Strain Fitness in Escherichia coli BW25113 around b4214

Experiment: Phage_N4_cage_4_mouse_3_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpdB and cysQ are separated by 189 nucleotidescysQ and ytfI are separated by 211 nucleotides b4213: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (NCBI), at 4,432,645 to 4,434,588 cpdB b4214: cysQ - PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI), at 4,434,778 to 4,435,518 cysQ b4215: ytfI - hypothetical protein (NCBI), at 4,435,730 to 4,436,668 ytfI Position (kb) 4434 4435 4436Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4433.784 kb on - strand, within cpdBat 4433.819 kb on + strand, within cpdBat 4433.819 kb on + strand, within cpdBat 4433.884 kb on + strand, within cpdBat 4433.884 kb on + strand, within cpdBat 4433.885 kb on + strand, within cpdBat 4433.885 kb on + strand, within cpdBat 4433.885 kb on + strand, within cpdBat 4433.885 kb on + strand, within cpdBat 4433.912 kb on + strand, within cpdBat 4433.912 kb on + strand, within cpdBat 4433.913 kb on - strand, within cpdBat 4433.915 kb on - strand, within cpdBat 4433.923 kb on + strand, within cpdBat 4433.924 kb on - strand, within cpdBat 4433.924 kb on - strand, within cpdBat 4433.955 kb on + strand, within cpdBat 4433.978 kb on + strand, within cpdBat 4433.995 kb on - strand, within cpdBat 4433.995 kb on - strand, within cpdBat 4434.003 kb on + strand, within cpdBat 4434.022 kb on - strand, within cpdBat 4434.033 kb on - strand, within cpdBat 4434.061 kb on - strand, within cpdBat 4434.062 kb on - strand, within cpdBat 4434.102 kb on - strand, within cpdBat 4434.113 kb on - strand, within cpdBat 4434.136 kb on - strand, within cpdBat 4434.136 kb on - strand, within cpdBat 4434.137 kb on - strand, within cpdBat 4434.149 kb on + strand, within cpdBat 4434.149 kb on + strand, within cpdBat 4434.157 kb on - strand, within cpdBat 4434.225 kb on - strand, within cpdBat 4434.225 kb on - strand, within cpdBat 4434.235 kb on - strand, within cpdBat 4434.243 kb on - strand, within cpdBat 4434.263 kb on - strand, within cpdBat 4434.263 kb on - strand, within cpdBat 4434.268 kb on - strand, within cpdBat 4434.268 kb on - strand, within cpdBat 4434.273 kb on - strand, within cpdBat 4434.289 kb on - strand, within cpdBat 4434.291 kb on - strand, within cpdBat 4434.302 kb on - strand, within cpdBat 4434.344 kb on + strand, within cpdBat 4434.344 kb on + strand, within cpdBat 4434.344 kb on - strand, within cpdBat 4434.344 kb on - strand, within cpdBat 4434.357 kb on - strand, within cpdBat 4434.357 kb on - strand, within cpdBat 4434.357 kb on - strand, within cpdBat 4434.362 kb on - strand, within cpdBat 4434.383 kb on - strand, within cpdBat 4434.408 kb on - strandat 4434.410 kb on - strandat 4434.412 kb on + strandat 4434.425 kb on + strandat 4434.430 kb on - strandat 4434.430 kb on - strandat 4434.433 kb on - strandat 4434.433 kb on - strandat 4434.457 kb on + strandat 