Experiment: Phage_N4_cage_3_mouse_3_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjeK and efp are separated by 41 nucleotides efp and ecnA are separated by 51 nucleotides ecnA and ecnB are separated by 110 nucleotides ecnB and sugE are separated by 175 nucleotides sugE and blc overlap by 4 nucleotides
b4146: yjeK - predicted lysine aminomutase (NCBI), at 4,372,652 to 4,373,680
yjeK
b4147: efp - elongation factor P (NCBI), at 4,373,722 to 4,374,288
efp
b4410: ecnA - entericidin A membrane lipoprotein, antidote entericidin B (NCBI), at 4,374,340 to 4,374,465
ecnA
b4411: ecnB - entericidin B membrane lipoprotein (NCBI), at 4,374,576 to 4,374,722
ecnB
b4148: sugE - suppresses groEL, may be chaperone (VIMSS), at 4,374,898 to 4,375,215
sugE
b4149: blc - outer membrane lipoprotein (lipocalin) (NCBI), at 4,375,212 to 4,375,745
blc
Position (kb)
4373
4374
4375 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4372.731 kb on - strand at 4372.731 kb on - strand at 4372.801 kb on - strand, within yjeK at 4372.801 kb on - strand, within yjeK at 4372.874 kb on - strand, within yjeK at 4372.876 kb on - strand, within yjeK at 4372.912 kb on + strand, within yjeK at 4372.915 kb on - strand, within yjeK at 4373.029 kb on + strand, within yjeK at 4373.029 kb on + strand, within yjeK at 4373.140 kb on - strand, within yjeK at 4373.140 kb on - strand, within yjeK at 4373.221 kb on + strand, within yjeK at 4373.255 kb on + strand, within yjeK at 4373.313 kb on - strand, within yjeK at 4373.387 kb on + strand, within yjeK at 4373.609 kb on + strand at 4373.609 kb on + strand at 4373.609 kb on + strand at 4373.695 kb on - strand at 4374.270 kb on + strand at 4374.303 kb on + strand at 4374.303 kb on + strand at 4374.336 kb on + strand at 4374.401 kb on - strand, within ecnA at 4374.646 kb on - strand, within ecnB at 4374.668 kb on - strand, within ecnB at 4374.668 kb on - strand, within ecnB at 4374.869 kb on - strand at 4374.869 kb on - strand at 4374.900 kb on + strand at 4374.900 kb on + strand at 4374.908 kb on - strand at 4374.909 kb on - strand at 4374.909 kb on - strand at 4374.938 kb on - strand, within sugE at 4374.939 kb on + strand, within sugE at 4374.948 kb on - strand, within sugE at 4375.022 kb on - strand, within sugE at 4375.030 kb on + strand, within sugE at 4375.072 kb on - strand, within sugE at 4375.073 kb on + strand, within sugE at 4375.118 kb on + strand, within sugE at 4375.210 kb on + strand at 4375.210 kb on + strand at 4375.216 kb on + strand at 4375.218 kb on - strand at 4375.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_3_Cecum_40 hr remove 4,372,731 - -1.6 4,372,731 - -1.0 4,372,801 - yjeK b4146 0.14 -0.6 4,372,801 - yjeK b4146 0.14 -1.7 4,372,874 - yjeK b4146 0.22 -1.0 4,372,876 - yjeK b4146 0.22 -2.4 4,372,912 + yjeK b4146 0.25 -1.6 4,372,915 - yjeK b4146 0.26 -1.7 4,373,029 + yjeK b4146 0.37 -0.6 4,373,029 + yjeK b4146 0.37 -1.7 4,373,140 - yjeK b4146 0.47 -1.4 4,373,140 - yjeK b4146 0.47 -1.7 4,373,221 + yjeK b4146 0.55 -1.8 4,373,255 + yjeK b4146 0.59 -1.7 4,373,313 - yjeK b4146 0.64 +1.6 4,373,387 + yjeK b4146 0.71 -4.3 4,373,609 + -1.7 4,373,609 + -0.7 4,373,609 + +2.3 4,373,695 - -4.7 4,374,270 + -1.5 4,374,303 + +0.9 4,374,303 + -0.2 4,374,336 + -0.5 4,374,401 - ecnA b4410 0.48 +1.2 4,374,646 - ecnB b4411 0.48 -1.7 4,374,668 - ecnB b4411 0.63 -0.5 4,374,668 - ecnB b4411 0.63 +1.0 4,374,869 - +1.6 4,374,869 - +0.5 4,374,900 + +1.4 4,374,900 + +0.4 4,374,908 - -0.9 4,374,909 - -3.4 4,374,909 - -0.5 4,374,938 - sugE b4148 0.13 +1.6 4,374,939 + sugE b4148 0.13 -5.1 4,374,948 - sugE b4148 0.16 +1.8 4,375,022 - sugE b4148 0.39 -0.2 4,375,030 + sugE b4148 0.42 +0.4 4,375,072 - sugE b4148 0.55 -0.3 4,375,073 + sugE b4148 0.55 +0.8 4,375,118 + sugE b4148 0.69 +1.2 4,375,210 + -4.6 4,375,210 + -4.6 4,375,216 + +1.2 4,375,218 - +1.3 4,375,218 - +0.9
Or see this region's nucleotide sequence