Strain Fitness in Escherichia coli BW25113 around b4259

Experiment: Phage_N4_cage_3_mouse_4_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntvalS and holC overlap by 1 nucleotidesholC and pepA are separated by 159 nucleotides b4258: valS - valyl-tRNA synthetase (NCBI), at 4,479,005 to 4,481,860 valS b4259: holC - DNA polymerase III subunit chi (NCBI), at 4,481,860 to 4,482,303 holC b4260: pepA - leucyl aminopeptidase (NCBI), at 4,482,463 to 4,483,974 pepA Position (kb) 4481 4482 4483Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4482.341 kb on + strandat 4482.349 kb on - strandat 4482.354 kb on - strandat 4482.369 kb on - strandat 4482.369 kb on - strandat 4482.379 kb on - strandat 4482.394 kb on + strandat 4482.394 kb on - strandat 4482.402 kb on - strandat 4482.402 kb on - strandat 4482.404 kb on + strandat 4482.445 kb on + strandat 4482.452 kb on + strandat 4482.458 kb on + strandat 4482.460 kb on + strandat 4482.494 kb on + strandat 4482.582 kb on - strandat 4482.650 kb on + strand, within pepAat 4482.690 kb on - strand, within pepAat 4482.698 kb on - strand, within pepAat 4482.706 kb on + strandat 4482.706 kb on + strandat 4482.726 kb on - strand, within pepAat 4482.741 kb on + strand, within pepAat 4482.749 kb on - strand, within pepAat 4482.749 kb on - strand, within pepAat 4482.749 kb on - strand, within pepAat 4482.787 kb on + strand, within pepAat 4482.787 kb on + strand, within pepAat 4482.800 kb on - strand, within pepAat 4482.805 kb on - strand, within pepAat 4482.822 kb on + strand, within pepAat 4482.822 kb on + strand, within pepAat 4482.822 kb on + strand, within pepAat 4482.822 kb on + strand, within pepAat 4482.883 kb on + strand, within pepAat 4482.900 kb on + strand, within pepAat 4482.900 kb on + strand, within pepAat 4482.900 kb on + strand, within pepAat 4482.900 kb on + strand, within pepAat 4482.900 kb on + strand, within pepAat 4482.908 kb on - strand, within pepAat 4482.920 kb on + strand, within pepAat 4482.959 kb on - strand, within pepAat 4482.964 kb on - strand, within pepAat 4482.964 kb on - strand, within pepAat 4482.977 kb on + strand, within pepAat 4482.977 kb on + strand, within pepAat 4482.985 kb on - strand, within pepAat 4483.056 kb on - strand, within pepAat 4483.056 kb on - strand, within pepAat 4483.122 kb on + strand, within pepAat 4483.150 kb on + strand, within pepAat 4483.158 kb on - strand, within pepAat 4483.169 kb on - strand, within pepAat 4483.179 kb on + strand, within pepA

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_4_Cecum_40 hr
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4,482,341 + -2.4
4,482,349 - -1.0
4,482,354 - -0.2
4,482,369 - +1.2
4,482,369 - -3.8
4,482,379 - -1.0
4,482,394 + -5.3
4,482,394 - -1.2
4,482,402 - +1.0
4,482,402 - +0.1
4,482,404 + -1.3
4,482,445 + -3.8
4,482,452 + -2.0
4,482,458 + -0.2
4,482,460 + -1.2
4,482,494 + -1.2
4,482,582 - -0.5
4,482,650 + pepA b4260 0.12 +0.4
4,482,690 - pepA b4260 0.15 -0.8
4,482,698 - pepA b4260 0.16 -0.2
4,482,706 + -0.5
4,482,706 + -1.1
4,482,726 - pepA b4260 0.17 -1.9
4,482,741 + pepA b4260 0.18 -0.5
4,482,749 - pepA b4260 0.19 -0.5
4,482,749 - pepA b4260 0.19 +0.4
4,482,749 - pepA b4260 0.19 -0.3
4,482,787 + pepA b4260 0.21 -2.7
4,482,787 + pepA b4260 0.21 +1.3
4,482,800 - pepA b4260 0.22 -2.3
4,482,805 - pepA b4260 0.23 +0.3
4,482,822 + pepA b4260 0.24 -4.1
4,482,822 + pepA b4260 0.24 +0.4
4,482,822 + pepA b4260 0.24 +0.2
4,482,822 + pepA b4260 0.24 -2.5
4,482,883 + pepA b4260 0.28 -3.6
4,482,900 + pepA b4260 0.29 +0.1
4,482,900 + pepA b4260 0.29 -4.3
4,482,900 + pepA b4260 0.29 -4.9
4,482,900 + pepA b4260 0.29 -0.1
4,482,900 + pepA b4260 0.29 -3.5
4,482,908 - pepA b4260 0.29 +0.1
4,482,920 + pepA b4260 0.30 -5.5
4,482,959 - pepA b4260 0.33 +1.1
4,482,964 - pepA b4260 0.33 +1.6
4,482,964 - pepA b4260 0.33 +0.4
4,482,977 + pepA b4260 0.34 -3.5
4,482,977 + pepA b4260 0.34 +1.0
4,482,985 - pepA b4260 0.35 -6.2
4,483,056 - pepA b4260 0.39 +0.5
4,483,056 - pepA b4260 0.39 -0.0
4,483,122 + pepA b4260 0.44 -6.1
4,483,150 + pepA b4260 0.45 -4.8
4,483,158 - pepA b4260 0.46 -0.2
4,483,169 - pepA b4260 0.47 -1.0
4,483,179 + pepA b4260 0.47 +0.5

Or see this region's nucleotide sequence