4434.457 kb on + strandat 4434.459 kb on + strandat 4434.480 kb on + strandat 4434.497 kb on + strandat 4434.497 kb on + strandat 4434.503 kb on + strandat 4434.524 kb on + strandat 4434.536 kb on - strandat 4434.536 kb on - strandat 4434.542 kb on - strandat 4434.544 kb on + strandat 4434.559 kb on + strandat 4434.559 kb on + strandat 4434.581 kb on - strandat 4434.605 kb on - strandat 4434.605 kb on - strandat 4434.652 kb on - strandat 4434.652 kb on - strandat 4434.654 kb on + strandat 4434.654 kb on - strandat 4434.661 kb on - strandat 4434.661 kb on - strandat 4434.698 kb on + strandat 4434.702 kb on - strandat 4434.704 kb on - strandat 4434.704 kb on - strandat 4434.706 kb on - strandat 4434.706 kb on - strandat 4434.710 kb on - strandat 4434.710 kb on - strandat 4434.724 kb on - strandat 4434.724 kb on - strandat 4434.726 kb on + strandat 4434.745 kb on + strandat 4434.762 kb on - strandat 4434.778 kb on - strandat 4434.782 kb on + strandat 4434.790 kb on + strandat 4434.807 kb on + strandat 4434.821 kb on + strandat 4434.826 kb on + strandat 4434.829 kb on - strandat 4434.829 kb on - strandat 4434.831 kb on + strandat 4434.832 kb on + strandat 4434.858 kb on - strand, within cysQat 4434.869 kb on - strand, within cysQat 4434.870 kb on + strand, within cysQat 4434.910 kb on - strand, within cysQat 4434.914 kb on + strand, within cysQat 4434.924 kb on - strand, within cysQat 4434.929 kb on + strand, within cysQat 4434.929 kb on + strand, within cysQat 4434.937 kb on - strand, within cysQat 4434.939 kb on + strand, within cysQat 4434.940 kb on - strand, within cysQat 4434.955 kb on - strand, within cysQat 4434.989 kb on - strand, within cysQat 4434.991 kb on - strand, within cysQat 4434.991 kb on - strand, within cysQat 4434.991 kb on - strand, within cysQat 4435.002 kb on - strand, within cysQat 4435.002 kb on - strand, within cysQat 4435.008 kb on + strand, within cysQat 4435.027 kb on - strand, within cysQat 4435.037 kb on + strand, within cysQat 4435.037 kb on - strand, within cysQat 4435.045 kb on - strand, within cysQat 4435.063 kb on + strand, within cysQat 4435.120 kb on + strand, within cysQat 4435.120 kb on + strand, within cysQat 4435.128 kb on - strand, within cysQat 4435.137 kb on - strand, within cysQat 4435.141 kb on + strand, within cysQat 4435.143 kb on + strand, within cysQat 4435.154 kb on - strand, within cysQat 4435.196 kb on + strand, within cysQat 4435.227 kb on + strand, within cysQat 4435.306 kb on + strand, within cysQat 4435.314 kb on - strand, within cysQat 4435.340 kb on + strand, within cysQat 4435.340 kb on + strand, within cysQat 4435.351 kb on + strand, within cysQat 4435.351 kb on + strand, within cysQat 4435.351 kb on + strand, within cysQat 4435.351 kb on + strand, within cysQat 4435.356 kb on + strand, within cysQat 4435.364 kb on - strand, within cysQat 4435.403 kb on + strand, within cysQat 4435.409 kb on + strand, within cysQat 4435.429 