Experiment: PBS_cage_1_mice _Feces_22 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjL and puuR are separated by 26 nucleotides puuR and puuC are separated by 274 nucleotides puuC and puuB are separated by 1 nucleotides
b1298: ycjL - probable amidotransferase subunit (VIMSS), at 1,359,144 to 1,359,908
ycjL
b1299: puuR - DNA-binding transcriptional repressor (NCBI), at 1,359,935 to 1,360,492
puuR
b1300: puuC - gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI), at 1,360,767 to 1,362,254
puuC
b1301: puuB - gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI), at 1,362,256 to 1,363,536
puuB
Position (kb)
1360
1361
1362
1363 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1360.081 kb on - strand, within puuR at 1360.084 kb on - strand, within puuR at 1360.317 kb on - strand, within puuR at 1360.332 kb on - strand, within puuR at 1360.334 kb on + strand, within puuR at 1360.413 kb on - strand, within puuR at 1360.478 kb on - strand at 1360.478 kb on - strand at 1360.521 kb on - strand at 1360.526 kb on - strand at 1360.526 kb on - strand at 1360.698 kb on - strand at 1360.698 kb on - strand at 1360.761 kb on - strand at 1360.903 kb on - strand at 1360.903 kb on - strand at 1360.933 kb on - strand, within puuC at 1361.146 kb on + strand, within puuC at 1361.146 kb on + strand, within puuC at 1361.262 kb on - strand, within puuC at 1361.563 kb on - strand, within puuC at 1361.674 kb on - strand, within puuC at 1361.727 kb on - strand, within puuC at 1362.160 kb on + strand at 1362.229 kb on - strand at 1362.317 kb on + strand at 1362.520 kb on + strand, within puuB at 1362.664 kb on + strand, within puuB at 1362.716 kb on - strand, within puuB at 1362.757 kb on + strand, within puuB
Per-strain Table
Position Strand Gene LocusTag Fraction PBS_cage_1_mice _Feces_22 hr remove 1,360,081 - puuR b1299 0.26 -0.1 1,360,084 - puuR b1299 0.27 -1.0 1,360,317 - puuR b1299 0.68 -1.5 1,360,332 - puuR b1299 0.71 -3.0 1,360,334 + puuR b1299 0.72 -0.6 1,360,413 - puuR b1299 0.86 +2.2 1,360,478 - +0.9 1,360,478 - +1.7 1,360,521 - -0.8 1,360,526 - -1.3 1,360,526 - +0.1 1,360,698 - +1.4 1,360,698 - -0.5 1,360,761 - -0.3 1,360,903 - -4.6 1,360,903 - -2.6 1,360,933 - puuC b1300 0.11 -0.7 1,361,146 + puuC b1300 0.25 -4.4 1,361,146 + puuC b1300 0.25 +1.2 1,361,262 - puuC b1300 0.33 -0.9 1,361,563 - puuC b1300 0.53 -1.7 1,361,674 - puuC b1300 0.61 -0.0 1,361,727 - puuC b1300 0.65 +1.2 1,362,160 + -0.1 1,362,229 - -0.3 1,362,317 + -2.9 1,362,520 + puuB b1301 0.21 -2.3 1,362,664 + puuB b1301 0.32 +0.8 1,362,716 - puuB b1301 0.36 +0.8 1,362,757 + puuB b1301 0.39 +0.2
Or see this region's nucleotide sequence