Strain Fitness in Escherichia coli BW25113 around b4147

Experiment: Phage_N4_cage_3_mouse_3_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjeK and efp are separated by 41 nucleotidesefp and ecnA are separated by 51 nucleotidesecnA and ecnB are separated by 110 nucleotidesecnB and sugE are separated by 175 nucleotidessugE and blc overlap by 4 nucleotides b4146: yjeK - predicted lysine aminomutase (NCBI), at 4,372,652 to 4,373,680 yjeK b4147: efp - elongation factor P (NCBI), at 4,373,722 to 4,374,288 efp b4410: ecnA - entericidin A membrane lipoprotein, antidote entericidin B (NCBI), at 4,374,340 to 4,374,465 ecnA b4411: ecnB - entericidin B membrane lipoprotein (NCBI), at 4,374,576 to 4,374,722 ecnB b4148: sugE - suppresses groEL, may be chaperone (VIMSS), at 4,374,898 to 4,375,215 sugE b4149: blc - outer membrane lipoprotein (lipocalin) (NCBI), at 4,375,212 to 4,375,745 blc Position (kb) 4373 4374 4375Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 4372.731 kb on - strandat 4372.731 kb on - strandat 4372.801 kb on - strand, within yjeKat 4372.801 kb on - strand, within yjeKat 4372.874 kb on - strand, within yjeKat 4372.876 kb on - strand, within yjeKat 4372.912 kb on + strand, within yjeKat 4372.915 kb on - strand, within yjeKat 4373.029 kb on + strand, within yjeKat 4373.029 kb on + strand, within yjeKat 4373.140 kb on - strand, within yjeKat 4373.140 kb on - strand, within yjeKat 4373.221 kb on + strand, within yjeKat 4373.255 kb on + strand, within yjeKat 4373.313 kb on - strand, within yjeKat 4373.387 kb on + strand, within yjeKat 4373.609 kb on + strandat 4373.609 kb on + strandat 4373.609 kb on + strandat 4373.695 kb on - strandat 4374.270 kb on + strandat 4374.303 kb on + strandat 4374.303 kb on + strandat 4374.336 kb on + strandat 4374.401 kb on - strand, within ecnAat 4374.646 kb on - strand, within ecnBat 4374.668 kb on - strand, within ecnBat 4374.668 kb on - strand, within ecnBat 4374.869 kb on - strandat 4374.869 kb on - strandat 4374.900 kb on + strandat 4374.900 kb on + strandat 4374.908 kb on - strandat 4374.909 kb on - strandat 4374.909 kb on - strandat 4374.938 kb on - strand, within sugEat 4374.939 kb on + strand, within sugEat 4374.948 kb on - strand, within sugEat 4375.022 kb on - strand, within sugEat 4375.030 kb on + strand, within sugEat 4375.072 kb on - strand, within sugEat 4375.073 kb on + strand, within sugEat 4375.118 kb on + strand, within sugEat 4375.210 kb on + strandat 4375.210 kb on + strandat 4375.216 kb on + strandat 4375.218 kb on - strandat 4375.218 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_3_Cecum_40 hr
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4,372,731 - -1.6
4,372,731 - -1.0
4,372,801 - yjeK b4146 0.14 -0.6
4,372,801 - yjeK b4146 0.14 -1.7
4,372,874 - yjeK b4146 0.22 -1.0
4,372,876 - yjeK b4146 0.22 -2.4
4,372,912 + yjeK b4146 0.25 -1.6
4,372,915 - yjeK b4146 0.26 -1.7
4,373,029 + yjeK b4146 0.37 -0.6
4,373,029 + yjeK b4146 0.37 -1.7
4,373,140 - yjeK b4146 0.47 -1.4
4,373,140 - yjeK b4146 0.47 -1.7
4,373,221 + yjeK b4146 0.55 -1.8
4,373,255 + yjeK b4146 0.59 -1.7
4,373,313 - yjeK b4146 0.64 +1.6
4,373,387 + yjeK b4146 0.71 -4.3
4,373,609 + -1.7
4,373,609 + -0.7
4,373,609 + +2.3
4,373,695 - -4.7
4,374,270 + -1.5
4,374,303 + +0.9
4,374,303 + -0.2
4,374,336 + -0.5
4,374,401 - ecnA b4410 0.48 +1.2
4,374,646 - ecnB b4411 0.48 -1.7
4,374,668 - ecnB b4411 0.63 -0.5
4,374,668 - ecnB b4411 0.63 +1.0
4,374,869 - +1.6
4,374,869 - +0.5
4,374,900 + +1.4
4,374,900 + +0.4
4,374,908 - -0.9
4,374,909 - -3.4
4,374,909 - -0.5
4,374,938 - sugE b4148 0.13 +1.6
4,374,939 + sugE b4148 0.13 -5.1
4,374,948 - sugE b4148 0.16 +1.8
4,375,022 - sugE b4148 0.39 -0.2
4,375,030 + sugE b4148 0.42 +0.4
4,375,072 - sugE b4148 0.55 -0.3
4,375,073 + sugE b4148 0.55 +0.8
4,375,118 + sugE b4148 0.69 +1.2
4,375,210 + -4.6
4,375,210 + -4.6
4,375,216 + +1.2
4,375,218 - +1.3
4,375,218 - +0.9

Or see this region's nucleotide sequence