Experiment: PBS_cage_2_mouse_1_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolB and pal are separated by 34 nucleotides pal and ybgF are separated by 9 nucleotides ybgF and lysT are separated by 164 nucleotides
b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255
tolB
b0741: pal - peptidoglycan-associated outer membrane lipoprotein (NCBI), at 778,290 to 778,811
pal
b0742: ybgF - hypothetical protein (NCBI), at 778,821 to 779,612
ybgF
b0743: lysT - tRNA-Lys (NCBI), at 779,777 to 779,852
lysT
Position (kb) 778
779 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 777.299 kb on - strand, within tolB at 777.303 kb on - strand, within tolB at 777.393 kb on - strand, within tolB at 777.398 kb on - strand, within tolB at 777.444 kb on - strand, within tolB at 777.513 kb on + strand, within tolB at 777.522 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.602 kb on - strand, within tolB at 777.692 kb on + strand, within tolB at 777.700 kb on - strand, within tolB at 777.720 kb on - strand, within tolB at 777.770 kb on - strand, within tolB at 777.792 kb on - strand, within tolB at 777.839 kb on - strand, within tolB at 777.952 kb on - strand, within tolB at 777.954 kb on - strand, within tolB at 777.987 kb on - strand, within tolB at 778.009 kb on - strand, within tolB at 778.014 kb on - strand, within tolB at 778.020 kb on - strand, within tolB at 778.070 kb on + strand, within tolB at 778.127 kb on - strand at 778.150 kb on - strand at 778.150 kb on - strand at 778.212 kb on + strand at 778.212 kb on + strand at 778.212 kb on + strand at 778.220 kb on - strand at 778.222 kb on + strand at 778.316 kb on + strand at 778.316 kb on + strand at 778.382 kb on + strand, within pal at 778.395 kb on - strand, within pal at 778.420 kb on - strand, within pal at 778.422 kb on - strand, within pal at 778.455 kb on + strand, within pal at 778.455 kb on + strand, within pal at 778.560 kb on + strand, within pal at 778.568 kb on - strand, within pal at 778.596 kb on - strand, within pal at 778.600 kb on + strand, within pal at 778.604 kb on - strand, within pal at 778.656 kb on - strand, within pal at 778.656 kb on - strand at 778.661 kb on - strand, within pal at 778.713 kb on - strand, within pal at 778.752 kb on + strand, within pal at 778.752 kb on + strand, within pal at 778.752 kb on + strand, within pal at 778.780 kb on + strand at 778.780 kb on + strand at 778.805 kb on - strand at 778.851 kb on + strand at 778.856 kb on + strand at 778.864 kb on - strand at 778.870 kb on + strand at 778.932 kb on + strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 778.940 kb on - strand, within ybgF at 779.036 kb on + strand, within ybgF at 779.145 kb on + strand, within ybgF at 779.145 kb on + strand, within ybgF at 779.158 kb on - strand, within ybgF at 779.196 kb on + strand, within ybgF at 779.201 kb on + strand, within ybgF at 779.201 kb on + strand, within ybgF at 779.204 kb on - strand, within ybgF at 779.263 kb on - strand, within ybgF at 779.263 kb on - strand, within ybgF at 779.269 kb on + strand, within ybgF at 779.342 kb on + strand, within ybgF at 779.342 kb on + strand, within ybgF at 779.392 kb on - strand, within ybgF at 779.428 kb on - strand, within ybgF at 779.510 kb on + strand, within ybgF at 779.544 kb on - strand at 779.544 kb on - strand at 779.565 kb on + strand at 779.610 kb on - strand at 779.619 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PBS_cage_2_mouse_1_Cecum_40 hr remove 777,299 - tolB b0740 0.26 -4.0 777,303 - tolB b0740 0.26 -2.9 777,393 - tolB b0740 0.33 -3.3 777,398 - tolB b0740 0.34 -0.7 777,444 - tolB b0740 0.37 -2.0 777,513 + tolB b0740 0.43 -3.2 777,522 - tolB b0740 0.43 -1.9 777,602 - tolB b0740 0.49 -2.0 777,602 - tolB b0740 0.49 -2.7 777,692 + tolB b0740 0.56 -2.6 777,700 - tolB b0740 0.57 +0.8 777,720 - tolB b0740 0.59 -3.7 777,770 - tolB b0740 0.62 -3.6 777,792 - tolB b0740 0.64 -2.9 777,839 - tolB b0740 0.68 -3.0 777,952 - tolB b0740 0.76 -3.0 777,954 - tolB b0740 0.77 -1.7 777,987 - tolB b0740 0.79 -3.3 778,009 - tolB b0740 0.81 -3.6 778,014 - tolB b0740 0.81 -2.9 778,020 - tolB b0740 0.82 -3.5 778,070 + tolB b0740 0.86 -3.3 778,127 - -3.3 778,150 - -3.6 778,150 - -3.4 778,212 + -2.5 778,212 + -0.9 778,212 + -1.0 778,220 - -2.9 778,222 + -3.8 778,316 + -4.0 778,316 + -4.2 778,382 + pal b0741 0.18 -3.5 778,395 - pal b0741 0.20 -0.9 778,420 - pal b0741 0.25 -2.2 778,422 - pal b0741 0.25 -3.6 778,455 + pal b0741 0.32 -2.2 778,455 + pal b0741 0.32 -3.4 778,560 + pal b0741 0.52 -4.1 778,568 - pal b0741 0.53 -3.7 778,596 - pal b0741 0.59 -3.9 778,600 + pal b0741 0.59 -2.7 778,604 - pal b0741 0.60 -3.1 778,656 - pal b0741 0.70 -1.9 778,656 - -2.6 778,661 - pal b0741 0.71 -4.5 778,713 - pal b0741 0.81 -3.2 778,752 + pal b0741 0.89 -3.4 778,752 + pal b0741 0.89 -2.0 778,752 + pal b0741 0.89 -4.6 778,780 + -3.9 778,780 + -0.7 778,805 - -5.2 778,851 + -1.0 778,856 + -1.0 778,864 - -1.8 778,870 + -1.1 778,932 + ybgF b0742 0.14 -2.1 778,940 - ybgF b0742 0.15 +0.2 778,940 - ybgF b0742 0.15 -4.3 778,940 - ybgF b0742 0.15 +1.9 778,940 - ybgF b0742 0.15 -3.3 779,036 + ybgF b0742 0.27 +0.9 779,145 + ybgF b0742 0.41 +0.8 779,145 + ybgF b0742 0.41 +1.0 779,158 - ybgF b0742 0.43 -0.9 779,196 + ybgF b0742 0.47 -1.3 779,201 + ybgF b0742 0.48 -4.1 779,201 + ybgF b0742 0.48 -4.4 779,204 - ybgF b0742 0.48 +0.8 779,263 - ybgF b0742 0.56 -3.1 779,263 - ybgF b0742 0.56 -5.0 779,269 + ybgF b0742 0.57 -1.1 779,342 + ybgF b0742 0.66 -3.0 779,342 + ybgF b0742 0.66 -5.2 779,392 - ybgF b0742 0.72 +0.1 779,428 - ybgF b0742 0.77 +0.1 779,510 + ybgF b0742 0.87 -3.7 779,544 - -3.7 779,544 - -1.7 779,565 + -0.2 779,610 - +0.8 779,619 + -0.3
Or see this region's nucleotide sequence