Strain Fitness in Escherichia coli BW25113 around b2581

Experiment: Phage_N4_cage_3_mouse_3_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntung and yfiF are separated by 47 nucleotidesyfiF and trxC are separated by 206 nucleotidestrxC and yfiP are separated by 68 nucleotides b2580: ung - uracil-DNA glycosylase (NCBI), at 2,714,776 to 2,715,465 ung b2581: yfiF - predicted methyltransferase (NCBI), at 2,715,513 to 2,716,550 yfiF b2582: trxC - thioredoxin 2 (NCBI), at 2,716,757 to 2,717,176 trxC b2583: yfiP - orf, hypothetical protein (VIMSS), at 2,717,245 to 2,717,943 yfiP Position (kb) 2715 2716 2717Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2714.557 kb on - strandat 2714.557 kb on - strandat 2714.652 kb on - strandat 2714.777 kb on - strandat 2714.937 kb on - strand, within ungat 2714.940 kb on + strand, within ungat 2714.978 kb on + strand, within ungat 2715.050 kb on + strand, within ungat 2715.089 kb on + strand, within ungat 2715.121 kb on + strand, within ungat 2715.161 kb on + strand, within ungat 2715.195 kb on - strand, within ungat 2715.311 kb on + strand, within ungat 2715.451 kb on + strandat 2715.608 kb on + strandat 2715.654 kb on + strand, within yfiFat 2715.706 kb on + strand, within yfiFat 2715.746 kb on - strand, within yfiFat 2715.854 kb on + strand, within yfiFat 2715.879 kb on - strand, within yfiFat 2715.997 kb on + strand, within yfiFat 2715.997 kb on + strand, within yfiFat 2715.997 kb on + strand, within yfiFat 2715.997 kb on + strand, within yfiFat 2716.005 kb on - strand, within yfiFat 2716.005 kb on - strand, within yfiFat 2716.005 kb on - strand, within yfiFat 2716.005 kb on - strand, within yfiFat 2716.141 kb on + strand, within yfiFat 2716.141 kb on + strandat 2716.183 kb on - strand, within yfiFat 2716.183 kb on - strand, within yfiFat 2716.229 kb on + strand, within yfiFat 2716.265 kb on - strand, within yfiFat 2716.265 kb on - strand, within yfiFat 2716.313 kb on + strand, within yfiFat 2716.313 kb on + strand, within yfiFat 2716.387 kb on - strand, within yfiFat 2716.387 kb on - strand, within yfiFat 2716.440 kb on + strand, within yfiFat 2716.446 kb on + strand, within yfiFat 2716.485 kb on + strandat 2716.742 kb on + strandat 2716.762 kb on - strandat 2716.832 kb on + strand, within trxCat 2717.016 kb on - strand, within trxCat 2717.103 kb on + strand, within trxCat 2717.387 kb on - strand, within yfiPat 2717.387 kb on - strand, within yfiPat 2717.445 kb on + strand, within yfiPat 2717.445 kb on + strand, within yfiPat 2717.458 kb on - strand, within yfiP

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_3_Cecum_40 hr
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2,714,557 - +0.6
2,714,557 - -3.3
2,714,652 - -0.0
2,714,777 - -0.2
2,714,937 - ung b2580 0.23 -4.5
2,714,940 + ung b2580 0.24 +0.7
2,714,978 + ung b2580 0.29 +0.4
2,715,050 + ung b2580 0.40 +0.6
2,715,089 + ung b2580 0.45 +0.4
2,715,121 + ung b2580 0.50 +0.6
2,715,161 + ung b2580 0.56 +0.0
2,715,195 - ung b2580 0.61 -1.1
2,715,311 + ung b2580 0.78 -1.3
2,715,451 + +1.6
2,715,608 + -0.9
2,715,654 + yfiF b2581 0.14 +1.4
2,715,706 + yfiF b2581 0.19 +0.7
2,715,746 - yfiF b2581 0.22 +0.5
2,715,854 + yfiF b2581 0.33 +0.9
2,715,879 - yfiF b2581 0.35 +0.5
2,715,997 + yfiF b2581 0.47 -0.3
2,715,997 + yfiF b2581 0.47 +0.8
2,715,997 + yfiF b2581 0.47 -0.4
2,715,997 + yfiF b2581 0.47 +1.9
2,716,005 - yfiF b2581 0.47 +0.3
2,716,005 - yfiF b2581 0.47 -1.0
2,716,005 - yfiF b2581 0.47 +0.9
2,716,005 - yfiF b2581 0.47 +1.5
2,716,141 + yfiF b2581 0.61 -2.2
2,716,141 + +0.6
2,716,183 - yfiF b2581 0.65 +1.2
2,716,183 - yfiF b2581 0.65 +1.1
2,716,229 + yfiF b2581 0.69 +0.6
2,716,265 - yfiF b2581 0.72 -1.3
2,716,265 - yfiF b2581 0.72 -3.6
2,716,313 + yfiF b2581 0.77 +0.3
2,716,313 + yfiF b2581 0.77 +1.7
2,716,387 - yfiF b2581 0.84 +1.0
2,716,387 - yfiF b2581 0.84 -0.3
2,716,440 + yfiF b2581 0.89 +0.2
2,716,446 + yfiF b2581 0.90 +1.1
2,716,485 + -0.2
2,716,742 + +0.6
2,716,762 - +1.4
2,716,832 + trxC b2582 0.18 +0.7
2,717,016 - trxC b2582 0.62 +0.3
2,717,103 + trxC b2582 0.82 +1.7
2,717,387 - yfiP b2583 0.20 -0.5
2,717,387 - yfiP b2583 0.20 -4.1
2,717,445 + yfiP b2583 0.29 +0.3
2,717,445 + yfiP b2583 0.29 -3.0
2,717,458 - yfiP b2583 0.30 +0.8

Or see this region's nucleotide sequence