Strain Fitness in Escherichia coli BW25113 around b4322

Experiment: Phage_N4_cage_3_mouse_2_Cecum_40 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Phage_N4_cage_3_mouse_2_Cecum_40 hr
4,548,745	+	gntP	b4321	0.57	+0.6
4,548,783	+	gntP	b4321	0.60	-0.9
4,548,836	-	gntP	b4321	0.64	+0.7
4,548,836	-	gntP	b4321	0.64	-0.8
4,548,865	+	gntP	b4321	0.66	-0.4
4,548,952	-	gntP	b4321	0.73	+0.6
4,548,981	-	gntP	b4321	0.75	+0.4
4,549,016	+	gntP	b4321	0.77	-1.1
4,549,016	+	gntP	b4321	0.77	+0.5
4,549,024	-	gntP	b4321	0.78	-1.6
4,549,024	-	gntP	b4321	0.78	+0.1
4,549,024	-	gntP	b4321	0.78	+0.5
4,549,024	-	gntP	b4321	0.78	+0.0
4,549,027	-	gntP	b4321	0.78	-1.0
4,549,027	-	gntP	b4321	0.78	-1.2
4,549,029	-	gntP	b4321	0.78	+0.3
4,549,167	+	gntP	b4321	0.89	-0.2
4,549,173	-	gntP	b4321	0.89	+0.1
4,549,175	-	gntP	b4321	0.89	-0.5
4,549,197	-				-2.7
4,549,204	-				+0.6
4,549,265	+				+0.5
4,549,265	+				+0.3
4,549,335	-				-0.8
4,549,335	-				+0.7
4,549,416	-				+0.3
4,549,426	+				-1.0
4,549,456	+				+0.1
4,549,456	+				-0.7
4,549,534	+				-0.0
4,549,534	-				-0.5
4,549,544	+				-0.3
4,549,596	-				-1.4
4,549,596	-				-1.2
4,549,602	-				-0.1
4,549,611	-				+0.1
4,549,618	+				+0.6
4,549,643	-				-3.4
4,549,686	-				-1.3
4,549,759	-				-1.1
4,549,776	-				-0.5
4,549,834	+	uxuA	b4322	0.15	+0.4
4,549,834	+	uxuA	b4322	0.15	-1.0
4,549,835	+	uxuA	b4322	0.15	-3.3
4,549,835	+	uxuA	b4322	0.15	-3.2
4,549,839	+	uxuA	b4322	0.15	-1.4
4,549,839	+	uxuA	b4322	0.15	+0.3
4,549,847	-	uxuA	b4322	0.16	+0.0
4,549,935	+	uxuA	b4322	0.23	-2.0
4,549,990	+	uxuA	b4322	0.28	-2.2
4,550,024	+	uxuA	b4322	0.31	-1.6
4,550,153	-	uxuA	b4322	0.42	-0.8
4,550,185	-	uxuA	b4322	0.44	-0.3
4,550,253	-	uxuA	b4322	0.50	-0.4
4,550,253	-	uxuA	b4322	0.50	-0.4
4,550,255	+	uxuA	b4322	0.50	-0.2
4,550,263	-	uxuA	b4322	0.51	-3.6
4,550,407	+	uxuA	b4322	0.63	-0.8
4,550,409	+	uxuA	b4322	0.63	-1.4
4,550,423	+	uxuA	b4322	0.64	-1.6
4,550,444	-	uxuA	b4322	0.66	-0.9
4,550,444	-	uxuA	b4322	0.66	-0.7
4,550,458	+	uxuA	b4322	0.67	-1.3
4,550,507	+	uxuA	b4322	0.72	-1.2
4,550,563	-	uxuA	b4322	0.76	-2.5
4,550,563	-	uxuA	b4322	0.76	-1.2
4,550,703	+	uxuA	b4322	0.88	-2.7
4,550,712	-	uxuA	b4322	0.89	-0.0
4,550,712	-	uxuA	b4322	0.89	-1.7
4,550,750	-				-1.7
4,550,750	-				-0.9
4,550,829	+				-0.0
4,550,928	+				+0.7
4,550,999	+				-0.7
4,551,012	-				-0.3
4,551,051	-				+0.8
4,551,051	-				+0.9
4,551,051	-				+0.3
4,551,064	-				+0.2
4,551,064	-				-0.2
4,551,154	+	uxuB	b4323	0.16	+1.0
4,551,154	+	uxuB	b4323	0.16	-0.1
4,551,160	-	uxuB	b4323	0.16	+0.4
4,551,196	+	uxuB	b4323	0.19	-0.3
4,551,196	+	uxuB	b4323	0.19	-0.2
4,551,254	+	uxuB	b4323	0.23	+0.5
4,551,589	+	uxuB	b4323	0.46	-1.5
4,551,657	+	uxuB	b4323	0.50	+0.6
4,551,657	+	uxuB	b4323	0.50	-0.2
4,551,678	-	uxuB	b4323	0.52	-1.0
4,551,680	-	uxuB	b4323	0.52	-0.3
4,551,708	-	uxuB	b4323	0.54	-0.6
4,551,749	-	uxuB	b4323	0.56	-1.9
4,551,760	+	uxuB	b4323	0.57	-0.7
4,551,760	+	uxuB	b4323	0.57	+0.1
4,551,765	+	uxuB	b4323	0.58	+0.5
4,551,771	+	uxuB	b4323	0.58	+0.4
4,551,824	+	uxuB	b4323	0.62	+0.1