Experiment: PBS_cage_1_mouse_2_Cecum_40 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yghZ and yqhA are separated by 39 nucleotides yqhA and yghA are separated by 190 nucleotides yghA and exbD are separated by 271 nucleotides exbD and exbB are separated by 6 nucleotides
b3001: yghZ - aldo-keto reductase (NCBI), at 3,145,919 to 3,146,959
yghZ
b3002: yqhA - hypothetical protein (NCBI), at 3,146,999 to 3,147,493
yqhA
b3003: yghA - oxidoreductase (NCBI), at 3,147,684 to 3,148,568
yghA
b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265
exbD
b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006
exbB
Position (kb)
3147
3148
3149 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3146.753 kb on - strand, within yghZ at 3146.753 kb on - strand, within yghZ at 3146.753 kb on - strand, within yghZ at 3146.840 kb on + strand, within yghZ at 3146.842 kb on + strand, within yghZ at 3146.842 kb on + strand, within yghZ at 3146.863 kb on + strand at 3146.863 kb on + strand at 3146.869 kb on - strand at 3146.971 kb on - strand at 3146.995 kb on - strand at 3147.027 kb on + strand at 3147.309 kb on - strand, within yqhA at 3147.435 kb on - strand, within yqhA at 3147.435 kb on - strand, within yqhA at 3147.461 kb on + strand at 3147.526 kb on + strand at 3147.526 kb on + strand at 3147.531 kb on - strand at 3147.538 kb on + strand at 3147.538 kb on + strand at 3147.540 kb on + strand at 3147.540 kb on + strand at 3147.650 kb on - strand at 3147.713 kb on + strand at 3147.713 kb on + strand at 3147.713 kb on + strand at 3147.723 kb on + strand at 3147.783 kb on - strand, within yghA at 3147.865 kb on - strand, within yghA at 3147.865 kb on - strand, within yghA at 3147.981 kb on + strand, within yghA at 3147.993 kb on - strand, within yghA at 3147.993 kb on - strand, within yghA at 3148.064 kb on - strand, within yghA at 3148.064 kb on - strand, within yghA at 3148.078 kb on - strand, within yghA at 3148.175 kb on + strand, within yghA at 3148.175 kb on + strand, within yghA at 3148.175 kb on + strand, within yghA at 3148.175 kb on + strand, within yghA at 3148.183 kb on - strand, within yghA at 3148.183 kb on - strand, within yghA at 3148.226 kb on + strand, within yghA at 3148.250 kb on - strand, within yghA at 3148.259 kb on - strand, within yghA at 3148.282 kb on - strand, within yghA at 3148.282 kb on - strand, within yghA at 3148.299 kb on + strand, within yghA at 3148.299 kb on + strand, within yghA at 3148.371 kb on + strand, within yghA at 3148.384 kb on - strand, within yghA at 3148.384 kb on - strand, within yghA at 3148.386 kb on + strand, within yghA at 3148.386 kb on + strand, within yghA at 3148.387 kb on - strand, within yghA at 3148.387 kb on - strand, within yghA at 3148.515 kb on + strand at 3148.519 kb on + strand at 3148.540 kb on - strand at 3148.552 kb on - strand at 3148.611 kb on - strand at 3148.694 kb on + strand at 3148.700 kb on - strand at 3148.758 kb on - strand at 3148.758 kb on - strand at 3148.875 kb on - strand at 3148.906 kb on + strand, within exbD at 3148.936 kb on - strand, within exbD at 3148.992 kb on - strand, within exbD at 3149.036 kb on + strand, within exbD at 3149.092 kb on + strand, within