Strain Fitness in Escherichia coli BW25113 around b3328

Experiment: PBS_cage_1_mice _Feces_16 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspF and gspG are separated by 9 nucleotidesgspG and gspH are separated by 7 nucleotidesgspH and yheH overlap by 4 nucleotidesyheH and gspJ overlap by 8 nucleotides b3327: gspF - general secretory pathway component, cryptic (NCBI), at 3,457,839 to 3,459,035 gspF b3328: gspG - pseudopilin, cryptic, general secretion pathway (NCBI), at 3,459,045 to 3,459,482 gspG b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999 gspH b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373 yheH b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953 gspJ Position (kb) 3459 3460Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 3458.048 kb on + strand, within gspFat 3458.067 kb on + strand, within gspFat 3458.067 kb on + strand, within gspFat 3458.074 kb on - strand, within gspFat 3458.085 kb on + strand, within gspFat 3458.093 kb on - strand, within gspFat 3458.113 kb on - strand, within gspFat 3458.141 kb on + strand, within gspFat 3458.218 kb on + strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.243 kb on - strand, within gspFat 3458.243 kb on - strand, within gspFat 3458.324 kb on + strand, within gspFat 3458.324 kb on + strand, within gspFat 3458.324 kb on + strand, within gspFat 3458.338 kb on - strand, within gspFat 3458.338 kb on - strand, within gspFat 3458.348 kb on + strand, within gspFat 3458.348 kb on + strand, within gspFat 3458.353 kb on - strand, within gspFat 3458.358 kb on - strand, within gspFat 3458.406 kb on + strand, within gspFat 3458.420 kb on + strand, within gspFat 3458.420 kb on + strand, within gspFat 3458.428 kb on - strand, within gspFat 3458.453 kb on + strand, within gspFat 3458.475 kb on + strand, within gspFat 3458.505 kb on - strand, within gspFat 3458.521 kb on - strand, within gspFat 3458.521 kb on - strand, within gspFat 3458.632 kb on - strand, within gspFat 3458.632 kb on - strand, within gspFat 3458.638 kb on + strand, within gspFat 3458.663 kb on - strand, within gspFat 3458.668 kb on + strand, within gspFat 3458.671 kb on - strand, within gspFat 3458.687 kb on - strand, within gspFat 3458.687 kb on - strand, within gspFat 3458.689 kb on + strand, within gspFat 3458.711 kb on + strand, within gspFat 3458.817 kb on + strand, within gspFat 3458.817 kb on + strand, within gspFat 3458.822 kb on + strand, within gspFat 3458.822 kb on + strand, within gspFat 3458.823 kb on + strand, within gspFat 3458.878 kb on + strand, within gspFat 3458.889 kb on + strand, within gspFat 3458.899 kb on - strand, within gspFat 3458.909 kb on - strand, within gspFat 3458.913 kb on + strand, within gspFat 3458.920 kb on + strandat 3458.928 kb on - strandat 3458.936 kb on + strandat 3458.938 kb on - strandat 3458.949 kb on - strandat 3458.978 kb on + strandat 3458.980 kb on + strandat 3458.986 kb on - strandat 3459.001 kb on + strandat 3459.004 kb on - strandat 3459.015 kb on - strandat 3459.027 kb on - strandat 3459.078 kb on - strandat 3459.078 kb on - strandat 3459.203 kb on - strand, within gspGat 3459.203 kb on - strand, within gspGat 3459.226 kb on - strand, within gspGat 3459.244 kb on - strand, within gspGat 3459.252 kb on + strand, within gspGat 3459.252 kb on + strand, within gspGat 3459.288 kb on - strand, within gspGat 3459.288 kb on - strand, within gspGat 3459.320 kb on + strand, within gspGat 3459.320 kb on + strand, within gspGat 3459.324 kb on + strand, within gspGat 3459.355 kb on - strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.368 kb on - strand, within gspGat 3459.371 kb on + strand, within