Experiment: Phage_N4_cage_3_mouse_2_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gshB and yqgE are separated by 108 nucleotides yqgE and yqgF overlap by 1 nucleotides yqgF and yggR are separated by 183 nucleotides
b2947: gshB - glutathione synthetase (NCBI), at 3,089,900 to 3,090,850
gshB
b2948: yqgE - orf, hypothetical protein (VIMSS), at 3,090,959 to 3,091,522
yqgE
b2949: yqgF - Holliday junction resolvase-like protein (NCBI), at 3,091,522 to 3,091,938
yqgF
b2950: yggR - putative protein transport (VIMSS), at 3,092,122 to 3,093,102
yggR
Position (kb)
3090
3091
3092 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3090.004 kb on + strand, within gshB at 3090.085 kb on - strand, within gshB at 3090.085 kb on - strand, within gshB at 3090.092 kb on - strand, within gshB at 3090.222 kb on + strand, within gshB at 3090.458 kb on - strand, within gshB at 3090.479 kb on - strand, within gshB at 3090.479 kb on - strand, within gshB at 3090.486 kb on - strand, within gshB at 3090.562 kb on - strand, within gshB at 3090.562 kb on - strand, within gshB at 3090.576 kb on + strand, within gshB at 3090.811 kb on + strand at 3090.847 kb on - strand at 3090.847 kb on - strand at 3090.883 kb on + strand at 3090.883 kb on + strand at 3090.893 kb on - strand at 3090.893 kb on - strand at 3091.078 kb on - strand, within yqgE at 3091.078 kb on - strand, within yqgE at 3091.140 kb on + strand, within yqgE at 3091.140 kb on + strand, within yqgE at 3091.271 kb on - strand, within yqgE at 3091.271 kb on - strand, within yqgE at 3091.328 kb on + strand, within yqgE at 3091.534 kb on + strand at 3091.929 kb on + strand at 3091.942 kb on + strand at 3091.973 kb on - strand at 3091.973 kb on - strand at 3091.982 kb on - strand at 3092.008 kb on - strand at 3092.032 kb on + strand at 3092.032 kb on + strand at 3092.034 kb on + strand at 3092.053 kb on + strand at 3092.156 kb on - strand at 3092.156 kb on - strand at 3092.336 kb on - strand, within yggR at 3092.338 kb on + strand, within yggR at 3092.338 kb on + strand, within yggR at 3092.357 kb on - strand, within yggR at 3092.395 kb on + strand, within yggR at 3092.398 kb on + strand, within yggR
Per-strain Table
Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_2_Cecum_40 hr remove 3,090,004 + gshB b2947 0.11 -2.0 3,090,085 - gshB b2947 0.19 -1.1 3,090,085 - gshB b2947 0.19 -1.6 3,090,092 - gshB b2947 0.20 -0.8 3,090,222 + gshB b2947 0.34 +1.2 3,090,458 - gshB b2947 0.59 -3.8 3,090,479 - gshB b2947 0.61 -0.3 3,090,479 - gshB b2947 0.61 -1.6 3,090,486 - gshB b2947 0.62 -0.6 3,090,562 - gshB b2947 0.70 -0.2 3,090,562 - gshB b2947 0.70 -1.6 3,090,576 + gshB b2947 0.71 -3.2 3,090,811 + -0.8 3,090,847 - -1.3 3,090,847 - +0.8 3,090,883 + +0.9 3,090,883 + +0.1 3,090,893 - -0.2 3,090,893 - +0.2 3,091,078 - yqgE b2948 0.21 -3.2 3,091,078 - yqgE b2948 0.21 -2.5 3,091,140 + yqgE b2948 0.32 -1.6 3,091,140 + yqgE b2948 0.32 -0.1 3,091,271 - yqgE b2948 0.55 -2.9 3,091,271 - yqgE b2948 0.55 -1.5 3,091,328 + yqgE b2948 0.65 +0.4 3,091,534 + +0.2 3,091,929 + +0.3 3,091,942 + +0.4 3,091,973 - -0.3 3,091,973 - +0.9 3,091,982 - +0.4 3,092,008 - +1.2 3,092,032 + +0.2 3,092,032 + -0.2 3,092,034 + +0.1 3,092,053 + -0.5 3,092,156 - -0.3 3,092,156 - +0.0 3,092,336 - yggR b2950 0.22 -0.1 3,092,338 + yggR b2950 0.22 +0.4 3,092,338 + yggR b2950 0.22 -0.4 3,092,357 - yggR b2950 0.24 +0.4 3,092,395 + yggR b2950 0.28 +0.5 3,092,398 + yggR b2950 0.28 +0.6
Or see this region's nucleotide sequence