Experiment: PBS_cage_2_mouse_3_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgcR and sgcE are separated by 16 nucleotides sgcE and sgcA are separated by 11 nucleotides sgcA and ryjB overlap by 4 nucleotides ryjB and sgcQ are separated by 44 nucleotides sgcQ and sgcC are separated by 12 nucleotides
b4300: sgcR - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,524,129 to 4,524,911
sgcR
b4301: sgcE - ribulose-phosphate 3-epimerase (NCBI), at 4,524,928 to 4,525,560
sgcE
b4302: sgcA - KpLE2 phage-like element; predicted phosphotransferase enzyme IIA component (NCBI), at 4,525,572 to 4,526,003
sgcA
b4624: ryjB - Novel sRNA, function unknown (RefSeq), at 4,526,000 to 4,526,089
ryjB
b4303: sgcQ - KpLE2 phage-like element; predicted nucleoside triphosphatase (NCBI), at 4,526,134 to 4,526,940
sgcQ
b4304: sgcC - KpLE2 phage-like element; predicted phosphotransferase enzyme IIC component (NCBI), at 4,526,953 to 4,528,266
sgcC
Position (kb)
4525
4526
4527 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 4524.579 kb on - strand, within sgcR at 4524.723 kb on - strand, within sgcR at 4524.723 kb on - strand, within sgcR at 4524.726 kb on - strand, within sgcR at 4524.771 kb on - strand, within sgcR at 4524.831 kb on + strand, within sgcR at 4524.847 kb on - strand at 4524.881 kb on + strand at 4524.898 kb on - strand at 4524.924 kb on + strand at 4524.968 kb on + strand at 4524.968 kb on + strand at 4524.976 kb on + strand at 4525.061 kb on - strand, within sgcE at 4525.078 kb on - strand, within sgcE at 4525.167 kb on - strand, within sgcE at 4525.217 kb on - strand, within sgcE at 4525.220 kb on + strand, within sgcE at 4525.220 kb on + strand, within sgcE at 4525.290 kb on - strand, within sgcE at 4525.315 kb on - strand, within sgcE at 4525.315 kb on - strand, within sgcE at 4525.363 kb on + strand, within sgcE at 4525.462 kb on + strand, within sgcE at 4525.492 kb on + strand, within sgcE at 4525.492 kb on + strand, within sgcE at 4525.519 kb on + strand at 4525.549 kb on + strand at 4525.559 kb on + strand at 4525.565 kb on - strand at 4525.632 kb on - strand, within sgcA at 4525.632 kb on - strand, within sgcA at 4525.650 kb on + strand, within sgcA at 4525.663 kb on - strand, within sgcA at 4525.756 kb on + strand, within sgcA at 4525.761 kb on + strand, within sgcA at 4525.805 kb on - strand, within sgcA at 4525.805 kb on - strand, within sgcA at 4525.844 kb on + strand, within sgcA at 4525.888 kb on + strand, within sgcA at 4525.888 kb on + strand, within sgcA at 4525.896 kb on + strand, within sgcA at 4525.896 kb on + strand, within sgcA at 4525.914 kb on + strand, within sgcA at 4525.971 kb on - strand at 4526.051 kb on + strand, within ryjB at 4526.140 kb on + strand at 4526.140 kb on + strand at 4526.140 kb on + strand at 4526.164 kb on + strand at 4526.174 kb on + strand at 4526.174 kb on + strand at 4526.254 kb on + strand, within sgcQ at 4526.254 kb on + strand, within sgcQ at 4526.257 kb on + strand, within sgcQ at 4526.298 kb on + strand, within sgcQ at 4526.338 kb on - strand at 4526.356 kb on + strand, within sgcQ at 4526.356 kb on + strand, within sgcQ at 4526.388 kb on + strand, within sgcQ at 4526.448 kb on + strand, within sgcQ at 4526.448 kb on + strand, within sgcQ at 4526.460 kb on + strand, within sgcQ at 4526.461 kb on + strand, within sgcQ at 4526.468 kb on - strand, within sgcQ at 4526.468 kb on - strand, within sgcQ at 4526.583 kb on - strand, within sgcQ at 4526.621 kb on - strand, within sgcQ at 4526.738 kb on + strand, within sgcQ at 4526.769 kb on - strand, within sgcQ at 4526.774 kb on + strand, within sgcQ at 4526.809 kb on + strand, within sgcQ at 4526.874 kb on + strand at 4526.885 kb on - strand at 4526.885 kb on - strand at 4526.895 kb on - strand at 4526.975 kb on + strand at 4526.975 kb on + strand at 4526.993 kb on - strand at 4526.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PBS_cage_2_mouse_3_Cecum_40 hr remove 4,524,579 - sgcR b4300 0.57 +2.6 4,524,723 - sgcR b4300 0.76 +1.5 4,524,723 - sgcR b4300 0.76 -1.5 4,524,726 - sgcR b4300 0.76 -3.2 4,524,771 - sgcR b4300 0.82 +1.0 4,524,831 + sgcR b4300 0.90 -0.3 4,524,847 - -4.4 4,524,881 + +0.2 4,524,898 - +0.7 4,524,924 + +0.3 4,524,968 + -0.5 4,524,968 + -5.0 4,524,976 + +0.2 4,525,061 - sgcE b4301 0.21 +1.0 4,525,078 - sgcE b4301 0.24 +1.3 4,525,167 - sgcE b4301 0.38 -0.9 4,525,217 - sgcE b4301 0.46 +2.0 4,525,220 + sgcE b4301 0.46 -0.1 4,525,220 + sgcE b4301 0.46 +0.4 4,525,290 - sgcE b4301 0.57 +0.2 4,525,315 - sgcE b4301 0.61 +0.5 4,525,315 - sgcE b4301 0.61 +0.5 4,525,363 + sgcE b4301 0.69 +0.5 4,525,462 + sgcE b4301 0.84 +0.6 4,525,492 + sgcE b4301 0.89 +0.0 4,525,492 + sgcE b4301 0.89 +2.7 4,525,519 + -0.3 4,525,549 + +0.4 4,525,559 + -0.1 4,525,565 - +0.9 4,525,632 - sgcA b4302 0.14 +1.6 4,525,632 - sgcA b4302 0.14 +0.5 4,525,650 + sgcA b4302 0.18 +0.5 4,525,663 - sgcA b4302 0.21 -2.1 4,525,756 + sgcA b4302 0.43 -0.4 4,525,761 + sgcA b4302 0.44 -0.7 4,525,805 - sgcA b4302 0.54 -1.0 4,525,805 - sgcA b4302 0.54 -0.1 4,525,844 + sgcA b4302 0.63 -0.9 4,525,888 + sgcA b4302 0.73 +0.6 4,525,888 + sgcA b4302 0.73 +0.0 4,525,896 + sgcA b4302 0.75 -0.2 4,525,896 + sgcA b4302 0.75 -1.8 4,525,914 + sgcA b4302 0.79 +0.1 4,525,971 - +0.9 4,526,051 + ryjB b4624 0.57 -0.2 4,526,140 + +5.7 4,526,140 + +0.3 4,526,140 + +5.5 4,526,164 + -1.1 4,526,174 + -0.7 4,526,174 + -0.4 4,526,254 + sgcQ b4303 0.15 +0.5 4,526,254 + sgcQ b4303 0.15 -1.7 4,526,257 + sgcQ b4303 0.15 -0.8 4,526,298 + sgcQ b4303 0.20 +0.4 4,526,338 - +1.5 4,526,356 + sgcQ b4303 0.28 +0.8 4,526,356 + sgcQ b4303 0.28 +0.7 4,526,388 + sgcQ b4303 0.31 +0.0 4,526,448 + sgcQ b4303 0.39 -1.3 4,526,448 + sgcQ b4303 0.39 -0.8 4,526,460 + sgcQ b4303 0.40 +1.1 4,526,461 + sgcQ b4303 0.41 +0.4 4,526,468 - sgcQ b4303 0.41 -0.2 4,526,468 - sgcQ b4303 0.41 +3.7 4,526,583 - sgcQ b4303 0.56 +2.8 4,526,621 - sgcQ b4303 0.60 +0.3 4,526,738 + sgcQ b4303 0.75 +2.2 4,526,769 - sgcQ b4303 0.79 +0.6 4,526,774 + sgcQ b4303 0.79 -1.1 4,526,809 + sgcQ b4303 0.84 +0.6 4,526,874 + -0.6 4,526,885 - -1.4 4,526,885 - -1.1 4,526,895 - +0.3 4,526,975 + -0.6 4,526,975 + -1.1 4,526,993 - +0.3 4,526,993 - -0.3
Or see this region's nucleotide sequence