Strain Fitness in Escherichia coli BW25113 around b1100

Experiment: Phage_N4_cage_3_mouse_4_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntholB and ycfH are separated by 10 nucleotidesycfH and ptsG are separated by 294 nucleotides b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989 holB b1100: ycfH - predicted metallodependent hydrolase (NCBI), at 1,156,000 to 1,156,797 ycfH b1101: ptsG - fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI), at 1,157,092 to 1,158,525 ptsG Position (kb) 1155 1156 1157Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1155.056 kb on + strandat 1155.960 kb on + strandat 1155.960 kb on + strandat 1155.966 kb on + strandat 1156.034 kb on - strandat 1156.056 kb on + strandat 1156.056 kb on + strandat 1156.065 kb on + strandat 1156.078 kb on - strandat 1156.157 kb on + strand, within ycfHat 1156.157 kb on + strand, within ycfHat 1156.258 kb on - strand, within ycfHat 1156.318 kb on - strand, within ycfHat 1156.318 kb on - strand, within ycfHat 1156.358 kb on + strand, within ycfHat 1156.358 kb on + strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.456 kb on + strand, within ycfHat 1156.484 kb on - strand, within ycfHat 1156.513 kb on - strand, within ycfHat 1156.513 kb on - strand, within ycfHat 1156.568 kb on + strand, within ycfHat 1156.568 kb on + strand, within ycfHat 1156.598 kb on - strand, within ycfHat 1156.598 kb on - strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.617 kb on + strand, within ycfHat 1156.622 kb on + strand, within ycfHat 1156.636 kb on - strand, within ycfHat 1156.636 kb on - strand, within ycfHat 1156.686 kb on + strand, within ycfHat 1156.692 kb on - strand, within ycfHat 1156.696 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.731 kb on + strandat 1157.020 kb on + strandat 1157.020 kb on + strandat 1157.020 kb on - strandat 1157.027 kb on + strandat 1157.072 kb on + strandat 1157.073 kb on + strandat 1157.080 kb on - strandat 1157.080 kb on - strandat 1157.152 kb on + strandat 1157.216 kb on + strandat 1157.216 kb on + strandat 1157.237 kb on - strand, within ptsGat 1157.288 kb on + strand, within ptsGat 1157.313 kb on - strand, within ptsGat 1157.313 kb on - strand, within ptsGat 1157.440 kb on + strand, within ptsGat 1157.440 kb on + strand, within ptsGat 1157.454 kb on + strand, within ptsGat 1157.454 kb on + strand, within ptsGat 1157.509 kb on - strand, within ptsGat 1157.509 kb on - strand, within ptsGat 1157.558 kb on + strand, within ptsGat 1157.625 kb on + strand, within ptsGat 1157.629 kb on - strand, within ptsGat 1157.629 kb on - strand, within ptsGat 1157.644 kb on - strand, within ptsGat 1157.650 kb on + strand, within ptsGat 1157.651 kb on - strand, within ptsGat 1157.671 kb on - strand, within ptsGat 1157.727 kb on - strand, within ptsGat 1157.761 kb on + strand, within ptsG

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_4_Cecum_40 hr
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1,155,056 + -4.2
1,155,960 + -2.3
1,155,960 + +1.8
1,155,966 + +2.1
1,156,034 - +0.0
1,156,056 + -1.6
1,156,056 + +0.7
1,156,065 + +0.4
1,156,078 - -1.0
1,156,157 + ycfH b1100 0.20 -4.9
1,156,157 + ycfH b1100 0.20 -4.2
1,156,258 - ycfH b1100 0.32 +0.8
1,156,318 - ycfH b1100 0.40 -1.3
1,156,318 - ycfH b1100 0.40 -5.0
1,156,358 + ycfH b1100 0.45 -5.1
1,156,358 + ycfH b1100 0.45 +0.8
1,156,392 - ycfH b1100 0.49 +0.2
1,156,392 - ycfH b1100 0.49 -3.0
1,156,392 - ycfH b1100 0.49 -0.0
1,156,392 - ycfH b1100 0.49 -3.8
1,156,456 + ycfH b1100 0.57 +0.9
1,156,484 - ycfH b1100 0.61 +0.1
1,156,513 - ycfH b1100 0.64 -1.9
1,156,513 - ycfH b1100 0.64 -0.5
1,156,568 + ycfH b1100 0.71 +0.3
1,156,568 + ycfH b1100 0.71 -1.0
1,156,598 - ycfH b1100 0.75 +0.0
1,156,598 - ycfH b1100 0.75 -3.8
1,156,602 + ycfH b1100 0.75 +1.0
1,156,602 + ycfH b1100 0.75 +1.4
1,156,602 + ycfH b1100 0.75 -0.8
1,156,617 + ycfH b1100 0.77 -0.2
1,156,622 + ycfH b1100 0.78 -0.2
1,156,636 - ycfH b1100 0.80 -1.3
1,156,636 - ycfH b1100 0.80 -2.3
1,156,686 + ycfH b1100 0.86 -1.0
1,156,692 - ycfH b1100 0.87 -4.3
1,156,696 + ycfH b1100 0.87 -0.5
1,156,698 + ycfH b1100 0.87 -0.1
1,156,698 + ycfH b1100 0.87 +0.4
1,156,698 + ycfH b1100 0.87 +1.4
1,156,731 + +0.1
1,157,020 + -0.5
1,157,020 + -4.7
1,157,020 - +0.5
1,157,027 + -2.0
1,157,072 + -0.7
1,157,073 + -0.4
1,157,080 - -2.3
1,157,080 - -2.6
1,157,152 + -0.8
1,157,216 + -3.1
1,157,216 + -4.1
1,157,237 - ptsG b1101 0.10 -1.1
1,157,288 + ptsG b1101 0.14 +1.9
1,157,313 - ptsG b1101 0.15 +0.3
1,157,313 - ptsG b1101 0.15 -1.2
1,157,440 + ptsG b1101 0.24 +0.1
1,157,440 + ptsG b1101 0.24 +0.8
1,157,454 + ptsG b1101 0.25 +1.0
1,157,454 + ptsG b1101 0.25 +1.0
1,157,509 - ptsG b1101 0.29 +0.8
1,157,509 - ptsG b1101 0.29 -3.6
1,157,558 + ptsG b1101 0.32 -1.9
1,157,625 + ptsG b1101 0.37 +0.3
1,157,629 - ptsG b1101 0.37 -2.5
1,157,629 - ptsG b1101 0.37 -4.0
1,157,644 - ptsG b1101 0.38 +0.5
1,157,650 + ptsG b1101 0.39 +1.1
1,157,651 - ptsG b1101 0.39 -4.1
1,157,671 - ptsG b1101 0.40 -5.1
1,157,727 - ptsG b1101 0.44 -3.8
1,157,761 + ptsG b1101 0.47 -5.3

Or see this region's nucleotide sequence