Experiment: PBS_cage_1_mice _Feces_22 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgL and nei are separated by 35 nucleotides nei and abrB overlap by 4 nucleotides abrB and ybgO are separated by 151 nucleotides
b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122
ybgL
b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949
nei
b0715: abrB - putative transport protein (VIMSS), at 745,946 to 746,992
abrB
b0716: ybgO - orf, hypothetical protein (VIMSS), at 747,144 to 748,205
ybgO
Position (kb) 745
746
747 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 744.978 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 745.140 kb on - strand at 745.148 kb on + strand at 745.194 kb on + strand at 745.194 kb on + strand at 745.286 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.358 kb on - strand, within nei at 745.358 kb on - strand, within nei at 745.419 kb on + strand, within nei at 745.689 kb on - strand, within nei at 745.991 kb on + strand at 746.028 kb on - strand at 746.035 kb on - strand at 746.088 kb on + strand, within abrB at 746.122 kb on - strand, within abrB at 746.218 kb on - strand, within abrB at 746.267 kb on - strand, within abrB at 746.335 kb on - strand, within abrB at 746.335 kb on - strand, within abrB at 746.412 kb on - strand, within abrB at 746.422 kb on - strand, within abrB at 746.509 kb on + strand, within abrB at 746.651 kb on - strand, within abrB at 746.768 kb on + strand, within abrB at 746.768 kb on + strand, within abrB at 746.815 kb on - strand, within abrB at 746.869 kb on + strand, within abrB at 746.929 kb on - strand at 747.003 kb on - strand at 747.003 kb on - strand at 747.024 kb on + strand at 747.027 kb on + strand at 747.042 kb on - strand at 747.172 kb on + strand at 747.172 kb on + strand at 747.251 kb on + strand, within ybgO at 747.251 kb on + strand, within ybgO at 747.255 kb on - strand, within ybgO at 747.255 kb on - strand, within ybgO at 747.303 kb on - strand, within ybgO at 747.329 kb on + strand, within ybgO at 747.346 kb on + strand, within ybgO at 747.363 kb on - strand, within ybgO at 747.363 kb on - strand, within ybgO at 747.372 kb on - strand, within ybgO at 747.372 kb on - strand, within ybgO at 747.382 kb on - strand, within ybgO at 747.382 kb on - strand, within ybgO at 747.393 kb on + strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.435 kb on + strand, within ybgO at 747.438 kb on - strand, within ybgO at 747.438 kb on - strand, within ybgO at 747.446 kb on + strand, within ybgO at 747.446 kb on + strand, within ybgO at 747.470 kb on - strand, within ybgO at 747.470 kb on - strand, within ybgO at 747.554 kb on + strand, within ybgO at 747.583 kb on + strand, within ybgO at 747.583 kb on + strand, within ybgO at 747.618 kb on - strand, within ybgO at 747.618 kb on - strand, within ybgO at 747.619 kb on + strand, within ybgO at 747.716 kb on + strand, within ybgO at 747.724 kb on - strand, within ybgO at 747.724 kb on - strand, within ybgO at 747.760 kb on + strand, within ybgO at 747.760 kb on + strand, within ybgO at 747.804 kb on - strand, within ybgO at 747.806 kb on + strand, within ybgO at 747.814 kb on - strand, within ybgO at 747.819 kb on - strand, within ybgO at 