Strain Fitness in Escherichia coli BW25113 around b1011

Experiment: PBS_cage_2_mice _Feces_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrarA and ycdK are separated by 7 nucleotidesycdK and b1011 are separated by 11 nucleotidesb1011 and b1012 overlap by 1 nucleotides b1009: rarA - predicted hydrolase (NCBI), at 1,070,188 to 1,070,988 rarA b1010: ycdK - hypothetical protein (NCBI), at 1,070,996 to 1,071,382 ycdK b1011: b1011 - putative synthetase (VIMSS), at 1,071,394 to 1,072,086 b1011 b1012: b1012 - orf, hypothetical protein (VIMSS), at 1,072,086 to 1,073,234 b1012 Position (kb) 1071 1072 1073Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 1070.569 kb on - strand, within rarAat 1070.580 kb on - strand, within rarAat 1070.580 kb on - strand, within rarAat 1070.633 kb on - strand, within rarAat 1070.633 kb on - strand, within rarAat 1070.678 kb on - strand, within rarAat 1070.684 kb on + strand, within rarAat 1070.689 kb on + strand, within rarAat 1070.840 kb on + strand, within rarAat 1070.863 kb on - strand, within rarAat 1070.875 kb on - strand, within rarAat 1070.875 kb on - strand, within rarAat 1070.956 kb on + strandat 1070.956 kb on + strandat 1070.988 kb on + strandat 1071.230 kb on - strand, within ycdKat 1071.301 kb on - strand, within ycdKat 1071.301 kb on - strand, within ycdKat 1071.301 kb on - strand, within ycdKat 1071.319 kb on - strand, within ycdKat 1071.343 kb on - strand, within ycdKat 1071.343 kb on - strand, within ycdKat 1071.382 kb on + strandat 1071.696 kb on + strand, within b1011at 1071.712 kb on + strand, within b1011at 1071.737 kb on - strand, within b1011at 1072.068 kb on + strandat 1072.068 kb on + strandat 1072.104 kb on - strandat 1072.104 kb on - strandat 1072.188 kb on + strandat 1072.404 kb on - strand, within b1012at 1072.404 kb on - strand, within b1012at 1072.477 kb on - strand, within b1012at 1072.477 kb on - strand, within b1012at 1072.730 kb on - strand, within b1012at 1072.755 kb on - strand, within b1012

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Per-strain Table

Position Strand Gene LocusTag Fraction PBS_cage_2_mice _Feces_40 hr
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1,070,569 - rarA b1009 0.48 -0.5
1,070,580 - rarA b1009 0.49 +0.2
1,070,580 - rarA b1009 0.49 +1.2
1,070,633 - rarA b1009 0.56 +1.6
1,070,633 - rarA b1009 0.56 -0.6
1,070,678 - rarA b1009 0.61 +0.0
1,070,684 + rarA b1009 0.62 -0.5
1,070,689 + rarA b1009 0.63 -0.6
1,070,840 + rarA b1009 0.81 -0.2
1,070,863 - rarA b1009 0.84 -2.8
1,070,875 - rarA b1009 0.86 -1.3
1,070,875 - rarA b1009 0.86 -1.7
1,070,956 + +0.3
1,070,956 + +1.3
1,070,988 + +1.2
1,071,230 - ycdK b1010 0.60 +1.1
1,071,301 - ycdK b1010 0.79 +0.6
1,071,301 - ycdK b1010 0.79 -0.2
1,071,301 - ycdK b1010 0.79 +1.0
1,071,319 - ycdK b1010 0.83 +1.1
1,071,343 - ycdK b1010 0.90 -0.1
1,071,343 - ycdK b1010 0.90 +3.7
1,071,382 + +0.8
1,071,696 + b1011 b1011 0.44 +1.1
1,071,712 + b1011 b1011 0.46 +1.0
1,071,737 - b1011 b1011 0.49 +0.3
1,072,068 + +0.5
1,072,068 + -2.3
1,072,104 - -0.2
1,072,104 - -2.2
1,072,188 + +0.3
1,072,404 - b1012 b1012 0.28 -5.0
1,072,404 - b1012 b1012 0.28 +2.1
1,072,477 - b1012 b1012 0.34 +0.6
1,072,477 - b1012 b1012 0.34 +0.2
1,072,730 - b1012 b1012 0.56 -0.5
1,072,755 - b1012 b1012 0.58 +0.4

Or see this region's nucleotide sequence