Experiment: PBS_cage_2_mouse_1_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspF and gspG are separated by 9 nucleotides gspG and gspH are separated by 7 nucleotides gspH and yheH overlap by 4 nucleotides yheH and gspJ overlap by 8 nucleotides gspJ and gspK overlap by 8 nucleotides
b3327: gspF - general secretory pathway component, cryptic (NCBI), at 3,457,839 to 3,459,035
gspF
b3328: gspG - pseudopilin, cryptic, general secretion pathway (NCBI), at 3,459,045 to 3,459,482
gspG
b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999
gspH
b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373
yheH
b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953
gspJ
b3332: gspK - general secretory pathway component, cryptic (NCBI), at 3,460,946 to 3,461,929
gspK
Position (kb)
3459
3460
3461 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 3459.001 kb on + strand at 3459.004 kb on - strand at 3459.015 kb on - strand at 3459.027 kb on - strand at 3459.078 kb on - strand at 3459.078 kb on - strand at 3459.203 kb on - strand, within gspG at 3459.203 kb on - strand, within gspG at 3459.226 kb on - strand, within gspG at 3459.244 kb on - strand, within gspG at 3459.252 kb on + strand, within gspG at 3459.252 kb on + strand, within gspG at 3459.288 kb on - strand, within gspG at 3459.288 kb on - strand, within gspG at 3459.320 kb on + strand, within gspG at 3459.320 kb on + strand, within gspG at 3459.324 kb on + strand, within gspG at 3459.355 kb on - strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.368 kb on - strand, within gspG at 3459.371 kb on + strand, within gspG at 3459.389 kb on - strand, within gspG at 3459.391 kb on + strand, within gspG at 3459.391 kb on + strand, within gspG at 3459.391 kb on + strand, within gspG at 3459.399 kb on - strand, within gspG at 3459.421 kb on + strand, within gspG at 3459.446 kb on - strand at 3459.460 kb on - strand at 3459.460 kb on - strand at 3459.519 kb on - strand at 3459.519 kb on - strand at 3459.530 kb on - strand at 3459.530 kb on - strand at 3459.540 kb on - strand at 3459.570 kb on + strand, within gspH at 3459.578 kb on - strand, within gspH at 3459.603 kb on - strand, within gspH at 3459.607 kb on - strand, within gspH at 3459.607 kb on - strand, within gspH at 3459.674 kb on - strand, within gspH at 3459.698 kb on - strand, within gspH at 3459.716 kb on + strand, within gspH at 3459.742 kb on + strand, within gspH at 3459.742 kb on + strand, within gspH at 3459.747 kb on + strand, within gspH at 3459.818 kb on + strand, within gspH at 3459.851 kb on - strand, within gspH at 3459.851 kb on - strand, within gspH at 3459.893 kb on + strand, within gspH at 3459.920 kb on - strand, within gspH at 3459.930 kb on + strand, within gspH at 3459.937 kb on + strand, within gspH at 3459.937 kb on + strand, within gspH at 3459.947 kb on + strand, within gspH at 3459.947 kb on + strand, within gspH at 3459.955 kb on - strand at 3459.964 kb on - strand at 3459.978 kb on + strand at 3459.978 kb on + strand at 3459.978 kb on + strand at 3459.995 kb on + strand at 3460.012 kb on + strand at 3460.012 kb on + strand at 3460.031 kb on - strand at 3460.043 kb on - strand, within yheH