 0.76 +0.6 3,246,418 + PP_2841 0.76 -1.1 3,246,418 + PP_2841 0.76 +0.0 3,246,418 + PP_2841 0.76 +0.4 3,246,568 + +1.1 3,246,568 + +0.1 3,246,569 - +0.1 3,246,569 - -0.6 3,246,632 + +0.0 3,246,633 - +0.1 3,246,666 - -0.3 3,246,756 + -1.4 3,246,757 - +0.8 3,246,794 + -1.6 3,246,795 - +0.8 3,246,797 - +0.8 3,247,046 + PP_2842 0.17 +0.1 3,247,073 + PP_2842 0.21 +0.1 3,247,073 + PP_2842 0.21 -0.5 3,247,073 + PP_2842 0.21 +1.6 3,247,073 + PP_2842 0.21 +0.6 3,247,074 - PP_2842 0.21 +0.3 3,247,074 - PP_2842 0.21 +0.5 3,247,074 - PP_2842 0.21 +1.2 3,247,185 - PP_2842 0.34 +0.1 3,247,500 + PP_2842 0.72 +0.6 3,247,561 - PP_2842 0.79 -0.6 3,247,729 + +0.3 3,247,838 + PP_2843 0.31 -0.1 3,248,193 + PP_2844 0.40 +0.1 3,248,194 - PP_2844 0.40 +0.3 3,248,194 - PP_2844 0.40 -0.6 3,248,316 + PP_2844 0.78 +0.8 3,248,337 + PP_2844 0.85 -1.9 3,248,338 - -1.1 3,248,338 - PP_2844 0.85 -0.1 3,248,389 + -0.5 3,248,495 + -0.6 3,248,496 - -0.0
Or see this region's nucleotide sequence