exbD at 3149.120 kb on - strand at 3149.120 kb on - strand, within exbD at 3149.131 kb on + strand, within exbD at 3149.131 kb on + strand, within exbD at 3149.208 kb on + strand, within exbD at 3149.377 kb on + strand, within exbB at 3149.377 kb on + strand, within exbB at 3149.380 kb on + strand, within exbB at 3149.396 kb on - strand, within exbB at 3149.448 kb on - strand, within exbB at 3149.501 kb on + strand, within exbB at 3149.508 kb on + strand, within exbB at 3149.543 kb on - strand, within exbB at 3149.553 kb on - strand, within exbB at 3149.554 kb on + strand, within exbB at 3149.564 kb on + strand, within exbB
Per-strain Table
Position Strand Gene LocusTag Fraction PBS_cage_1_mouse_2_Cecum_40 hr remove 3,146,753 - yghZ b3001 0.80 +0.9 3,146,753 - yghZ b3001 0.80 +0.7 3,146,753 - yghZ b3001 0.80 -2.8 3,146,840 + yghZ b3001 0.88 +0.2 3,146,842 + yghZ b3001 0.89 -2.0 3,146,842 + yghZ b3001 0.89 -0.1 3,146,863 + -0.2 3,146,863 + +1.2 3,146,869 - -0.0 3,146,971 - +0.9 3,146,995 - +0.7 3,147,027 + -5.4 3,147,309 - yqhA b3002 0.63 -0.2 3,147,435 - yqhA b3002 0.88 -3.8 3,147,435 - yqhA b3002 0.88 -0.3 3,147,461 + +0.1 3,147,526 + +0.4 3,147,526 + +0.8 3,147,531 - -0.6 3,147,538 + +0.0 3,147,538 + +1.2 3,147,540 + -4.0 3,147,540 + -5.0 3,147,650 - -2.1 3,147,713 + -2.1 3,147,713 + +1.5 3,147,713 + -3.0 3,147,723 + -0.4 3,147,783 - yghA b3003 0.11 -0.8 3,147,865 - yghA b3003 0.20 -0.1 3,147,865 - yghA b3003 0.20 -0.5 3,147,981 + yghA b3003 0.34 +0.7 3,147,993 - yghA b3003 0.35 -5.6 3,147,993 - yghA b3003 0.35 +2.3 3,148,064 - yghA b3003 0.43 +1.6 3,148,064 - yghA b3003 0.43 +1.6 3,148,078 - yghA b3003 0.45 +2.4 3,148,175 + yghA b3003 0.55 -0.3 3,148,175 + yghA b3003 0.55 -4.4 3,148,175 + yghA b3003 0.55 +0.4 3,148,175 + yghA b3003 0.55 -1.1 3,148,183 - yghA b3003 0.56 +1.3 3,148,183 - yghA b3003 0.56 +0.4 3,148,226 + yghA b3003 0.61 +2.3 3,148,250 - yghA b3003 0.64 +1.4 3,148,259 - yghA b3003 0.65 -0.7 3,148,282 - yghA b3003 0.68 +0.5 3,148,282 - yghA b3003 0.68 +0.0 3,148,299 + yghA b3003 0.69 +0.4 3,148,299 + yghA b3003 0.69 +0.2 3,148,371 + yghA b3003 0.78 -1.4 3,148,384 - yghA b3003 0.79 +0.2 3,148,384 - yghA b3003 0.79 +1.4 3,148,386 + yghA b3003 0.79 -0.1 3,148,386 + yghA b3003 0.79 +1.8 3,148,387 - yghA b3003 0.79 +1.0 3,148,387 - yghA b3003 0.79 -0.4 3,148,515 + -0.2 3,148,519 + +0.6 3,148,540 - +0.7 3,148,552 - +0.1 3,148,611 - +0.3 3,148,694 + +0.9 3,148,700 - -2.3 3,148,758 - +0.3 3,148,758 - -0.6 3,148,875 - +0.4 3,148,906 + exbD b3005 0.15 +0.8 3,148,936 - exbD b3005 0.23 +0.5 3,148,992 - exbD b3005 0.36 +0.8 3,149,036 + exbD b3005 0.46 -4.1 3,149,092 + exbD b3005 0.59 -0.8 3,149,120 - -2.3 3,149,120 - exbD b3005 0.66 -3.0 3,149,131 + exbD b3005 0.68 +0.2 3,149,131 + exbD b3005 0.68 -0.2 3,149,208 + exbD b3005 0.86 +0.4 3,149,377 + exbB b3006 0.14 -4.8 3,149,377 + exbB b3006 0.14 +0.5 3,149,380 + exbB b3006 0.15 +1.1 3,149,396 - exbB b3006 0.17 +0.7 3,149,448 - exbB b3006 0.24 -0.9 3,149,501 + exbB b3006 0.31 -0.3 3,149,508 + exbB b3006 0.32 +0.4 3,149,543 - exbB b3006 0.37 -0.0 3,149,553 - exbB b3006 0.38 -2.0 3,149,554 + exbB b3006 0.38 +0.3 3,149,564 + exbB b3006 0.40 +1.5
Or see this region's nucleotide sequence