Strain Fitness in Escherichia coli BW25113 around b1124

Experiment: Phage_N4_cage_3_mouse_3_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpotD and potC overlap by 4 nucleotidespotC and potB overlap by 4 nucleotidespotB and potA are separated by 13 nucleotides b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052 potD b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843 potC b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667 potB b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817 potA Position (kb) 1182 1183Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 1181.101 kb on + strandat 1181.101 kb on + strandat 1181.170 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.421 kb on + strand, within potDat 1181.480 kb on - strand, within potDat 1181.522 kb on - strand, within potDat 1181.539 kb on + strand, within potDat 1181.547 kb on - strand, within potDat 1181.547 kb on - strand, within potDat 1181.556 kb on + strand, within potDat 1181.556 kb on + strand, within potDat 1181.581 kb on - strand, within potDat 1181.684 kb on - strand, within potDat 1181.721 kb on - strand, within potDat 1181.839 kb on - strand, within potDat 1181.865 kb on - strand, within potDat 1181.918 kb on + strand, within potDat 1182.003 kb on + strandat 1182.395 kb on + strand, within potCat 1182.474 kb on - strand, within potCat 1182.476 kb on - strand, within potCat 1182.524 kb on + strand, within potCat 1182.581 kb on + strand, within potCat 1182.581 kb on + strand, within potCat 1182.589 kb on + strand, within potCat 1182.668 kb on + strand, within potCat 1182.691 kb on + strand, within potCat 1182.700 kb on + strand, within potCat 1182.730 kb on + strand, within potCat 1182.910 kb on + strandat 1182.910 kb on + strandat 1182.914 kb on - strandat 1183.032 kb on + strand, within potBat 1183.032 kb on + strand, within potBat 1183.193 kb on - strand, within potBat 1183.202 kb on + strand, within potBat 1183.202 kb on + strand, within potBat 1183.283 kb on + strand, within potBat 1183.449 kb on + strand, within potBat 1183.542 kb on - strand, within potBat 1183.680 kb on - strandat 1183.698 kb on - strandat 1183.698 kb on - strandat 1183.711 kb on - strandat 1183.711 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_3_Cecum_40 hr
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1,181,101 + -1.1
1,181,101 + +1.9
1,181,170 - potD b1123 0.16 -3.1
1,181,280 - potD b1123 0.26 -3.8
1,181,280 - potD b1123 0.26 -4.4
1,181,421 + potD b1123 0.40 -6.4
1,181,480 - potD b1123 0.45 -3.9
1,181,522 - potD b1123 0.49 -0.3
1,181,539 + potD b1123 0.51 -0.5
1,181,547 - potD b1123 0.52 -0.3
1,181,547 - potD b1123 0.52 -5.9
1,181,556 + potD b1123 0.53 -2.3
1,181,556 + potD b1123 0.53 -2.8
1,181,581 - potD b1123 0.55 -5.0
1,181,684 - potD b1123 0.65 -1.6
1,181,721 - potD b1123 0.68 -2.7
1,181,839 - potD b1123 0.80 -4.4
1,181,865 - potD b1123 0.82 +0.6
1,181,918 + potD b1123 0.87 +0.0
1,182,003 + -0.7
1,182,395 + potC b1124 0.44 -4.5
1,182,474 - potC b1124 0.53 -6.1
1,182,476 - potC b1124 0.54 -1.6
1,182,524 + potC b1124 0.60 -5.3
1,182,581 + potC b1124 0.67 -0.6
1,182,581 + potC b1124 0.67 -2.3
1,182,589 + potC b1124 0.68 +1.2
1,182,668 + potC b1124 0.78 -0.9
1,182,691 + potC b1124 0.81 -2.7
1,182,700 + potC b1124 0.82 -5.4
1,182,730 + potC b1124 0.86 -2.1
1,182,910 + -0.1
1,182,910 + -1.9
1,182,914 - -1.8
1,183,032 + potB b1125 0.23 -3.4
1,183,032 + potB b1125 0.23 -0.9
1,183,193 - potB b1125 0.43 -2.7
1,183,202 + potB b1125 0.44 -2.3
1,183,202 + potB b1125 0.44 +0.5
1,183,283 + potB b1125 0.54 -3.7
1,183,449 + potB b1125 0.74 -2.0
1,183,542 - potB b1125 0.85 -5.5
1,183,680 - +0.8
1,183,698 - -2.2
1,183,698 - -4.0
1,183,711 - -3.9
1,183,711 - -5.6

Or see this region's nucleotide sequence