at 3460.057 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.083 kb on + strand, within yheH at 3460.083 kb on + strand, within yheH at 3460.086 kb on - strand, within yheH at 3460.086 kb on - strand, within yheH at 3460.086 kb on - strand, within yheH at 3460.089 kb on - strand, within yheH at 3460.089 kb on - strand, within yheH at 3460.107 kb on + strand, within yheH at 3460.114 kb on + strand, within yheH at 3460.133 kb on + strand, within yheH at 3460.198 kb on - strand, within yheH at 3460.198 kb on - strand, within yheH at 3460.224 kb on + strand, within yheH at 3460.248 kb on - strand, within yheH at 3460.248 kb on - strand, within yheH at 3460.248 kb on - strand, within yheH at 3460.277 kb on - strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.385 kb on + strand at 3460.388 kb on - strand at 3460.395 kb on + strand at 3460.395 kb on + strand at 3460.401 kb on + strand at 3460.439 kb on - strand, within gspJ at 3460.518 kb on + strand at 3460.524 kb on + strand, within gspJ at 3460.529 kb on - strand, within gspJ at 3460.534 kb on - strand, within gspJ at 3460.538 kb on - strand, within gspJ at 3460.569 kb on - strand, within gspJ at 3460.569 kb on - strand, within gspJ at 3460.580 kb on - strand, within gspJ at 3460.592 kb on - strand, within gspJ at 3460.596 kb on + strand, within gspJ at 3460.639 kb on - strand, within gspJ at 3460.666 kb on + strand at 3460.667 kb on + strand, within gspJ at 3460.668 kb on - strand, within gspJ at 3460.674 kb on - strand, within gspJ at 3460.686 kb on - strand, within gspJ at 3460.686 kb on - strand, within gspJ at 3460.705 kb on - strand, within gspJ at 3460.705 kb on - strand, within gspJ at 3460.707 kb on + strand, within gspJ at 3460.713 kb on - strand, within gspJ at 3460.713 kb on - strand, within gspJ at 3460.725 kb on - strand, within gspJ at 3460.725 kb on - strand, within gspJ at 3460.727 kb on - strand, within gspJ at 3460.732 kb on - strand, within gspJ at 3460.734 kb on - strand, within gspJ at 3460.770 kb on - strand, within gspJ at 3460.770 kb on - strand, within gspJ at 3460.770 kb on - strand, within gspJ at 3460.771 kb on - strand, within gspJ at 3460.835 kb on + strand, within gspJ at 3460.835 kb on + strand, within gspJ at 3460.848 kb on + strand, within gspJ at 3460.848 kb on + strand, within gspJ at 3460.854 kb on + strand, within gspJ at 3460.870 kb on - strand, within gspJ at 3460.872 kb on - strand, within gspJ at 3460.872 kb on - strand, within gspJ at 3460.877 kb on + strand, within gspJ at 3460.890 kb on - strand, within gspJ at 3460.900 kb on + strand at 3460.900 kb on + strand at 3460.937 kb on - strand at 3460.937 kb on - strand at 3460.978 kb on - strand at 3460.978 kb on - strand at 3460.982 kb on + strand at 3460.982 kb on + strand at 3461.004 kb on + strand at 3461.021 kb on + strand at 3461.045 kb on - strand, within gspK at 3461.045 kb on - strand, within gspK at 3461.094 kb on + strand, within gspK at 3461.129 kb on - strand, within gspK at 3461.132 kb on + strand, within gspK at 3461.139 kb on - strand, within gspK at 3461.147 kb on + strand, within gspK at 3461.176 kb on + strand, within gspK at 3461.176 kb on + strand, within gspK