Experiment: Phage_N4_cage_3_mice _Feces_22 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplS and trmD are separated by 41 nucleotides trmD and yfjA are separated by 30 nucleotides yfjA and rpsP are separated by 18 nucleotides rpsP and ffh are separated by 248 nucleotides
b2606: rplS - 50S ribosomal protein L19 (NCBI), at 2,742,205 to 2,742,552
rplS
b2607: trmD - tRNA (guanine-N(1)-)-methyltransferase (NCBI), at 2,742,594 to 2,743,361
trmD
b2608: yfjA - orf, hypothetical protein (VIMSS), at 2,743,392 to 2,743,940
yfjA
b2609: rpsP - 30S ribosomal protein S16 (NCBI), at 2,743,959 to 2,744,207
rpsP
b2610: ffh - Signal Recognition Particle (SRP) component with 4.5S RNA (ffs) (NCBI), at 2,744,456 to 2,745,817
ffh
Position (kb)
2743
2744 Strain fitness (log2 ratio)
-1
0
1 at 2742.626 kb on - strand at 2743.986 kb on - strand, within rpsP at 2743.986 kb on - strand, within rpsP at 2744.337 kb on - strand at 2744.337 kb on - strand at 2744.358 kb on - strand at 2744.358 kb on - strand at 2744.400 kb on + strand at 2744.417 kb on + strand at 2744.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mice _Feces_22 hr remove 2,742,626 - -0.3 2,743,986 - rpsP b2609 0.11 -1.0 2,743,986 - rpsP b2609 0.11 +0.3 2,744,337 - -0.5 2,744,337 - +0.1 2,744,358 - +1.0 2,744,358 - +0.3 2,744,400 + -0.5 2,744,417 + +0.2 2,744,417 - +0.4
Or see this region's nucleotide sequence