kb on + strand, within cysQat 4435.429 kb on - strand, within cysQat 4435.456 kb on + strandat 4435.461 kb on + strandat 4435.461 kb on + strandat 4435.461 kb on + strandat 4435.461 kb on + strandat 4435.462 kb on + strandat 4435.463 kb on + strandat 4435.463 kb on + strandat 4435.463 kb on + strandat 4435.463 kb on + strandat 4435.463 kb on + strandat 4435.463 kb on + strandat 4435.467 kb on + strandat 4435.471 kb on - strandat 4435.471 kb on - strandat 4435.482 kb on + strandat 4435.483 kb on + strandat 4435.490 kb on + strandat 4435.490 kb on - strandat 4435.496 kb on + strandat 4435.497 kb on + strandat 4435.497 kb on + strandat 4435.527 kb on + strandat 4435.528 kb on + strandat 4435.534 kb on + strandat 4435.535 kb on + strandat 4435.552 kb on + strandat 4436.395 kb on + strand, within ytfIat 4436.447 kb on + strand, within ytfIat 4436.483 kb on + strand, within ytfI

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_4_mouse_3_Cecum_40 hr
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4,433,784 - cpdB b4213 0.59 +0.2
4,433,819 + cpdB b4213 0.60 +1.2
4,433,819 + cpdB b4213 0.60 +1.0
4,433,884 + cpdB b4213 0.64 +0.6
4,433,884 + cpdB b4213 0.64 +0.3
4,433,885 + cpdB b4213 0.64 -0.5
4,433,885 + cpdB b4213 0.64 +0.0
4,433,885 + cpdB b4213 0.64 -0.1
4,433,885 + cpdB b4213 0.64 +0.1
4,433,912 + cpdB b4213 0.65 +0.0
4,433,912 + cpdB b4213 0.65 +1.0
4,433,913 - cpdB b4213 0.65 -0.2
4,433,915 - cpdB b4213 0.65 -0.4
4,433,923 + cpdB b4213 0.66 +0.8
4,433,924 - cpdB b4213 0.66 +0.2
4,433,924 - cpdB b4213 0.66 -1.7
4,433,955 + cpdB b4213 0.67 -0.5
4,433,978 + cpdB b4213 0.69 +0.8
4,433,995 - cpdB b4213 0.69 +0.2
4,433,995 - cpdB b4213 0.69 +1.0
4,434,003 + cpdB b4213 0.70 -1.0
4,434,022 - cpdB b4213 0.71 -1.1
4,434,033 - cpdB b4213 0.71 -2.6
4,434,061 - cpdB b4213 0.73 -0.1
4,434,062 - cpdB b4213 0.73 +0.1
4,434,102 - cpdB b4213 0.75 +0.8
4,434,113 - cpdB b4213 0.76 +0.5
4,434,136 - cpdB b4213 0.77 -1.3
4,434,136 - cpdB b4213 0.77 -0.1
4,434,137 - cpdB b4213 0.77 -0.3
4,434,149 + cpdB b4213 0.77 -0.8
4,434,149 + cpdB b4213 0.77 +1.3
4,434,157 - cpdB b4213 0.78 +0.5
4,434,225 - cpdB b4213 0.81 -0.1
4,434,225 - cpdB b4213 0.81 -3.3
4,434,235 - cpdB b4213 0.82 +0.1
4,434,243 - cpdB b4213 0.82 -0.6
4,434,263 - cpdB b4213 0.83 -0.9
4,434,263 - cpdB b4213 0.83 +0.4
4,434,268 - cpdB b4213 0.83 +1.9
4,434,268 - cpdB b4213 0.83 -1.6
4,434,273 - cpdB b4213 0.84 -1.9
4,434,289 - cpdB b4213 0.85 +0.1
4,434,291 - cpdB b4213 0.85 -0.8
4,434,302 - cpdB b4213 0.85 -0.2
4,434,344 + cpdB b4213 0.87 -0.2
4,434,344 + cpdB b4213 0.87 -0.4
4,434,344 - cpdB b4213 0.87 -0.1
4,434,344 - cpdB b4213 0.87 -1.0
4,434,357 - cpdB b4213 0.88 -0.2
4,434,357 - cpdB b4213 0.88 -0.6
4,434,357 - cpdB b4213 0.88 -1.3
4,434,362 - cpdB b4213 0.88 +0.2
4,434,383 - cpdB b4213 0.89 +0.5
4,434,408 - +0.4
4,434,410 - +0.6
4,434,412 + +0.2
4,434,425 + +0.5
4,434,430 - -1.6
4,434,430 - +0.5
4,434,433 - -0.4
4,434,433 - +0.5