at 3461.176 kb on + strand, within gspK at 3461.176 kb on + strand, within gspK at 3461.178 kb on + strand, within gspK at 3461.178 kb on + strand, within gspK at 3461.184 kb on - strand, within gspK at 3461.186 kb on - strand, within gspK at 3461.186 kb on - strand, within gspK at 3461.186 kb on - strand, within gspK at 3461.201 kb on + strand, within gspK at 3461.249 kb on - strand, within gspK at 3461.253 kb on + strand, within gspK at 3461.261 kb on - strand, within gspK at 3461.289 kb on + strand, within gspK at 3461.320 kb on + strand, within gspK at 3461.323 kb on + strand, within gspK at 3461.343 kb on - strand, within gspK at 3461.343 kb on - strand, within gspK at 3461.348 kb on + strand, within gspK at 3461.348 kb on + strand, within gspK at 3461.348 kb on + strand, within gspK at 3461.352 kb on - strand, within gspK
Per-strain Table
Position Strand Gene LocusTag Fraction PBS_cage_2_mouse_1_Cecum_40 hr remove 3,459,001 + -0.8 3,459,004 - -3.6 3,459,015 - -0.5 3,459,027 - -0.5 3,459,078 - -2.4 3,459,078 - +0.4 3,459,203 - gspG b3328 0.36 -2.4 3,459,203 - gspG b3328 0.36 +0.0 3,459,226 - gspG b3328 0.41 +0.2 3,459,244 - gspG b3328 0.45 +0.4 3,459,252 + gspG b3328 0.47 -5.5 3,459,252 + gspG b3328 0.47 +1.0 3,459,288 - gspG b3328 0.55 -1.5 3,459,288 - gspG b3328 0.55 -0.2 3,459,320 + gspG b3328 0.63 +0.2 3,459,320 + gspG b3328 0.63 -0.6 3,459,324 + gspG b3328 0.64 -0.3 3,459,355 - gspG b3328 0.71 -4.2 3,459,360 + gspG b3328 0.72 +1.7 3,459,360 + gspG b3328 0.72 -5.1 3,459,360 + gspG b3328 0.72 -0.8 3,459,360 + gspG b3328 0.72 -1.5 3,459,368 - gspG b3328 0.74 -0.6 3,459,371 + gspG b3328 0.74 +1.0 3,459,389 - gspG b3328 0.79 -0.2 3,459,391 + gspG b3328 0.79 -0.3 3,459,391 + gspG b3328 0.79 +2.0 3,459,391 + gspG b3328 0.79 -1.7 3,459,399 - gspG b3328 0.81 +2.0 3,459,421 + gspG b3328 0.86 +1.2 3,459,446 - +1.1 3,459,460 - -0.4 3,459,460 - -4.3 3,459,519 - -2.7 3,459,519 - +0.6 3,459,530 - -2.1 3,459,530 - -0.3 3,459,540 - -1.1 3,459,570 + gspH b3329 0.16 -0.2 3,459,578 - gspH b3329 0.17 -1.5 3,459,603 - gspH b3329 0.22 +1.6 3,459,607 - gspH b3329 0.23 +0.5 3,459,607 - gspH b3329 0.23 -1.0 3,459,674 - gspH b3329 0.36 -1.4 3,459,698 - gspH b3329 0.41 +0.8 3,459,716 + gspH b3329 0.44 -0.1 3,459,742 + gspH b3329 0.49 -0.9 3,459,742 + gspH b3329 0.49 +1.3 3,459,747 + gspH b3329 0.50 -0.2 3,459,818 + gspH b3329 0.64 -0.8 3,459,851 - gspH b3329 0.71 +1.7 3,459,851 - gspH b3329 0.71 -0.4 3,459,893 + gspH b3329 0.79 +0.2 3,459,920 - gspH b3329 0.84 +0.4 3,459,930 + gspH b3329 0.86 +3.9 3,459,937 + gspH b3329 0.88 +0.5 3,459,937 + gspH b3329 0.88 -0.1 3,459,947 + gspH b3329 0.90 +0.4 3,459,947 + gspH b3329 0.90 -1.5 3,459,955 - -2.1 3,459,964 - -1.0 3,459,978 + -4.2 3,459,978 + -0.1 3,459,978 + +1.1 3,459,995 + +0.0 3,460,012 + -0.2 3,460,012 + -2.4 3,460,031 - +0.8 3,460,043 - yheH b3330 0.12 +0.4 3,460,057 + yheH b3330 0.16 +2.2 3,460,081 + yheH b3330 0.22 +0.1 3,460,081 + yheH b3330 0.22 -4.3 3,460,081 + yheH b3330 0.22 -0.2 3,460,081 + yheH b3330 0.22 -0.4 3,460,083 + yheH b3330 0.23 -3.1 3,460,083 + yheH b3330 0.23 +0.2 3,460,086 - yheH b3330 0.24 +1.0 3,460,086 - yheH b3330 0.24 -0.4 3,460,086 - yheH b3330 0.24 +0.5 3,460,089 - yheH