Strain Fitness in Escherichia coli BW25113 around b3329

Experiment: Phage_N4_cage_3_mouse_3_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspF and gspG are separated by 9 nucleotidesgspG and gspH are separated by 7 nucleotidesgspH and yheH overlap by 4 nucleotidesyheH and gspJ overlap by 8 nucleotidesgspJ and gspK overlap by 8 nucleotides b3327: gspF - general secretory pathway component, cryptic (NCBI), at 3,457,839 to 3,459,035 gspF b3328: gspG - pseudopilin, cryptic, general secretion pathway (NCBI), at 3,459,045 to 3,459,482 gspG b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999 gspH b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373 yheH b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953 gspJ b3332: gspK - general secretory pathway component, cryptic (NCBI), at 3,460,946 to 3,461,929 gspK Position (kb) 3459 3460Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3458.505 kb on - strand, within gspFat 3458.521 kb on - strand, within gspFat 3458.521 kb on - strand, within gspFat 3458.632 kb on - strand, within gspFat 3458.632 kb on - strand, within gspFat 3458.638 kb on + strand, within gspFat 3458.663 kb on - strand, within gspFat 3458.668 kb on + strand, within gspFat 3458.671 kb on - strand, within gspFat 3458.687 kb on - strand, within gspFat 3458.687 kb on - strand, within gspFat 3458.689 kb on + strand, within gspFat 3458.711 kb on + strand, within gspFat 3458.817 kb on + strand, within gspFat 3458.817 kb on + strand, within gspFat 3458.822 kb on + strand, within gspFat 3458.822 kb on + strand, within gspFat 3458.823 kb on + strand, within gspFat 3458.878 kb on + strand, within gspFat 3458.889 kb on + strand, within gspFat 3458.899 kb on - strand, within gspFat 3458.909 kb on - strand, within gspFat 3458.913 kb on + strand, within gspFat 3458.920 kb on + strandat 3458.928 kb on - strandat 3458.936 kb on + strandat 3458.938 kb on - strandat 3458.949 kb on - strandat 3458.978 kb on + strandat 3458.980 kb on + strandat 3458.986 kb on - strandat 3459.001 kb on + strandat 3459.004 kb on - strandat 3459.015 kb on - strandat 3459.027 kb on - strandat 3459.078 kb on - strandat 3459.078 kb on - strandat 3459.203 kb on - strand, within gspGat 3459.203 kb on - strand, within gspGat 3459.226 kb on - strand, within gspGat 3459.244 kb on - strand, within gspGat 3459.252 kb on + strand, within gspGat 3459.252 kb on + strand, within gspGat 3459.288 kb on - strand, within gspGat 3459.288 kb on - strand, within gspGat 3459.320 kb on + strand, within gspGat 3459.320 kb on + strand, within gspGat 3459.324 kb on + strand, within gspGat 3459.355 kb on - strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.368 kb on - strand, within gspGat 3459.371 kb on + strand, within gspGat 3459.389 kb on - strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.399 kb on - strand, within gspGat 3459.421 kb on + strand, within gspGat 3459.446 kb on - strandat 3459.460 kb on - strandat 3459.460 kb on - strandat 3459.519 kb on - strandat 3459.519 kb on - strandat 3459.530 