Experiment: Phage_N4_cage_3_mouse_2_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcl and hyi are separated by 12 nucleotides hyi and glxR are separated by 99 nucleotides glxR and ybbW are separated by 168 nucleotides
b0507: gcl - glyoxylate carboligase (NCBI), at 533,140 to 534,921
gcl
b0508: hyi - hydroxypyruvate isomerase (NCBI), at 534,934 to 535,710
hyi
b0509: glxR - tartronate semialdehyde reductase, NADH-dependent (NCBI), at 535,810 to 536,688
glxR
b0511: ybbW - putative transport protein (VIMSS), at 536,857 to 538,311
ybbW
Position (kb)
535
536
537 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 534.830 kb on + strand at 534.830 kb on + strand at 534.830 kb on + strand at 534.833 kb on + strand at 534.833 kb on + strand at 534.835 kb on + strand at 534.835 kb on + strand at 534.843 kb on - strand at 534.865 kb on + strand at 534.868 kb on + strand at 534.868 kb on + strand at 534.868 kb on + strand at 534.878 kb on + strand at 534.878 kb on + strand at 534.889 kb on + strand at 534.891 kb on + strand at 534.891 kb on + strand at 534.892 kb on + strand at 534.892 kb on + strand at 534.899 kb on - strand at 534.911 kb on + strand at 534.912 kb on + strand at 534.912 kb on + strand at 535.001 kb on + strand at 535.011 kb on + strand at 535.128 kb on - strand, within hyi at 535.143 kb on - strand, within hyi at 535.159 kb on + strand, within hyi at 535.162 kb on - strand, within hyi at 535.162 kb on - strand, within hyi at 535.207 kb on + strand, within hyi at 535.207 kb on + strand, within hyi at 535.207 kb on + strand, within hyi at 535.214 kb on + strand, within hyi at 535.214 kb on + strand, within hyi at 535.267 kb on + strand, within hyi at 535.356 kb on + strand, within hyi at 535.403 kb on + strand, within hyi at 535.435 kb on + strand, within hyi at 535.485 kb on + strand, within hyi at 535.511 kb on + strand, within hyi at 535.532 kb on - strand, within hyi at 535.681 kb on + strand at 535.686 kb on + strand at 535.691 kb on + strand at 535.691 kb on + strand at 535.696 kb on + strand at 535.704 kb on - strand at 535.717 kb on + strand at 535.717 kb on + strand at 535.722 kb on + strand at 535.759 kb on - strand at 535.759 kb on - strand at 535.760 kb on + strand at 535.760 kb on + strand at 535.760 kb on + strand at 535.841 kb on + strand at 535.849 kb on - strand at 535.860 kb on - strand at 535.882 kb on + strand at 535.890 kb on - strand at 535.892 kb on - strand at 535.952 kb on - strand, within glxR at 535.967 kb on - strand, within glxR at 535.969 kb on - strand, within glxR at 535.998 kb on + strand, within glxR at 536.006 kb on + strand, within glxR at 536.007 kb on + strand, within glxR at 536.007 kb on + strand, within glxR at 536.048 kb on - strand, within glxR at 536.128 kb on + strand, within glxR at 536.174 kb on + strand, within glxR at 536.187 kb on + strand, within glxR at 536.195 kb on - strand, within glxR at 536.295 kb on - strand, within glxR at 536.308 kb on - strand, within glxR at 536.393 kb on - strand, within glxR at 536.429 kb on + strand, within glxR at 536.492 kb on - strand, within glxR at 536.522 kb on + strand, within glxR at 536.533 kb on + strand, within glxR at 536.533 kb on + strand, within glxR at 536.536 kb on - strand, within glxR at 536.595 kb on + strand, within glxR at 536.595 kb on + strand, within glxR at 536.626 kb on + strand at 536.626 kb on + strand at 536.631 kb on + strand at 536.636 kb on + strand at 536.637 kb on - strand at 536.639 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.658 kb on + strand at 536.658 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.673 kb on + strand at 536.982 kb on - strand at 536.984 kb on - strand at 537.041 kb on - strand, within ybbW at 537.096 kb on - strand, within ybbW at 537.096 kb on - strand, within ybbW at 537.106 kb on - strand, within ybbW at 537.116 kb on - strand, within ybbW at 537.119 kb on - strand, within ybbW at 537.119 kb on - strand, within ybbW at 537.119 kb on - strand, within ybbW at 537.121 kb on - strand, within ybbW at 537.146 kb on + strand, within ybbW at 537.152 kb on + strand, within ybbW at 537.155 kb on + strand, within ybbW at 537.155 kb on + strand, within ybbW at 537.155 kb on + strand, within ybbW at 537.155 kb on - strand, within ybbW at 537.157 kb on + strand, within ybbW at 537.163 kb on - strand, within ybbW at 537.171 kb on + strand, within ybbW at 537.207 kb on + strand, within ybbW at 537.215 kb on - strand, within ybbW at 537.215 kb on - strand, within ybbW at 537.225 kb on + strand, within ybbW at 537.225 kb on + strand, within ybbW at 537.227 kb on + strand, within ybbW at 537.227 kb on + strand, within ybbW at 537.235 kb on - strand, within ybbW at 537.235 kb on - strand, within ybbW at 537.237 kb on + strand, within ybbW at 537.237 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.239 kb on - strand, within ybbW at 537.246 kb on - strand, within ybbW at 537.246 kb on - strand, within ybbW at 537.248 kb on + strand, within ybbW at 537.248 kb on + strand, within ybbW at 537.256 kb on - strand, within ybbW at 537.256 kb on - strand, within ybbW at 537.299 kb on + strand, within ybbW at 537.307 kb on + strand, within ybbW at 537.307 kb on + strand, within ybbW at 537.309 kb on + strand, within ybbW at 537.315 kb on - strand, within ybbW at 537.327 kb on - strand, within ybbW at 537.362 kb on + strand, within ybbW at 537.362 kb on + strand, within ybbW at 537.367 kb on + strand, within ybbW at 537.368 kb on + strand, within ybbW at 537.368 kb on + strand, within ybbW at 537.421 kb on - strand, within ybbW at 537.423 kb on - strand, within ybbW at 537.423 kb on - strand, within ybbW at 537.437 kb on - strand, within ybbW at 537.437 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.517 kb on - strand, within ybbW at 537.551 kb on + strand, within ybbW at 537.551 kb on + strand, within ybbW at 537.552 kb on + strand, within ybbW at 537.565 kb on + strand, within ybbW at 537.583 kb on - strand, within ybbW at 537.583 kb on - strand, within ybbW at 537.644 kb on + strand, within ybbW at 537.644 kb on + strand, within ybbW at 537.659 kb on + strand, within ybbW at 537.669 kb on - strand, within ybbW at 537.669 kb on - strand, within ybbW at 537.676 kb on + strand, within ybbW at 537.678 kb on + strand, within ybbW
Per-strain Table
Position Strand Gene LocusTag Fraction Phage_N4_cage_3_mouse_2_Cecum_40 hr remove 534,830 + -1.0 534,830 + -1.0 534,830 + -3.4 534,833 + +0.6 534,833 + -0.7 534,835 + +0.3 534,835 + +0.4 534,843 - +1.9 534,865 + -0.2 534,868 + -1.0 534,868 + +0.1 534,868 + -0.5 534,878 + +1.0 534,878 + +1.3 534,889 + -2.1 534,891 + +1.3 534,891 + +1.5 534,892 + -4.4 534,892 + -0.1 534,899 - +1.9 534,911 + +1.1 534,912 + +0.7 534,912 + +0.3 535,001 + +1.2 535,011 + -0.7 535,128 - hyi b0508 0.25 +0.9 535,143 - hyi b0508 0.27 +1.9 535,159 + hyi b0508 0.29 -0.4 535,162 - hyi b0508 0.29 +1.9 535,162 - hyi b0508 0.29 +1.9 535,207 + hyi b0508 0.35 -3.1 535,207 + hyi b0508 0.35 -0.2 535,207 + hyi b0508 0.35 +0.4 535,214 + hyi b0508 0.36 +0.7 535,214 + hyi b0508 0.36 -0.1 535,267 + hyi b0508 0.43 +0.2 535,356 + hyi b0508 0.54 -0.8 535,403 + hyi b0508 0.60 -0.6 535,435 + hyi b0508 0.64 +1.0 535,485 + hyi b0508 0.71 -0.1 535,511 + hyi b0508 0.74 -2.9 535,532 - hyi b0508 0.77 +1.9 535,681 + -0.5 535,686 + +0.9 535,691 + -1.3 535,691 + -0.8 535,696 + -0.6 535,704 - +0.6 535,717 + -0.4 535,717 + +0.2 535,722 + +0.4 