gspGat 3459.389 kb on - strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.399 kb on - strand, within gspGat 3459.421 kb on + strand, within gspGat 3459.446 kb on - strandat 3459.460 kb on - strandat 3459.460 kb on - strandat 3459.519 kb on - strandat 3459.519 kb on - strandat 3459.530 kb on - strandat 3459.530 kb on - strandat 3459.540 kb on - strandat 3459.570 kb on + strand, within gspHat 3459.578 kb on - strand, within gspHat 3459.603 kb on - strand, within gspHat 3459.607 kb on - strand, within gspHat 3459.607 kb on - strand, within gspHat 3459.674 kb on - strand, within gspHat 3459.698 kb on - strand, within gspHat 3459.716 kb on + strand, within gspHat 3459.742 kb on + strand, within gspHat 3459.742 kb on + strand, within gspHat 3459.747 kb on + strand, within gspHat 3459.818 kb on + strand, within gspHat 3459.851 kb on - strand, within gspHat 3459.851 kb on - strand, within gspHat 3459.893 kb on + strand, within gspHat 3459.920 kb on - strand, within gspHat 3459.930 kb on + strand, within gspHat 3459.937 kb on + strand, within gspHat 3459.937 kb on + strand, within gspHat 3459.947 kb on + strand, within gspHat 3459.947 kb on + strand, within gspHat 3459.955 kb on - strandat 3459.964 kb on - strandat 3459.978 kb on + strandat 3459.978 kb on + strandat 3459.978 kb on + strandat 3459.995 kb on + strandat 3460.012 kb on + strandat 3460.012 kb on + strandat 3460.031 kb on - strandat 3460.043 kb on - strand, within yheHat 3460.057 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.083 kb on + strand, within yheHat 3460.083 kb on + strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.089 kb on - strand, within yheHat 3460.089 kb on - strand, within yheHat 3460.107 kb on + strand, within yheHat 3460.114 kb on + strand, within yheHat 3460.133 kb on + strand, within yheHat 3460.198 kb on - strand, within yheHat 3460.198 kb on - strand, within yheHat 3460.224 kb on + strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.277 kb on - strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.385 kb on + strandat 3460.388 kb on - strandat 3460.395 kb on + strandat 3460.395 kb on + strandat 3460.401 kb on + strandat 3460.439 kb on - strand, within gspJ

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Per-strain Table

Position Strand Gene LocusTag Fraction PBS_cage_1_mice _Feces_16 hr
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3,458,048 + gspF b3327 0.17 +2.0
3,458,067 + gspF b3327 0.19 -1.2
3,458,067 + gspF b3327 0.19 -3.5
3,458,074 - gspF b3327 0.20 +2.2
3,458,085 + gspF b3327 0.21 +1.9
3,458,093 - gspF b3327 0.21 +1.4
3,458,113 - gspF b3327 0.23 -1.3
3,458,141 + gspF b3327 0.25 -1.6
3,458,218 + gspF b3327 0.32 +1.5
3,458,223 - gspF b3327 0.32 +2.6
3,458,223 - gspF b3327 0.32 -0.3
3,458,223 - gspF b3327 0.32 +3.6
3,458,223 - gspF b3327 0.32 -0.4
3,458,223 - gspF b3327 0.32 +2.9
3,458,223 - gspF b3327 0.32 +0.2
3,458,243 - gspF b3327 0.34 -1.7
3,458,243 - gspF b3327 0.34 +0.5
3,458,324 + gspF b3327 0.41 +2.4
3,458,324 + gspF b3327 0.41 +1.0
3,458,324 + gspF b3327 0.41 -1.1
3,458,338 - gspF b3327 0.42 -1.8
3,458,338 - gspF b3327 0.42 +2.1
3,458,348 + gspF b3327 0.43 +0.2
3,458,348 + gspF b3327 0.43 +3.5
3,458,353 - gspF b3327 0.43 -1.5
3,458,358 - gspF b3327 0.43 -4.9
3,458,406 + gspF b3327 0.47 -0.0
3,458,420 + gspF b3327 0.49 -0.0
3,458,420 + gspF b3327 0.49 +0.7
3,458,428 - gspF b3327 0.49 +0.3
3,458,453 + gspF b3327 0.51 -1.5
3,458,475 + gspF b3327 0.53 -1.5
3,458,505 - gspF b3327 0.56 -1.6
3,458,521 - gspF b3327 0.57 -0.5
3,458,521 - gspF b3327 0.57 -4.6
3,458,632 - gspF b3327 0.66 -0.1
3,458,632 - gspF b3327 0.66 +1.7
3,458,638 + gspF b3327 0.67 +1.5
