Strain Fitness in Escherichia coli BW25113 around b0687

Experiment: Phage_N4_cage_4_mouse_3_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybfF and seqA are separated by 184 nucleotidesseqA and pgm are separated by 25 nucleotides b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025 ybfF b0687: seqA - regulatory protein for replication initiation (NCBI), at 712,210 to 712,755 seqA b0688: pgm - phosphoglucomutase (NCBI), at 712,781 to 714,421 pgm Position (kb) 712 713Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 711.400 kb on - strand, within ybfFat 711.569 kb on + strand, within ybfFat 711.668 kb on - strand, within ybfFat 711.685 kb on + strand, within ybfFat 711.728 kb on - strand, within ybfFat 711.730 kb on - strand, within ybfFat 711.730 kb on - strand, within ybfFat 711.803 kb on - strand, within ybfFat 711.803 kb on - strand, within ybfFat 711.900 kb on - strand, within ybfFat 711.923 kb on + strand, within ybfFat 711.923 kb on + strand, within ybfFat 711.933 kb on + strand, within ybfFat 712.060 kb on + strandat 712.077 kb on + strandat 712.124 kb on + strandat 712.202 kb on + strandat 712.758 kb on - strandat 712.814 kb on - strandat 712.937 kb on + strandat 712.978 kb on + strand, within pgmat 713.023 kb on + strand, within pgmat 713.081 kb on + strand, within pgmat 713.205 kb on + strand, within pgmat 713.428 kb on - strand, within pgmat 713.435 kb on + strand, within pgmat 713.435 kb on + strand, within pgmat 713.525 kb on - strand, within pgmat 713.580 kb on + strand, within pgmat 713.655 kb on - strand, within pgm

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_4_mouse_3_Cecum_40 hr
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711,400 - ybfF b0686 0.18 +0.0
711,569 + ybfF b0686 0.40 -0.2
711,668 - ybfF b0686 0.53 +0.1
711,685 + ybfF b0686 0.55 -0.4
711,728 - ybfF b0686 0.61 +0.3
711,730 - ybfF b0686 0.61 +0.6
711,730 - ybfF b0686 0.61 +0.5
711,803 - ybfF b0686 0.71 +0.6
711,803 - ybfF b0686 0.71 +0.7
711,900 - ybfF b0686 0.84 -0.5
711,923 + ybfF b0686 0.87 -0.4
711,923 + ybfF b0686 0.87 -0.6
711,933 + ybfF b0686 0.88 +0.1
712,060 + +0.4
712,077 + -0.1
712,124 + +1.0
712,202 + +0.3
712,758 - -0.0
712,814 - -3.7
712,937 + -3.3
712,978 + pgm b0688 0.12 -2.7
713,023 + pgm b0688 0.15 -4.8
713,081 + pgm b0688 0.18 -3.6
713,205 + pgm b0688 0.26 -4.3
713,428 - pgm b0688 0.39 -0.9
713,435 + pgm b0688 0.40 -3.8
713,435 + pgm b0688 0.40 -2.2
713,525 - pgm b0688 0.45 -4.3
713,580 + pgm b0688 0.49 -4.4
713,655 - pgm b0688 0.53 -2.7

Or see this region's nucleotide sequence