535,759 - +2.9 535,759 - +0.5 535,760 + +1.3 535,760 + +0.3 535,760 + +0.3 535,841 + -1.4 535,849 - -1.5 535,860 - -2.8 535,882 + -2.6 535,890 - +0.4 535,892 - -3.6 535,952 - glxR b0509 0.16 +0.2 535,967 - glxR b0509 0.18 -1.3 535,969 - glxR b0509 0.18 -2.5 535,998 + glxR b0509 0.21 -1.8 536,006 + glxR b0509 0.22 -0.2 536,007 + glxR b0509 0.22 +0.4 536,007 + glxR b0509 0.22 +0.3 536,048 - glxR b0509 0.27 -0.1 536,128 + glxR b0509 0.36 +0.1 536,174 + glxR b0509 0.41 -0.4 536,187 + glxR b0509 0.43 +0.6 536,195 - glxR b0509 0.44 +1.1 536,295 - glxR b0509 0.55 -0.3 536,308 - glxR b0509 0.57 -3.5 536,393 - glxR b0509 0.66 +1.1 536,429 + glxR b0509 0.70 -0.0 536,492 - glxR b0509 0.78 -2.0 536,522 + glxR b0509 0.81 -0.2 536,533 + glxR b0509 0.82 +0.0 536,533 + glxR b0509 0.82 -3.2 536,536 - glxR b0509 0.83 -0.4 536,595 + glxR b0509 0.89 -0.3 536,595 + glxR b0509 0.89 -0.6 536,626 + +0.4 536,626 + +0.9 536,631 + -0.2 536,636 + -0.3 536,637 - +0.9 536,639 + -1.0 536,641 + -0.2 536,641 + -4.4 536,641 + -0.3 536,641 + -0.0 536,658 + +1.2 536,658 + -0.4 536,672 + +0.9 536,672 + +0.7 536,672 + -0.4 536,672 + -0.7 536,672 + +1.0 536,672 + -0.2 536,672 + +0.2 536,672 + -0.5 536,672 + +0.3 536,672 + +1.1 536,672 + +0.9 536,672 + +0.5 536,672 + -0.4 536,672 + -0.2 536,673 + +0.1 536,982 - -0.2 536,984 - +0.4 537,041 - ybbW b0511 0.13 -2.4 537,096 - ybbW b0511 0.16 -0.6 537,096 - ybbW b0511 0.16 +1.6 537,106 - ybbW b0511 0.17 +0.8 537,116 - ybbW b0511 0.18 +0.2 537,119 - ybbW b0511 0.18 +2.4 537,119 - ybbW b0511 0.18 -0.8 537,119 - ybbW b0511 0.18 +1.0 537,121 - ybbW b0511 0.18 +1.6 537,146 + ybbW b0511 0.20 +0.9 537,152 + ybbW b0511 0.20 +1.9 537,155 + ybbW b0511 0.20 -1.8 537,155 + ybbW b0511 0.20 +0.9 537,155 + ybbW b0511 0.20 -1.1 537,155 - ybbW b0511 0.20 +1.4 537,157 + ybbW b0511 0.21 +0.4 537,163 - ybbW b0511 0.21 +0.3 537,171 + ybbW b0511 0.22 +2.5 537,207 + ybbW b0511 0.24 +0.4 537,215 - ybbW b0511 0.25 -0.1 537,215 - ybbW b0511 0.25 -2.0 537,225 + ybbW b0511 0.25 -4.2 537,225 + ybbW b0511 0.25 -1.3 537,227 + ybbW b0511 0.25 -0.5 537,227 + ybbW b0511 0.25 -1.1 537,235 - ybbW b0511 0.26 +2.5 537,235 - ybbW b0511 0.26 -1.9 537,237 + ybbW b0511 0.26 +0.6 537,237 + ybbW b0511 0.26 -2.1 537,238 + ybbW b0511 0.26 +2.5 537,238 + ybbW b0511 0.26 -2.6 537,238 + ybbW b0511 0.26 -2.4 537,238 + ybbW b0511 0.26 +1.1 537,238 + ybbW b0511 0.26 -0.8 537,239 - ybbW b0511 0.26 +4.1 537,246 - ybbW b0511 0.27 -0.6 537,246 - ybbW b0511 0.27 +5.3 537,248 + ybbW b0511 0.27 -1.9 537,248 + ybbW b0511 0.27 -3.5 537,256 - ybbW b0511 0.27 +2.2 537,256 - ybbW b0511 0.27 -2.1 537,299 + ybbW b0511 0.30 -0.2 537,307 + ybbW b0511 0.31 +0.7 537,307 + ybbW b0511 0.31 -0.9 537,309 + ybbW b0511 0.31 +0.2 537,315 - ybbW b0511 0.31 +1.9 537,327 - ybbW b0511 0.32 +1.9 537,362 + ybbW b0511 0.35 +0.2 537,362 + ybbW b0511 0.35 -0.4 537,367 + ybbW b0511 0.35 -0.6 537,368 + ybbW b0511 0.35 -0.4 537,368 + ybbW b0511 0.35 +0.3 537,421 - ybbW b0511 0.39 +0.3 537,423 - ybbW b0511 0.39 +0.2 537,423 - ybbW b0511 0.39 +2.1 537,437 - ybbW b0511 0.40 +1.4 537,437 - ybbW b0511 0.40 +3.5 537,479 - ybbW b0511 0.43 -1.4 537,479 - ybbW b0511 0.43 -0.6 537,479 - ybbW b0511 0.43 +0.5 537,479 - ybbW b0511 0.43 +1.9 537,479 - ybbW b0511 0.43 -3.0 537,517 - ybbW b0511 0.45 -0.1 537,551 + ybbW b0511 0.48 -1.5 537,551 + ybbW b0511 0.48 -1.2 537,552 + ybbW b0511 0.48 -0.4 537,565 + ybbW b0511 0.49 +0.1 537,583 - ybbW b0511 0.50 -0.9 537,583 - ybbW b0511 0.50 -0.1 537,644 + ybbW b0511 0.54 -0.3 537,644 + ybbW b0511 0.54 -0.3 537,659 + ybbW b0511 0.55 -0.4 537,669 - ybbW b0511 0.56 -0.9 537,669 - ybbW b0511 0.56 -0.4 537,676 + ybbW b0511 0.56 +1.3 537,678 + ybbW b0511 0.56 -1.0
Or see this region's nucleotide sequence