Experiment: Phage_N4_cage_4_mouse_1_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfbQ and yfbR are separated by 83 nucleotides yfbR and yfbS are separated by 58 nucleotides yfbS and yfbT are separated by 86 nucleotides yfbT and b2294 are separated by 10 nucleotides
b2290: yfbQ - aspartate aminotransferase (NCBI), at 2,405,583 to 2,406,800
yfbQ
b2291: yfbR - hypothetical protein (NCBI), at 2,406,884 to 2,407,483
yfbR
b2292: yfbS - predicted transporter (NCBI), at 2,407,542 to 2,409,374
yfbS
b2293: yfbT - putative phosphatase (VIMSS), at 2,409,461 to 2,410,111
yfbT
b2294: b2294 - orf, hypothetical protein (VIMSS), at 2,410,122 to 2,410,616
b2294
Position (kb)
2407
2408
2409
2410 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2406.584 kb on - strand, within yfbQ at 2406.584 kb on - strand, within yfbQ at 2406.684 kb on + strand at 2406.692 kb on - strand at 2406.692 kb on - strand at 2406.692 kb on - strand at 2406.692 kb on - strand at 2406.726 kb on - strand at 2406.865 kb on + strand at 2407.100 kb on - strand, within yfbR at 2407.167 kb on - strand, within yfbR at 2407.230 kb on - strand, within yfbR at 2407.256 kb on - strand, within yfbR at 2407.466 kb on - strand at 2407.483 kb on - strand at 2407.483 kb on - strand at 2407.485 kb on - strand at 2407.485 kb on - strand at 2407.558 kb on + strand at 2407.677 kb on - strand at 2407.677 kb on - strand at 2407.769 kb on - strand, within yfbS at 2407.775 kb on + strand, within yfbS at 2407.777 kb on + strand, within yfbS at 2407.806 kb on - strand, within yfbS at 2407.812 kb on - strand, within yfbS at 2407.812 kb on - strand, within yfbS at 2408.014 kb on - strand, within yfbS at 2408.073 kb on + strand, within yfbS at 2408.126 kb on + strand, within yfbS at 2408.145 kb on - strand, within yfbS at 2408.164 kb on - strand, within yfbS at 2408.166 kb on + strand, within yfbS at 2408.191 kb on + strand, within yfbS at 2408.252 kb on + strand, within yfbS at 2408.252 kb on + strand, within yfbS at 2408.275 kb on + strand, within yfbS at 2408.400 kb on + strand, within yfbS at 2408.408 kb on - strand, within yfbS at 2408.408 kb on - strand, within yfbS at 2408.413 kb on - strand, within yfbS at 2408.494 kb on + strand, within yfbS at 2408.519 kb on - strand, within yfbS at 2408.593 kb on - strand, within yfbS at 2408.736 kb on + strand, within yfbS at 2408.764 kb on - strand, within yfbS at 2408.775 kb on - strand, within yfbS at 2408.818 kb on + strand, within yfbS at 2408.818 kb on + strand, within yfbS at 2408.911 kb on + strand, within yfbS at 2408.911 kb on + strand, within yfbS at 2408.932 kb on - strand, within yfbS at 2409.178 kb on + strand, within yfbS at 2409.178 kb on + strand, within yfbS at 2409.195 kb on + strand at 2409.195 kb on + strand at 2409.208 kb on - strand at 2409.251 kb on - strand at 2409.258 kb on - strand at 2409.513 kb on + strand at 2409.636 kb on + strand, within yfbT at 2409.641 kb on + strand, within yfbT at 2409.913 kb on - strand, within yfbT at 2410.159 kb on + strand at 2410.161 kb on - strand at 2410.287 kb on - strand, within b2294 at 2410.358 kb on + strand, within b2294 at 2410.358 kb on + strand, within b2294
Per-strain Table
Position Strand Gene LocusTag Fraction Phage_N4_cage_4_mouse_1_Cecum_40 hr remove 2,406,584 - yfbQ b2290 0.82 -1.2 2,406,584 - yfbQ b2290 0.82 +0.5 2,406,684 + +0.6 2,406,692 - -1.2 2,406,692 - +2.6 2,406,692 - -1.2 2,406,692 - -0.0 2,406,726 - +0.3 2,406,865 + +2.4 2,407,100 - yfbR b2291 0.36 -0.3 2,407,167 - yfbR b2291 0.47 +0.6 2,407,230 - yfbR b2291 0.58 +0.0 2,407,256 - yfbR b2291 0.62 +0.1 2,407,466 - -0.2 2,407,483 - +1.4 2,407,483 - +1.1 2,407,485 - +0.1 2,407,485 - +0.2 2,407,558 + +0.9 2,407,677 - -5.9 2,407,677 - +0.3 2,407,769 - yfbS b2292 0.12 +0.4 2,407,775 + yfbS b2292 0.13 -0.0 2,407,777 + yfbS b2292 0.13 -3.9 2,407,806 - yfbS b2292 0.14 -0.2 2,407,812 - yfbS b2292 0.15 -1.4 2,407,812 - yfbS b2292 0.15 -0.1 2,408,014 - yfbS b2292 0.26 +0.1 2,408,073 + yfbS b2292 0.29 +0.3 2,408,126 + yfbS b2292 0.32 +0.8 2,408,145 - yfbS b2292 0.33 -0.1 2,408,164 - yfbS b2292 0.34 +0.7 2,408,166 + yfbS b2292 0.34 -0.1 2,408,191 + yfbS b2292 0.35 +1.5 2,408,252 + yfbS b2292 0.39 +1.5 2,408,252 + yfbS b2292 0.39 -0.9 2,408,275 + yfbS b2292 0.40 +0.6 2,408,400 + yfbS b2292 0.47 +0.2 2,408,408 - yfbS b2292 0.47 +0.5 2,408,408 - yfbS b2292 0.47 +0.5 2,408,413 - yfbS b2292 0.48 +1.5 2,408,494 + yfbS b2292 0.52 +0.5 2,408,519 - yfbS b2292 0.53 +1.2 2,408,593 - yfbS b2292 0.57 +0.9 2,408,736 + yfbS b2292 0.65 +0.4 2,408,764 - yfbS b2292 0.67 -0.6 2,408,775 - yfbS b2292 0.67 +1.8 2,408,818 + yfbS b2292 0.70 +0.0 2,408,818 + yfbS b2292 0.70 +3.0 2,408,911 + yfbS b2292 0.75 +0.7 2,408,911 + yfbS b2292 0.75 +1.5 2,408,932 - yfbS b2292 0.76 +0.7 2,409,178 + yfbS b2292 0.89 +0.0 2,409,178 + yfbS b2292 0.89 -0.1 2,409,195 + +0.7 2,409,195 + +1.9 2,409,208 - +0.6 2,409,251 - -0.4 2,409,258 - +0.4 2,409,513 + -0.0 2,409,636 + yfbT b2293 0.27 +0.0 2,409,641 + yfbT b2293 0.28 +1.0 2,409,913 - yfbT b2293 0.69 -0.8 2,410,159 + +0.6 2,410,161 - -0.1 2,410,287 - b2294 b2294 0.33 +0.1 2,410,358 + b2294 b2294 0.48 -3.0 2,410,358 + b2294 b2294 0.48 +0.1
Or see this region's nucleotide sequence