Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1068 and PP_1069 overlap by 40 nucleotides PP_1069 and PP_1070 are separated by 0 nucleotides PP_1070 and PP_1071 are separated by 145 nucleotides
PP_1068: PP_1068 - glutamate / aspartate ABC transporter - ATP binding subunit, at 1,223,275 to 1,224,045
_1068
PP_1069: PP_1069 - glutamate / aspartate ABC transporter - permease subunit, at 1,224,006 to 1,224,677
_1069
PP_1070: PP_1070 - glutamate / aspartate ABC transporter - permease subunit, at 1,224,678 to 1,225,424
_1070
PP_1071: PP_1071 - glutamate / aspartate ABC transporter - periplasmic binding protein, at 1,225,570 to 1,226,490
_1071
Position (kb)
1224
1225
1226 Strain fitness (log2 ratio)
-1
0
1
2 at 1224.070 kb on + strand at 1224.166 kb on + strand, within PP_1069 at 1224.166 kb on + strand, within PP_1069 at 1224.167 kb on - strand, within PP_1069 at 1224.167 kb on - strand, within PP_1069 at 1224.168 kb on + strand, within PP_1069 at 1224.271 kb on + strand, within PP_1069 at 1224.272 kb on - strand, within PP_1069 at 1224.410 kb on + strand, within PP_1069 at 1224.454 kb on + strand, within PP_1069 at 1224.454 kb on + strand, within PP_1069 at 1224.455 kb on - strand at 1224.497 kb on - strand, within PP_1069 at 1224.497 kb on - strand, within PP_1069 at 1224.680 kb on - strand at 1224.769 kb on + strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.770 kb on - strand, within PP_1070 at 1224.958 kb on + strand, within PP_1070 at 1224.958 kb on + strand, within PP_1070 at 1224.959 kb on - strand, within PP_1070 at 1224.959 kb on - strand, within PP_1070 at 1224.959 kb on - strand, within PP_1070 at 1225.172 kb on - strand, within PP_1070 at 1225.223 kb on - strand, within PP_1070 at 1225.351 kb on + strand at 1225.352 kb on - strand at 1225.363 kb on + strand at 1225.418 kb on - strand at 1225.418 kb on - strand at 1225.502 kb on + strand at 1225.503 kb on - strand at 1225.503 kb on - strand at 1225.509 kb on + strand at 1225.557 kb on + strand at 1225.557 kb on + strand at 1225.561 kb on + strand at 1225.561 kb on + strand at 1225.562 kb on - strand at 1225.695 kb on - strand, within PP_1071 at 1225.724 kb on + strand, within PP_1071 at 1226.058 kb on - strand, within PP_1071 at 1226.058 kb on - strand, within PP_1071 at 1226.206 kb on - strand, within PP_1071 at 1226.232 kb on - strand, within PP_1071 at 1226.232 kb on - strand, within PP_1071 at 1226.232 kb on - strand, within PP_1071 at 1226.232 kb on - strand, within PP_1071 at 1226.333 kb on + strand, within PP_1071
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 1,224,070 + +0.1 1,224,166 + PP_1069 0.24 +0.4 1,224,166 + PP_1069 0.24 -0.9 1,224,167 - PP_1069 0.24 -0.3 1,224,167 - PP_1069 0.24 -0.1 1,224,168 + PP_1069 0.24 -0.9 1,224,271 + PP_1069 0.39 -0.0 1,224,272 - PP_1069 0.40 -0.5 1,224,410 + PP_1069 0.60 +1.3 1,224,454 + PP_1069 0.67 +0.7 1,224,454 + PP_1069 0.67 +0.4 1,224,455 - +1.1 1,224,497 - PP_1069 0.73 -0.2 1,224,497 - PP_1069 0.73 +0.7 1,224,680 - +0.1 1,224,769 + PP_1070 0.12 +1.5 1,224,770 - PP_1070 0.12 +0.4 1,224,770 - PP_1070 0.12 +0.3 1,224,770 - PP_1070 0.12 +0.2 1,224,770 - PP_1070 0.12 +0.5 1,224,958 + PP_1070 0.37 +0.1 1,224,958 + PP_1070 0.37 +1.9 1,224,959 - PP_1070 0.38 -0.3 1,224,959 - PP_1070 0.38 +0.5 1,224,959 - PP_1070 0.38 -0.4 1,225,172 - PP_1070 0.66 +0.9 1,225,223 - PP_1070 0.73 +0.3 1,225,351 + -0.2 1,225,352 - +0.1 1,225,363 + -0.5 1,225,418 - -0.5 1,225,418 - -0.2 1,225,502 + -1.3 1,225,503 - +0.7 1,225,503 - -0.4 1,225,509 + -0.0 1,225,557 + -0.4 1,225,557 + -0.4 1,225,561 + +0.1 1,225,561 + -0.3 1,225,562 - -0.1 1,225,695 - PP_1071 0.14 +0.3 1,225,724 + PP_1071 0.17 -0.3 1,226,058 - PP_1071 0.53 +0.8 1,226,058 - PP_1071 0.53 -0.5 1,226,206 - PP_1071 0.69 +1.0 1,226,232 - PP_1071 0.72 +0.1 1,226,232 - PP_1071 0.72 +0.4 1,226,232 - PP_1071 0.72 -0.4 1,226,232 - PP_1071 0.72 +0.3 1,226,333 + PP_1071 0.83 -0.1
Or see this region's nucleotide sequence