4,434,457 + -0.8
4,434,457 + -0.2
4,434,459 + +0.2
4,434,480 + -0.0
4,434,497 + +0.1
4,434,497 + -0.1
4,434,503 + +0.6
4,434,524 + +1.0
4,434,536 - +0.7
4,434,536 - -1.5
4,434,542 - -0.2
4,434,544 + -0.3
4,434,559 + +0.5
4,434,559 + -0.9
4,434,581 - +0.2
4,434,605 - +0.2
4,434,605 - -0.5
4,434,652 - +0.3
4,434,652 - +1.3
4,434,654 + -0.1
4,434,654 - +0.1
4,434,661 - +0.8
4,434,661 - +0.6
4,434,698 + +0.4
4,434,702 - +0.2
4,434,704 - +0.2
4,434,704 - -0.5
4,434,706 - +0.2
4,434,706 - +0.1
4,434,710 - +0.9
4,434,710 - +0.9
4,434,724 - -0.2
4,434,724 - -0.9
4,434,726 + -0.5
4,434,745 + -0.5
4,434,762 - +0.1
4,434,778 - -1.7
4,434,782 + +0.3
4,434,790 + +0.0
4,434,807 + -0.2
4,434,821 + +0.4
4,434,826 + +0.2
4,434,829 - +2.1
4,434,829 - +0.5
4,434,831 + -0.0
4,434,832 + -0.1
4,434,858 - cysQ b4214 0.11 +0.3
4,434,869 - cysQ b4214 0.12 +0.3
4,434,870 + cysQ b4214 0.12 -0.4
4,434,910 - cysQ b4214 0.18 -0.4
4,434,914 + cysQ b4214 0.18 +0.4
4,434,924 - cysQ b4214 0.20 +0.5
4,434,929 + cysQ b4214 0.20 +0.1
4,434,929 + cysQ b4214 0.20 +0.3
4,434,937 - cysQ b4214 0.21 +0.2
4,434,939 + cysQ b4214 0.22 -0.2
4,434,940 - cysQ b4214 0.22 +0.4
4,434,955 - cysQ b4214 0.24 -0.0
4,434,989 - cysQ b4214 0.28 +0.0
4,434,991 - cysQ b4214 0.29 -0.3
4,434,991 - cysQ b4214 0.29 +0.6
4,434,991 - cysQ b4214 0.29 +0.3
4,435,002 - cysQ b4214 0.30 +0.3
4,435,002 - cysQ b4214 0.30 -0.4
4,435,008 + cysQ b4214 0.31 -1.8
4,435,027 - cysQ b4214 0.34 -0.3
4,435,037 + cysQ b4214 0.35 -0.2
4,435,037 - cysQ b4214 0.35 -2.7
4,435,045 - cysQ b4214 0.36 +0.2
4,435,063 + cysQ b4214 0.38 -0.2
4,435,120 + cysQ b4214 0.46 +0.6
4,435,120 + cysQ b4214 0.46 +1.5
4,435,128 - cysQ b4214 0.47 +1.3
4,435,137 - cysQ b4214 0.48 +3.0
4,435,141 + cysQ b4214 0.49 -0.8
4,435,143 + cysQ b4214 0.49 +0.3
4,435,154 - cysQ b4214 0.51 +0.2
4,435,196 + cysQ b4214 0.56 -0.0
4,435,227 + cysQ b4214 0.61 -0.1
4,435,306 + cysQ b4214 0.71 +0.7
4,435,314 - cysQ b4214 0.72 -0.6
4,435,340 + cysQ b4214 0.76 -0.5
4,435,340 + cysQ b4214 0.76 -0.5
4,435,351 + cysQ b4214 0.77 +0.7
4,435,351 + cysQ b4214 0.77 -0.4
4,435,351 + cysQ b4214 0.77 +0.0
4,435,351 + cysQ b4214 0.77 -0.7
4,435,356 + cysQ b4214 0.78 +0.7
4,435,364 - cysQ b4214 0.79 -0.0
4,435,403 + cysQ b4214 0.84 -0.2
4,435,409 + cysQ b4214 0.85 +0.6
4,435,429 + cysQ b4214 0.88 +0.2
4,435,429 - cysQ b4214 0.88 +0.8
4,435,456 + +0.3
4,435,461 + -2.1
4,435,461 + +0.1
4,435,461 + +1.1
4,435,461 + -0.7
4,435,462 + +0.4
4,435,463 + -0.9
4,435,463 + -0.6
4,435,463 + +1.0
4,435,463 + -0.8
4,435,463 + +0.5
4,435,463 + +0.7
4,435,467 + +0.3
4,435,471 - -2.6
4,435,471 - -0.3
4,435,482 + +0.2
4,435,483 + +0.3
4,435,490 + -0.0
4,435,490 - +2.1
4,435,496 + -0.3
4,435,497 + -0.8
4,435,497 + -0.1
4,435,527 + -0.5
4,435,528 + -0.2
4,435,534 + -3.0
4,435,535 + -0.5
4,435,552 + -2.8
4,436,395 + ytfI b4215 0.71 +2.1
4,436,447 + ytfI b4215 0.76 -0.7
4,436,483 + ytfI b4215 0.80 +2.1

Or see this region's nucleotide sequence