Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2052 and PP_5500 are separated by 79 nucleotides PP_5500 and PP_2053 are separated by 113 nucleotides PP_2053 and PP_2054 are separated by 18 nucleotides PP_2054 and PP_2055 are separated by 146 nucleotides
PP_2052: PP_2052 - putative bifunctional enzyme: sugar-phosphatase/mannitol-1-phosphate 5-dehydrogenase, at 2,334,333 to 2,336,456
_2052
PP_5500: PP_5500 - protein of unknown function, at 2,336,536 to 2,336,739
_5500
PP_2053: PP_2053 - conserved protein of unknown function, at 2,336,853 to 2,337,149
_2053
PP_2054: PP_2054 - putative DNA-binding transcriptional regulator, at 2,337,168 to 2,338,061
_2054
PP_2055: PP_2055 - putative isomerase, at 2,338,208 to 2,339,287
_2055
Position (kb)
2337
2338
2339 Strain fitness (log2 ratio)
-2
-1
0
1 at 2336.178 kb on + strand, within PP_2052 at 2336.197 kb on + strand, within PP_2052 at 2336.197 kb on + strand, within PP_2052 at 2336.198 kb on - strand, within PP_2052 at 2336.198 kb on - strand, within PP_2052 at 2336.239 kb on + strand at 2336.589 kb on - strand, within PP_5500 at 2336.589 kb on - strand, within PP_5500 at 2336.711 kb on + strand, within PP_5500 at 2336.712 kb on - strand, within PP_5500 at 2336.721 kb on + strand at 2336.721 kb on + strand at 2336.722 kb on - strand at 2336.722 kb on - strand at 2336.833 kb on - strand at 2336.880 kb on + strand at 2336.880 kb on + strand at 2336.950 kb on + strand, within PP_2053 at 2336.951 kb on - strand, within PP_2053 at 2336.951 kb on - strand, within PP_2053 at 2336.951 kb on - strand, within PP_2053 at 2336.951 kb on - strand, within PP_2053 at 2336.951 kb on - strand, within PP_2053 at 2337.073 kb on - strand, within PP_2053 at 2337.107 kb on + strand, within PP_2053 at 2337.107 kb on + strand, within PP_2053 at 2337.107 kb on + strand, within PP_2053 at 2337.138 kb on - strand at 2337.245 kb on - strand at 2337.255 kb on + strand at 2337.255 kb on + strand at 2337.400 kb on + strand, within PP_2054 at 2337.559 kb on - strand, within PP_2054 at 2337.719 kb on - strand, within PP_2054 at 2337.746 kb on - strand, within PP_2054 at 2338.055 kb on - strand at 2338.140 kb on + strand at 2338.227 kb on + strand at 2338.227 kb on + strand at 2338.227 kb on + strand at 2338.536 kb on + strand, within PP_2055 at 2338.537 kb on - strand, within PP_2055 at 2338.982 kb on + strand, within PP_2055 at 2339.006 kb on + strand, within PP_2055 at 2339.006 kb on + strand, within PP_2055 at 2339.007 kb on - strand, within PP_2055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,336,178 + PP_2052 0.87 +0.9 2,336,197 + PP_2052 0.88 +0.2 2,336,197 + PP_2052 0.88 -0.7 2,336,198 - PP_2052 0.88 +1.0 2,336,198 - PP_2052 0.88 -0.8 2,336,239 + -0.8 2,336,589 - PP_5500 0.26 -0.3 2,336,589 - PP_5500 0.26 -1.3 2,336,711 + PP_5500 0.86 +0.9 2,336,712 - PP_5500 0.86 -0.8 2,336,721 + -0.6 2,336,721 + +0.9 2,336,722 - +0.5 2,336,722 - +0.3 2,336,833 - +1.3 2,336,880 + -1.8 2,336,880 + -0.2 2,336,950 + PP_2053 0.33 -0.1 2,336,951 - PP_2053 0.33 +0.5 2,336,951 - PP_2053 0.33 +0.3 2,336,951 - PP_2053 0.33 -0.8 2,336,951 - PP_2053 0.33 -0.1 2,336,951 - PP_2053 0.33 -0.3 2,337,073 - PP_2053 0.74 -0.0 2,337,107 + PP_2053 0.86 -2.0 2,337,107 + PP_2053 0.86 -0.9 2,337,107 + PP_2053 0.86 -0.1 2,337,138 - +0.1 2,337,245 - +0.5 2,337,255 + +0.1 2,337,255 + +1.4 2,337,400 + PP_2054 0.26 +0.6 2,337,559 - PP_2054 0.44 +0.6 2,337,719 - PP_2054 0.62 +0.2 2,337,746 - PP_2054 0.65 +0.2 2,338,055 - -0.9 2,338,140 + +1.6 2,338,227 + -0.4 2,338,227 + -0.4 2,338,227 + +1.2 2,338,536 + PP_2055 0.30 -1.3 2,338,537 - PP_2055 0.30 +0.2 2,338,982 + PP_2055 0.72 -0.3 2,339,006 + PP_2055 0.74 -0.5 2,339,006 + PP_2055 0.74 -0.1 2,339,007 - PP_2055 0.74 +0.2
Or see this region's nucleotide sequence