747.819 kb on - strand, within ybgO at 747.824 kb on - strand, within ybgO at 747.837 kb on + strand, within ybgO at 747.838 kb on + strand, within ybgO at 747.838 kb on + strand, within ybgO at 747.841 kb on + strand, within ybgO at 747.846 kb on - strand, within ybgO at 747.897 kb on + strand, within ybgO at 747.897 kb on + strand at 747.957 kb on + strand, within ybgO at 747.957 kb on + strand, within ybgO at 747.957 kb on + strand, within ybgO at 747.958 kb on + strand, within ybgO at 747.960 kb on - strand, within ybgO
Per-strain Table
Position Strand Gene LocusTag Fraction PBS_cage_1_mice _Feces_22 hr remove 744,978 - ybgL b0713 0.80 +1.8 744,978 - ybgL b0713 0.80 -0.2 745,140 - -4.2 745,148 + -4.6 745,194 + +0.6 745,194 + +0.1 745,286 + nei b0714 0.16 -1.8 745,297 + nei b0714 0.18 +2.1 745,297 + nei b0714 0.18 +0.1 745,358 - nei b0714 0.25 -0.7 745,358 - nei b0714 0.25 +0.5 745,419 + nei b0714 0.33 +1.4 745,689 - nei b0714 0.67 -3.6 745,991 + +0.7 746,028 - -0.6 746,035 - +0.3 746,088 + abrB b0715 0.14 +1.4 746,122 - abrB b0715 0.17 +0.0 746,218 - abrB b0715 0.26 +1.2 746,267 - abrB b0715 0.31 +0.7 746,335 - abrB b0715 0.37 +1.2 746,335 - abrB b0715 0.37 +1.9 746,412 - abrB b0715 0.45 +2.1 746,422 - abrB b0715 0.45 +1.2 746,509 + abrB b0715 0.54 -0.7 746,651 - abrB b0715 0.67 +0.8 746,768 + abrB b0715 0.79 -0.1 746,768 + abrB b0715 0.79 +0.7 746,815 - abrB b0715 0.83 -1.0 746,869 + abrB b0715 0.88 -0.4 746,929 - +1.0 747,003 - -1.4 747,003 - -1.6 747,024 + -0.2 747,027 + +1.9 747,042 - -1.4 747,172 + +4.9 747,172 + -1.0 747,251 + ybgO b0716 0.10 +3.2 747,251 + ybgO b0716 0.10 -2.1 747,255 - ybgO b0716 0.10 +0.5 747,255 - ybgO b0716 0.10 +0.5 747,303 - ybgO b0716 0.15 +1.9 747,329 + ybgO b0716 0.17 -1.3 747,346 + ybgO b0716 0.19 +0.5 747,363 - ybgO b0716 0.21 +1.6 747,363 - ybgO b0716 0.21 +0.6 747,372 - ybgO b0716 0.21 +1.6 747,372 - ybgO b0716 0.21 +2.9 747,382 - ybgO b0716 0.22 +1.0 747,382 - ybgO b0716 0.22 -4.8 747,393 + ybgO b0716 0.23 -0.2 747,393 - ybgO b0716 0.23 +1.2 747,393 - ybgO b0716 0.23 -2.0 747,393 - ybgO b0716 0.23 -2.9 747,435 + ybgO b0716 0.27 +2.0 747,438 - ybgO b0716 0.28 -2.2 747,438 - ybgO b0716 0.28 +0.5 747,446 + ybgO b0716 0.28 +1.6 747,446 + ybgO b0716 0.28 +0.2 747,470 - ybgO b0716 0.31 -0.2 747,470 - ybgO b0716 0.31 -0.1 747,554 + ybgO b0716 0.39 +1.8 747,583 + ybgO b0716 0.41 +0.9 747,583 + ybgO b0716 0.41 +0.1 747,618 - ybgO b0716 0.45 -4.1 747,618 - ybgO b0716 0.45 -2.2 747,619 + ybgO b0716 0.45 +0.5 747,716 + ybgO b0716 0.54 +0.1 747,724 - ybgO b0716 0.55 +0.1 747,724 - ybgO b0716 0.55 -0.1 747,760 + ybgO b0716 0.58 +0.5 747,760 + ybgO b0716 0.58 +0.1 747,804 - ybgO b0716 0.62 +0.6 747,806 + ybgO b0716 0.62 +0.4 747,814 - ybgO b0716 0.63 +1.2 747,819 - ybgO b0716 0.64 -1.4 747,819 - ybgO b0716 0.64 -0.4 747,824 - ybgO b0716 0.64 -1.8 747,837 + ybgO b0716 0.65 -0.5 747,838 + ybgO b0716 0.65 -1.0 747,838 + ybgO b0716 0.65 -2.9 747,841 + ybgO b0716 0.66 +0.8 747,846 - ybgO b0716 0.66 -1.1 747,897 + ybgO b0716 0.71 -2.7 747,897 + +0.0 747,957 + ybgO b0716 0.77 +2.5 747,957 + ybgO b0716 0.77 +1.0 747,957 + ybgO b0716 0.77 +2.3 747,958 + ybgO b0716 0.77 -3.1 747,960 - ybgO b0716 0.77 +0.4
Or see this region's nucleotide sequence