Experiment: PBS_cage_1_mice _Feces_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potH and potI overlap by 4 nucleotides potI and ybjO are separated by 59 nucleotides ybjO and rumB are separated by 40 nucleotides
b0856: potH - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 895,357 to 896,310
potH
b0857: potI - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 896,307 to 897,152
potI
b0858: ybjO - predicted inner membrane protein (NCBI), at 897,212 to 897,700
ybjO
b0859: rumB - 23S rRNA m(5)U747-methyltransferase (NCBI), at 897,741 to 898,868
rumB
Position (kb) 897
898 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 896.425 kb on + strand, within potI at 896.425 kb on + strand, within potI at 896.429 kb on + strand, within potI at 896.429 kb on + strand, within potI at 896.497 kb on - strand, within potI at 896.497 kb on - strand, within potI at 896.607 kb on - strand, within potI at 896.607 kb on - strand, within potI at 897.041 kb on + strand, within potI at 897.053 kb on - strand, within potI at 897.084 kb on - strand at 897.084 kb on - strand at 897.371 kb on - strand, within ybjO at 897.402 kb on - strand, within ybjO at 897.426 kb on + strand, within ybjO at 897.702 kb on - strand at 897.724 kb on + strand at 897.744 kb on - strand at 897.780 kb on - strand at 897.815 kb on - strand at 897.815 kb on - strand at 897.894 kb on + strand, within rumB at 897.894 kb on + strand, within rumB at 897.929 kb on + strand, within rumB at 897.929 kb on + strand, within rumB at 897.953 kb on + strand, within rumB at 897.956 kb on - strand, within rumB at 897.960 kb on + strand, within rumB at 898.006 kb on - strand, within rumB at 898.006 kb on - strand, within rumB at 898.164 kb on - strand, within rumB at 898.193 kb on - strand, within rumB at 898.201 kb on - strand, within rumB at 898.201 kb on - strand, within rumB at 898.293 kb on + strand, within rumB at 898.331 kb on + strand, within rumB at 898.331 kb on + strand, within rumB at 898.455 kb on + strand, within rumB at 898.455 kb on + strand, within rumB at 898.468 kb on + strand, within rumB at 898.468 kb on - strand, within rumB at 898.482 kb on + strand, within rumB at 898.490 kb on - strand, within rumB at 898.490 kb on - strand, within rumB at 898.497 kb on + strand, within rumB at 898.498 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.700 kb on + strand, within rumB at 898.700 kb on + strand, within rumB
Per-strain Table
Position Strand Gene LocusTag Fraction PBS_cage_1_mice _Feces_40 hr remove 896,425 + potI b0857 0.14 -3.6 896,425 + potI b0857 0.14 +0.8 896,429 + potI b0857 0.14 -1.0 896,429 + potI b0857 0.14 +0.6 896,497 - potI b0857 0.22 -1.9 896,497 - potI b0857 0.22 -1.3 896,607 - potI b0857 0.35 -1.5 896,607 - potI b0857 0.35 -3.6 897,041 + potI b0857 0.87 -0.2 897,053 - potI b0857 0.88 +0.1 897,084 - +2.9 897,084 - +0.6 897,371 - ybjO b0858 0.33 -4.7 897,402 - ybjO b0858 0.39 -0.4 897,426 + ybjO b0858 0.44 -1.6 897,702 - +1.0 897,724 + -1.5 897,744 - -0.2 897,780 - -2.5 897,815 - -0.4 897,815 - -2.2 897,894 + rumB b0859 0.14 -0.3 897,894 + rumB b0859 0.14 -1.0 897,929 + rumB b0859 0.17 -0.4 897,929 + rumB b0859 0.17 +1.0 897,953 + rumB b0859 0.19 +1.9 897,956 - rumB b0859 0.19 -3.9 897,960 + rumB b0859 0.19 +0.0 898,006 - rumB b0859 0.23 -1.9 898,006 - rumB b0859 0.23 -1.7 898,164 - rumB b0859 0.38 +0.2 898,193 - rumB b0859 0.40 -0.1 898,201 - rumB b0859 0.41 -2.7 898,201 - rumB b0859 0.41 +1.8 898,293 + rumB b0859 0.49 +1.3 898,331 + rumB b0859 0.52 +0.8 898,331 + rumB b0859 0.52 -0.6 898,455 + rumB b0859 0.63 -1.6 898,455 + rumB b0859 0.63 -0.6 898,468 + rumB b0859 0.64 -0.3 898,468 - rumB b0859 0.64 -1.0 898,482 + rumB b0859 0.66 -1.3 898,490 - rumB b0859 0.66 -0.5 898,490 - rumB b0859 0.66 -0.9 898,497 + rumB b0859 0.67 +1.7 898,498 + rumB b0859 0.67 -0.9 898,549 + rumB b0859 0.72 -1.6 898,549 + rumB b0859 0.72 -0.8 898,549 + rumB b0859 0.72 -1.5 898,700 + rumB b0859 0.85 -1.0 898,700 + rumB b0859 0.85 +0.1
Or see this region's nucleotide sequence