Strain Fitness in Escherichia coli BW25113 around b4460

Experiment: Phage_N4_cage_4_mouse_3_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntotsA and otsB overlap by 26 nucleotidesotsB and araH are separated by 166 nucleotidesaraH and araG are separated by 14 nucleotides b1896: otsA - trehalose-6-phosphate synthase (NCBI), at 1,978,212 to 1,979,636 otsA b1897: otsB - trehalose-6-phosphate phosphatase, biosynthetic (NCBI), at 1,979,611 to 1,980,411 otsB b4460: araH - fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq), at 1,980,578 to 1,981,564 araH b1900: araG - fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,981,579 to 1,983,093 araG Position (kb) 1980 1981 1982Strain fitness (log2 ratio) -1 0 1 2 3at 1979.748 kb on - strand, within otsBat 1979.885 kb on + strand, within otsBat 1979.998 kb on - strand, within otsBat 1980.189 kb on - strand, within otsBat 1980.229 kb on - strand, within otsBat 1980.256 kb on - strand, within otsBat 1980.274 kb on + strand, within otsBat 1980.341 kb on + strandat 1980.827 kb on - strandat 1980.984 kb on + strand, within araHat 1981.002 kb on - strand, within araHat 1981.002 kb on - strand, within araHat 1981.480 kb on + strandat 1981.487 kb on + strandat 1981.505 kb on - strandat 1981.531 kb on - strandat 1981.800 kb on + strand, within araGat 1982.173 kb on - strand, within araGat 1982.173 kb on - strand, within araGat 1982.180 kb on + strand, within araGat 1982.406 kb on + strand, within araG

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_4_mouse_3_Cecum_40 hr
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1,979,748 - otsB b1897 0.17 -0.4
1,979,885 + otsB b1897 0.34 +0.5
1,979,998 - otsB b1897 0.48 +1.2
1,980,189 - otsB b1897 0.72 +0.3
1,980,229 - otsB b1897 0.77 -0.3
1,980,256 - otsB b1897 0.81 -0.2
1,980,274 + otsB b1897 0.83 +0.2
1,980,341 + -0.0
1,980,827 - +3.2
1,980,984 + araH b4460 0.41 +1.5
1,981,002 - araH b4460 0.43 +1.6
1,981,002 - araH b4460 0.43 +3.2
1,981,480 + +2.1
1,981,487 + +1.9
1,981,505 - +1.9
1,981,531 - +2.0
1,981,800 + araG b1900 0.15 +1.9
1,982,173 - araG b1900 0.39 +2.0
1,982,173 - araG b1900 0.39 +2.5
1,982,180 + araG b1900 0.40 +1.4
1,982,406 + araG b1900 0.55 +2.1

Or see this region's nucleotide sequence