kb on - strandat 3459.530 kb on - strandat 3459.540 kb on - strandat 3459.570 kb on + strand, within gspHat 3459.578 kb on - strand, within gspHat 3459.603 kb on - strand, within gspHat 3459.607 kb on - strand, within gspHat 3459.607 kb on - strand, within gspHat 3459.674 kb on - strand, within gspHat 3459.698 kb on - strand, within gspHat 3459.716 kb on + strand, within gspHat 3459.742 kb on + strand, within gspHat 3459.742 kb on + strand, within gspHat 3459.747 kb on + strand, within gspHat 3459.818 kb on + strand, within gspHat 3459.851 kb on - strand, within gspHat 3459.851 kb on - strand, within gspHat 3459.893 kb on + strand, within gspHat 3459.920 kb on - strand, within gspHat 3459.930 kb on + strand, within gspHat 3459.937 kb on + strand, within gspHat 3459.937 kb on + strand, within gspHat 3459.947 kb on + strand, within gspHat 3459.947 kb on + strand, within gspHat 3459.955 kb on - strandat 3459.964 kb on - strandat 3459.978 kb on + strandat 3459.978 kb on + strandat 3459.978 kb on + strandat 3459.995 kb on + strandat 3460.012 kb on + strandat 3460.012 kb on + strandat 3460.031 kb on - strandat 3460.043 kb on - strand, within yheHat 3460.057 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.083 kb on + strand, within yheHat 3460.083 kb on + strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.089 kb on - strand, within yheHat 3460.089 kb on - strand, within yheHat 3460.107 kb on + strand, within yheHat 3460.114 kb on + strand, within yheHat 3460.133 kb on + strand, within yheHat 3460.198 kb on - strand, within yheHat 3460.198 kb on - strand, within yheHat 3460.224 kb on + strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.277 kb on - strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.385 kb on + strandat 3460.388 kb on - strandat 3460.395 kb on + strandat 3460.395 kb on + strandat 3460.401 kb on + strandat 3460.439 kb on - strand, within gspJat 3460.518 kb on + strandat 3460.524 kb on + strand, within gspJat 3460.529 kb on - strand, within gspJat 3460.534 kb on - strand, within gspJat 3460.538 kb on - strand, within gspJat 3460.569 kb on - strand, within gspJat 3460.569 kb on - strand, within gspJat 3460.580 kb on - strand, within gspJat 3460.592 kb on - strand, within gspJat 3460.596 kb on + strand, within gspJat 3460.639 kb on - strand, within gspJat 3460.666 kb on + strandat 3460.667 kb on + strand, within gspJat 3460.668 kb on - strand, within gspJat 3460.674 kb on - strand, within gspJat 3460.686 kb on - strand, within gspJat 3460.686 kb on - strand, within gspJat 3460.705 kb on - strand, within gspJat 3460.705 kb on - strand, within gspJat 3460.707 kb on + strand, within gspJat 3460.713 kb on - strand, within gspJat 3460.713 kb on - strand, within gspJat 3460.725 kb on - strand, within gspJat 3460.725 kb on - strand, within gspJat 3460.727 kb on - strand, within gspJat 3460.732 kb on - strand, within gspJat 3460.734 kb on - strand, within gspJat 3460.770 kb on - strand, within gspJat 3460.770 kb on - strand, within gspJat 3460.770 kb on - strand, within gspJat 3460.771 kb on - strand, within gspJat 3460.835 kb on + strand, within gspJat 3460.835 kb on + strand, within gspJat 3460.848 kb on + strand, within gspJat 3460.848 kb on + strand, within gspJat 3460.854 kb on + strand, within gspJat 3460.870 kb on - strand, within gspJat 3460.872 kb on - strand, within gspJat 3460.872 kb on - strand, within gspJat 3460.877 kb on + strand, within gspJat 3460.890 kb on - strand, within gspJat 3460.900 kb on + strandat 3460.900 kb on + strandat 3460.937 kb on - strandat 3460.937 kb on - strandat 3460.978 kb on - strandat 3460.978 kb on - strandat 3460.982 kb on + strandat 3460.982 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_3_Cecum_40 hr
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3,458,505 - gspF b3327 0.56 -1.6
3,458,521 - gspF b3327 0.57 -4.7
3,458,521 - gspF b3327 0.57 -5.0
3,458,632 - gspF b3327 0.66 +0.4
3,458,632 - gspF b3327 0.66 +1.6
3,458,638 + gspF b3327 0.67 +0.4
3,458,663 - gspF b3327 0.69 -2.0
3,458,668 + gspF b3327 0.69 +1.0
3,458,671 - gspF b3327 0.70 -0.2
3,458,687 - gspF b3327 0.71 -2.4
3,458,687 - gspF b3327 0.71 +1.1
3,458,689 + gspF b3327 0.71 -0.1
3,458,711 + gspF b3327 0.73 -4.0
3,458,817 + gspF b3327 0.82 +0.8
3,458,817 + gspF b3327 0.82 +1.1
3,458,822 + gspF b3327 0.82 +2.5
3,458,822 + gspF b3327 0.82 -3.8
3,458,823 + gspF b3327 0.82 +0.9
3,458,878 + gspF b3327 0.87 -0.2
3,458,889 + gspF b3327 0.88 +2.2
3,458,899 - gspF b3327 0.89 -0.4
3,458,909 - gspF b3327 0.89 +0.7
3,458,913 + gspF b3327 0.90 +0.5
3,458,920 + -0.5
3,458,928 - +1.2
3,458,936 + -1.6
3,458,938 - +0.4
3,458,949 - -4.3
3,458,978 + -0.7
3,458,980 + -3.4
3,458,986 - +0.1
3,459,001 + +1.6
3,459,004 - +1.3
3,459,015 - +1.8
3,459,027 - -0.2
3,459,078 - +0.3
3,459,078 - +0.6
3,459,203 - gspG b3328 0.36 +0.5
3,459,203 - gspG b3328 0.36 -0.9
3,459,226 - gspG b3328 0.41 -2.7
3,459,244 - gspG b3328 0.45 -1.0
3,459,252 + gspG b3328 0.47 +0.1
3,459,252 + gspG b3328 0.47 -0.3
3,459,288 - gspG b3328 0.55 -1.8
3,459,288 - gspG b3328 0.55 -0.0
3,459,320 + gspG b3328 0.63 +1.8
3,459,320 + gspG b3328 0.63 +0.5
3,459,324 + gspG b3328 0.64 +0.1
3,459,355 - gspG b3328 0.71 +1.6
3,459,360 + gspG b3328 0.72 -4.5
3,459,360 + gspG b3328 0.72 +1.8
3,459,360 + gspG b3328 0.72 +0.6
3,459,360 + gspG b3328 0.72 +0.8
3,459,368 - gspG b3328 0.74 +0.2
3,459,371 + gspG b3328 0.74 -0.3
3,459,389 - gspG b3328 0.79 +0.8
3,459,391 + gspG b3328 0.79 -1.4
3,459,391 + gspG b3328 0.79 +1.6
3,459,391 + gspG b3328 0.79 +1.5
3,459,399 - gspG b3328 0.81 +2.5
3,459,421 + gspG b3328 0.86 -4.0
3,459,446 - +1.0
3,459,460 - -3.6
3,459,460 - -0.5
3,459,519 - +0.6
3,459,519 - +2.0
3,459,530 - -4.5
3,459,530 - +1.2
3,459,540 - +1.4
3,459,570 + gspH b3329 0.16 +0.2
3,459,578 - gspH b3329 0.17 -0.7
3,459,603 - gspH b3329 0.22 -0.5
3,459,607 - gspH b3329 0.23 +0.8
3,459,607 - gspH b3329 0.23 -1.0
3,459,674 - gspH b3329 0.36 -5.4
3,459,698 - gspH b3329 0.41 +0.4
3,459,716 + gspH b3329 0.44 +1.2
3,459,742 + gspH b3329 0.49 +0.8
3,459,742 + gspH b3329 0.49 -4.3
3,459,747 + gspH b3329 0.50 -1.1
