Strain Fitness in Escherichia coli BW25113 around b4418

Experiment: Phage_N4_cage_3_mouse_4_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrluC and yceF are separated by 111 nucleotidesyceF and sraB overlap by 7 nucleotidessraB and yceD are separated by 36 nucleotidesyceD and rpmF are separated by 51 nucleotidesrpmF and plsX are separated by 80 nucleotides b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122 rluC b1087: yceF - orf, hypothetical protein (VIMSS), at 1,145,234 to 1,145,818 yceF b4418: sraB - unknown RNA (NCBI), at 1,145,812 to 1,145,980 sraB b1088: yceD - hypothetical protein (NCBI), at 1,146,017 to 1,146,538 yceD b1089: rpmF - 50S ribosomal protein L32 (NCBI), at 1,146,590 to 1,146,763 rpmF b1090: plsX - glycerolphosphate auxotrophy in plsB background (VIMSS), at 1,146,844 to 1,147,914 plsX Position (kb) 1145 1146Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1144.867 kb on + strand, within rluCat 1144.867 kb on + strand, within rluCat 1144.899 kb on + strand, within rluCat 1144.988 kb on + strand, within rluCat 1144.988 kb on + strand, within rluCat 1144.998 kb on + strand, within rluCat 1144.998 kb on + strand, within rluCat 1145.011 kb on - strand, within rluCat 1145.011 kb on - strand, within rluCat 1145.035 kb on + strandat 1145.058 kb on - strandat 1145.145 kb on - strandat 1145.279 kb on - strandat 1145.381 kb on + strand, within yceFat 1145.381 kb on + strand, within yceFat 1145.381 kb on + strand, within yceFat 1145.381 kb on + strand, within yceFat 1145.389 kb on - strand, within yceFat 1145.389 kb on - strand, within yceFat 1145.389 kb on - strand, within yceFat 1145.395 kb on + strand, within yceFat 1145.511 kb on - strand, within yceFat 1145.516 kb on - strand, within yceFat 1145.516 kb on - strand, within yceFat 1145.576 kb on - strand, within yceFat 1145.610 kb on - strand, within yceFat 1145.614 kb on - strand, within yceFat 1145.673 kb on - strand, within yceFat 1145.724 kb on - strand, within yceFat 1145.734 kb on - strand, within yceFat 1145.734 kb on - strand, within yceFat 1145.841 kb on + strand, within sraBat 1145.847 kb on - strand, within sraBat 1146.042 kb on + strandat 1146.052 kb on + strandat 1146.096 kb on + strand, within yceDat 1146.096 kb on + strand, within yceDat 1146.117 kb on + strand, within yceDat 1146.265 kb on + strand, within yceDat 1146.265 kb on + strand, within yceDat 1146.265 kb on + strand, within yceDat 1146.265 kb on + strand, within yceDat 1146.277 kb on + strand, within yceDat 1146.277 kb on + strand, within yceDat 1146.310 kb on + strand, within yceDat 1146.373 kb on + strand, within yceDat 1146.373 kb on + strand, within yceD

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_4_Cecum_40 hr
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1,144,867 + rluC b1086 0.73 -3.1
1,144,867 + rluC b1086 0.73 -2.5
1,144,899 + rluC b1086 0.77 +1.3
1,144,988 + rluC b1086 0.86 -2.2
1,144,988 + rluC b1086 0.86 -1.3
1,144,998 + rluC b1086 0.87 -4.7
1,144,998 + rluC b1086 0.87 -4.9
1,145,011 - rluC b1086 0.88 -0.2
1,145,011 - rluC b1086 0.88 -3.5
1,145,035 + +1.6
1,145,058 - +1.5
1,145,145 - +1.6
1,145,279 - +0.7
1,145,381 + yceF b1087 0.25 +0.0
1,145,381 + yceF b1087 0.25 +0.4
1,145,381 + yceF b1087 0.25 +1.1
1,145,381 + yceF b1087 0.25 +0.5
1,145,389 - yceF b1087 0.26 -0.8
1,145,389 - yceF b1087 0.26 -0.0
1,145,389 - yceF b1087 0.26 -2.9
1,145,395 + yceF b1087 0.28 +1.0
1,145,511 - yceF b1087 0.47 -2.9
1,145,516 - yceF b1087 0.48 +1.8
1,145,516 - yceF b1087 0.48 -1.0
1,145,576 - yceF b1087 0.58 +1.0
1,145,610 - yceF b1087 0.64 +1.0
1,145,614 - yceF b1087 0.65 -0.6
1,145,673 - yceF b1087 0.75 +0.7
1,145,724 - yceF b1087 0.84 +0.6
1,145,734 - yceF b1087 0.85 -3.4
1,145,734 - yceF b1087 0.85 +1.3
1,145,841 + sraB b4418 0.17 -2.1
1,145,847 - sraB b4418 0.21 -0.6
1,146,042 + -4.2
1,146,052 + -2.2
1,146,096 + yceD b1088 0.15 -2.4
1,146,096 + yceD b1088 0.15 -4.2
1,146,117 + yceD b1088 0.19 -2.2
1,146,265 + yceD b1088 0.48 +2.1
1,146,265 + yceD b1088 0.48 -4.9
1,146,265 + yceD b1088 0.48 -1.9
1,146,265 + yceD b1088 0.48 +0.1
1,146,277 + yceD b1088 0.50 -3.3
1,146,277 + yceD b1088 0.50 -0.6
1,146,310 + yceD b1088 0.56 -0.4
1,146,373 + yceD b1088 0.68 -4.1
1,146,373 + yceD b1088 0.68 +0.5

Or see this region's nucleotide sequence