Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1900 and PP_1901 are separated by 44 nucleotides PP_1901 and PP_1902 overlap by 4 nucleotides PP_1902 and PP_1903 overlap by 1 nucleotides PP_1903 and PP_1904 overlap by 40 nucleotides
PP_1900: PP_1900 - Tetraacyldisaccharide 4'-kinase, at 2,143,652 to 2,144,656
_1900
PP_1901: PP_1901 - conserved protein of unknown function, UPF0434 family, at 2,144,701 to 2,144,886
_1901
PP_1902: PP_1902 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,144,883 to 2,145,647
_1902
PP_1903: PP_1903 - putative phosphotyrosine-protein phosphatase, at 2,145,647 to 2,146,111
_1903
PP_1904: PP_1904 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 2,146,072 to 2,147,127
_1904
Position (kb) 2145
2146
2147 Strain fitness (log2 ratio) -1
0
1 at 2144.686 kb on + strand at 2145.887 kb on + strand, within PP_1903 at 2146.019 kb on + strand, within PP_1903 at 2146.022 kb on + strand, within PP_1903 at 2146.022 kb on + strand, within PP_1903 at 2146.022 kb on + strand, within PP_1903 at 2146.107 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 2,144,686 + +1.6 2,145,887 + PP_1903 0.52 +0.2 2,146,019 + PP_1903 0.80 +1.1 2,146,022 + PP_1903 0.81 +0.2 2,146,022 + PP_1903 0.81 +1.5 2,146,022 + PP_1903 0.81 +0.0 2,146,107 + -0.1
Or see this region's nucleotide sequence