3,459,818 + gspH b3329 0.64 +0.1
3,459,851 - gspH b3329 0.71 +0.2
3,459,851 - gspH b3329 0.71 +0.5
3,459,893 + gspH b3329 0.79 +0.2
3,459,920 - gspH b3329 0.84 +1.4
3,459,930 + gspH b3329 0.86 +0.6
3,459,937 + gspH b3329 0.88 +0.5
3,459,937 + gspH b3329 0.88 +0.6
3,459,947 + gspH b3329 0.90 -4.6
3,459,947 + gspH b3329 0.90 -1.4
3,459,955 - +1.0
3,459,964 - -5.1
3,459,978 + +2.7
3,459,978 + +1.0
3,459,978 + +0.0
3,459,995 + -2.1
3,460,012 + -0.5
3,460,012 + -4.2
3,460,031 - -0.5
3,460,043 - yheH b3330 0.12 +0.2
3,460,057 + yheH b3330 0.16 +2.7
3,460,081 + yheH b3330 0.22 +2.2
3,460,081 + yheH b3330 0.22 +0.0
3,460,081 + yheH b3330 0.22 +0.4
3,460,081 + yheH b3330 0.22 +0.5
3,460,083 + yheH b3330 0.23 -2.9
3,460,083 + yheH b3330 0.23 -5.4
3,460,086 - yheH b3330 0.24 -1.1
3,460,086 - yheH b3330 0.24 +1.3
3,460,086 - yheH b3330 0.24 +1.5
3,460,089 - yheH b3330 0.25 +1.0
3,460,089 - yheH b3330 0.25 +0.7
3,460,107 + yheH b3330 0.29 +1.8
3,460,114 + yheH b3330 0.31 +1.9
3,460,133 + yheH b3330 0.36 +0.1
3,460,198 - yheH b3330 0.53 +0.6
3,460,198 - yheH b3330 0.53 +1.4
3,460,224 + yheH b3330 0.60 +0.6
3,460,248 - yheH b3330 0.67 +0.3
3,460,248 - yheH b3330 0.67 +0.5
3,460,248 - yheH b3330 0.67 -0.4
3,460,277 - yheH b3330 0.74 -0.6
3,460,280 + yheH b3330 0.75 -0.9
3,460,280 + yheH b3330 0.75 +0.3
3,460,280 + yheH b3330 0.75 +1.7
3,460,280 + yheH b3330 0.75 -2.7
3,460,280 + yheH b3330 0.75 +0.3
3,460,385 + +2.0
3,460,388 - +1.6
3,460,395 + +0.6
3,460,395 + -1.0
3,460,401 + +0.9
3,460,439 - gspJ b3331 0.12 +1.5
3,460,518 + -1.5
3,460,524 + gspJ b3331 0.27 +0.0
3,460,529 - gspJ b3331 0.28 +0.9
3,460,534 - gspJ b3331 0.29 +1.3
3,460,538 - gspJ b3331 0.29 +0.5
3,460,569 - gspJ b3331 0.35 -0.2
3,460,569 - gspJ b3331 0.35 -3.9
3,460,580 - gspJ b3331 0.36 +1.3
3,460,592 - gspJ b3331 0.38 +2.0
3,460,596 + gspJ b3331 0.39 -5.4
3,460,639 - gspJ b3331 0.46 +1.4
3,460,666 + -1.3
3,460,667 + gspJ b3331 0.51 +1.3
3,460,668 - gspJ b3331 0.51 -1.4
3,460,674 - gspJ b3331 0.52 +0.9
3,460,686 - gspJ b3331 0.54 -0.8
3,460,686 - gspJ b3331 0.54 -0.0
3,460,705 - gspJ b3331 0.58 +1.8
3,460,705 - gspJ b3331 0.58 +0.3
3,460,707 + gspJ b3331 0.58 -4.1
3,460,713 - gspJ b3331 0.59 +0.2
3,460,713 - gspJ b3331 0.59 -0.1
3,460,725 - gspJ b3331 0.61 -1.6
3,460,725 - gspJ b3331 0.61 +1.6
3,460,727 - gspJ b3331 0.61 -0.4
3,460,732 - gspJ b3331 0.62 -1.2
3,460,734 - gspJ b3331 0.63 +1.7
3,460,770 - gspJ b3331 0.69 +0.7
3,460,770 - gspJ b3331 0.69 +0.5
3,460,770 - gspJ b3331 0.69 +0.8
3,460,771 - gspJ b3331 0.69 +1.0
3,460,835 + gspJ b3331 0.80 +0.9
3,460,835 + gspJ b3331 0.80 +2.0
3,460,848 + gspJ b3331 0.82 -4.0
3,460,848 + gspJ b3331 0.82 +0.8
3,460,854 + gspJ b3331 0.83 +1.0
3,460,870 - gspJ b3331 0.86 +0.5
3,460,872 - gspJ b3331 0.86 +1.1
3,460,872 - gspJ b3331 0.86 +1.5
3,460,877 + gspJ b3331 0.87 +1.3
3,460,890 - gspJ b3331 0.89 -0.2
3,460,900 + +1.6
3,460,900 + +2.2
3,460,937 - -1.9
3,460,937 - +0.5
3,460,978 - -3.0
3,460,978 - -3.8
3,460,982 + +2.1
3,460,982 + -1.4

Or see this region's nucleotide sequence