b3330 0.25 -0.0 3,460,089 - yheH b3330 0.25 +1.1 3,460,107 + yheH b3330 0.29 +1.0 3,460,114 + yheH b3330 0.31 +2.3 3,460,133 + yheH b3330 0.36 +0.8 3,460,198 - yheH b3330 0.53 +1.1 3,460,198 - yheH b3330 0.53 +0.8 3,460,224 + yheH b3330 0.60 -0.7 3,460,248 - yheH b3330 0.67 -1.5 3,460,248 - yheH b3330 0.67 -0.7 3,460,248 - yheH b3330 0.67 -0.6 3,460,277 - yheH b3330 0.74 +0.6 3,460,280 + yheH b3330 0.75 +0.4 3,460,280 + yheH b3330 0.75 +0.0 3,460,280 + yheH b3330 0.75 +0.0 3,460,280 + yheH b3330 0.75 -0.5 3,460,280 + yheH b3330 0.75 -0.1 3,460,385 + +3.4 3,460,388 - +0.2 3,460,395 + +0.8 3,460,395 + -0.1 3,460,401 + +1.4 3,460,439 - gspJ b3331 0.12 -0.3 3,460,518 + -1.7 3,460,524 + gspJ b3331 0.27 -0.1 3,460,529 - gspJ b3331 0.28 -1.5 3,460,534 - gspJ b3331 0.29 -0.9 3,460,538 - gspJ b3331 0.29 +1.3 3,460,569 - gspJ b3331 0.35 -0.2 3,460,569 - gspJ b3331 0.35 -4.1 3,460,580 - gspJ b3331 0.36 -0.8 3,460,592 - gspJ b3331 0.38 -5.6 3,460,596 + gspJ b3331 0.39 +0.4 3,460,639 - gspJ b3331 0.46 +0.7 3,460,666 + -1.5 3,460,667 + gspJ b3331 0.51 -0.5 3,460,668 - gspJ b3331 0.51 +6.6 3,460,674 - gspJ b3331 0.52 +0.4 3,460,686 - gspJ b3331 0.54 +1.4 3,460,686 - gspJ b3331 0.54 +1.0 3,460,705 - gspJ b3331 0.58 -5.1 3,460,705 - gspJ b3331 0.58 +0.7 3,460,707 + gspJ b3331 0.58 +1.3 3,460,713 - gspJ b3331 0.59 -1.2 3,460,713 - gspJ b3331 0.59 +0.5 3,460,725 - gspJ b3331 0.61 +0.1 3,460,725 - gspJ b3331 0.61 +0.2 3,460,727 - gspJ b3331 0.61 +1.0 3,460,732 - gspJ b3331 0.62 -2.2 3,460,734 - gspJ b3331 0.63 +0.6 3,460,770 - gspJ b3331 0.69 +1.0 3,460,770 - gspJ b3331 0.69 +1.2 3,460,770 - gspJ b3331 0.69 +0.9 3,460,771 - gspJ b3331 0.69 +0.7 3,460,835 + gspJ b3331 0.80 -4.2 3,460,835 + gspJ b3331 0.80 +0.8 3,460,848 + gspJ b3331 0.82 -2.2 3,460,848 + gspJ b3331 0.82 +0.4 3,460,854 + gspJ b3331 0.83 +0.4 3,460,870 - gspJ b3331 0.86 +0.6 3,460,872 - gspJ b3331 0.86 +0.9 3,460,872 - gspJ b3331 0.86 +7.5 3,460,877 + gspJ b3331 0.87 +0.5 3,460,890 - gspJ b3331 0.89 +1.6 3,460,900 + -1.8 3,460,900 + +0.1 3,460,937 - +0.7 3,460,937 - -0.1 3,460,978 - -0.8 3,460,978 - +1.3 3,460,982 + -1.0 3,460,982 + -0.6 3,461,004 + -2.1 3,461,021 + +1.0 3,461,045 - gspK b3332 0.10 +1.5 3,461,045 - gspK b3332 0.10 +0.1 3,461,094 + gspK b3332 0.15 +1.7 3,461,129 - gspK b3332 0.19 -0.2 3,461,132 + gspK b3332 0.19 +0.5 3,461,139 - gspK b3332 0.20 +1.3 3,461,147 + gspK b3332 0.20 -1.2 3,461,176 + gspK b3332 0.23 +1.4 3,461,176 + gspK b3332 0.23 -0.6 3,461,176 + gspK b3332 0.23 +0.9 3,461,176 + gspK b3332 0.23 +1.2 3,461,178 + gspK b3332 0.24 +1.5 3,461,178 + gspK b3332 0.24 +0.1 3,461,184 - gspK b3332 0.24 +0.3 3,461,186 - gspK b3332 0.24 +0.7 3,461,186 - gspK b3332 0.24 -0.1 3,461,186 - gspK b3332 0.24 -1.0 3,461,201 + gspK b3332 0.26 +1.3 3,461,249 - gspK b3332 0.31 +1.5 3,461,253 + gspK b3332 0.31 +1.1 3,461,261 - gspK b3332 0.32 +0.8 3,461,289 + gspK b3332 0.35 -4.4 3,461,320 + gspK b3332 0.38 +0.5 3,461,323 + gspK b3332 0.38 +1.0 3,461,343 - gspK b3332 0.40 +0.7 3,461,343 - gspK b3332 0.40 -4.6 3,461,348 + gspK b3332 0.41 +1.6 3,461,348 + gspK b3332 0.41 +1.4 3,461,348 + gspK b3332 0.41 +1.8 3,461,352 - gspK b3332 0.41 +0.1
Or see this region's nucleotide sequence