3,458,663 - gspF b3327 0.69 -1.9
3,458,668 + gspF b3327 0.69 -0.3
3,458,671 - gspF b3327 0.70 +3.5
3,458,687 - gspF b3327 0.71 +0.2
3,458,687 - gspF b3327 0.71 -0.9
3,458,689 + gspF b3327 0.71 -0.1
3,458,711 + gspF b3327 0.73 +0.4
3,458,817 + gspF b3327 0.82 -0.0
3,458,817 + gspF b3327 0.82 -1.4
3,458,822 + gspF b3327 0.82 -1.8
3,458,822 + gspF b3327 0.82 +1.5
3,458,823 + gspF b3327 0.82 +0.0
3,458,878 + gspF b3327 0.87 -0.1
3,458,889 + gspF b3327 0.88 -0.6
3,458,899 - gspF b3327 0.89 +0.1
3,458,909 - gspF b3327 0.89 +2.0
3,458,913 + gspF b3327 0.90 -0.0
3,458,920 + +2.3
3,458,928 - +0.5
3,458,936 + -0.4
3,458,938 - -0.1
3,458,949 - -0.6
3,458,978 + +1.9
3,458,980 + -3.0
3,458,986 - +2.6
3,459,001 + -1.6
3,459,004 - +0.1
3,459,015 - +3.2
3,459,027 - +0.5
3,459,078 - +0.6
3,459,078 - +3.4
3,459,203 - gspG b3328 0.36 +0.2
3,459,203 - gspG b3328 0.36 -4.2
3,459,226 - gspG b3328 0.41 +3.3
3,459,244 - gspG b3328 0.45 +1.3
3,459,252 + gspG b3328 0.47 +3.6
3,459,252 + gspG b3328 0.47 -0.8
3,459,288 - gspG b3328 0.55 -2.0
3,459,288 - gspG b3328 0.55 +0.9
3,459,320 + gspG b3328 0.63 -2.1
3,459,320 + gspG b3328 0.63 +0.2
3,459,324 + gspG b3328 0.64 +2.0
3,459,355 - gspG b3328 0.71 -3.7
3,459,360 + gspG b3328 0.72 -0.6
3,459,360 + gspG b3328 0.72 -2.2
3,459,360 + gspG b3328 0.72 +0.7
3,459,360 + gspG b3328 0.72 +1.5
3,459,368 - gspG b3328 0.74 +0.5
3,459,371 + gspG b3328 0.74 -1.4
3,459,389 - gspG b3328 0.79 -1.5
3,459,391 + gspG b3328 0.79 -0.5
3,459,391 + gspG b3328 0.79 +1.4
3,459,391 + gspG b3328 0.79 +3.3
3,459,399 - gspG b3328 0.81 -1.3
3,459,421 + gspG b3328 0.86 +1.2
3,459,446 - +1.2
3,459,460 - +0.7
3,459,460 - +0.1
3,459,519 - -1.1
3,459,519 - -0.5
3,459,530 - -4.1
3,459,530 - -0.5
3,459,540 - +0.4
3,459,570 + gspH b3329 0.16 +0.9
3,459,578 - gspH b3329 0.17 +2.2
3,459,603 - gspH b3329 0.22 +2.9
3,459,607 - gspH b3329 0.23 +1.3
3,459,607 - gspH b3329 0.23 +0.7
3,459,674 - gspH b3329 0.36 +3.5
3,459,698 - gspH b3329 0.41 +0.2
3,459,716 + gspH b3329 0.44 -0.4
3,459,742 + gspH b3329 0.49 +0.7
3,459,742 + gspH b3329 0.49 -0.7
3,459,747 + gspH b3329 0.50 -2.1
3,459,818 + gspH b3329 0.64 +2.4
3,459,851 - gspH b3329 0.71 +0.8
3,459,851 - gspH b3329 0.71 +0.5
3,459,893 + gspH b3329 0.79 +1.7
3,459,920 - gspH b3329 0.84 +1.7
3,459,930 + gspH b3329 0.86 -5.7
3,459,937 + gspH b3329 0.88 -0.2
3,459,937 + gspH b3329 0.88 -1.2
3,459,947 + gspH b3329 0.90 +3.7
3,459,947 + gspH b3329 0.90 +0.6
3,459,955 - +2.6
3,459,964 - +1.6
3,459,978 + +0.9
3,459,978 + +0.3
3,459,978 + -0.3
3,459,995 + +1.0
3,460,012 + +1.2
3,460,012 + +0.4
3,460,031 - -1.2
3,460,043 - yheH b3330 0.12 +0.2
3,460,057 + yheH b3330 0.16 -0.2
3,460,081 + yheH b3330 0.22 -1.1
3,460,081 + yheH b3330 0.22 -0.1
3,460,081 + yheH b3330 0.22 +0.4
3,460,081 + yheH b3330 0.22 -0.9
3,460,083 + yheH b3330 0.23 -2.6
3,460,083 + yheH b3330 0.23 +0.4
3,460,086 - yheH b3330 0.24 -0.5
3,460,086 - yheH b3330 0.24 +0.8
3,460,086 - yheH b3330 0.24 +0.8
3,460,089 - yheH b3330 0.25 +0.6
3,460,089 - yheH b3330 0.25 +0.3
3,460,107 + yheH b3330 0.29 +2.2
3,460,114 + yheH b3330 0.31 +3.3
3,460,133 + yheH b3330 0.36 -0.1
3,460,198 - yheH b3330 0.53 +0.1
3,460,198 - yheH b3330 0.53 +2.1
3,460,224 + yheH b3330 0.60 +0.6
3,460,248 - yheH b3330 0.67 +2.6
3,460,248 - yheH b3330 0.67 +0.7
3,460,248 - yheH b3330 0.67 +2.5
3,460,277 - yheH b3330 0.74 +2.2
3,460,280 + yheH b3330 0.75 +1.2
3,460,280 + yheH b3330 0.75 -0.2
3,460,280 + yheH b3330 0.75 -2.4
3,460,280 + yheH b3330 0.75 -0.7
3,460,280 + yheH b3330 0.75 +1.0
3,460,385 + +2.2
3,460,388 - +2.3
3,460,395 + +0.5
3,460,395 + -0.6
3,460,401 + -1.5
3,460,439 - gspJ b3331 0.12 +2.7

Or see this region's nucleotide sequence