Strain Fitness in Escherichia coli BW25113 around b0347

Experiment: PBS_cage_1_mouse_1_Cecum_40 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmhpR and mhpA are separated by 190 nucleotidesmhpA and mhpB are separated by 1 nucleotidesmhpB and mhpC are separated by 2 nucleotides b0346: mhpR - DNA-binding transcriptional activator, 3HPP-binding (RefSeq), at 366,811 to 367,644 mhpR b0347: mhpA - 3-(3-hydroxyphenyl)propionate hydroxylase (NCBI), at 367,835 to 369,499 mhpA b0348: mhpB - 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI), at 369,501 to 370,445 mhpB b0349: mhpC - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS), at 370,448 to 371,329 mhpC Position (kb) 367 368 369 370Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 366.960 kb on + strand, within mhpRat 366.960 kb on + strand, within mhpRat 366.960 kb on + strand, within mhpRat 366.996 kb on + strand, within mhpRat 367.173 kb on - strand, within mhpRat 367.287 kb on + strand, within mhpRat 367.325 kb on + strand, within mhpRat 367.326 kb on - strand, within mhpRat 367.592 kb on - strandat 367.592 kb on - strandat 367.596 kb on + strandat 367.728 kb on + strandat 367.728 kb on + strandat 367.736 kb on - strandat 367.736 kb on - strandat 367.736 kb on - strandat 367.738 kb on - strandat 367.784 kb on + strandat 367.784 kb on + strandat 367.784 kb on + strandat 367.792 kb on - strandat 367.792 kb on - strandat 367.792 kb on - strandat 367.865 kb on + strandat 367.865 kb on + strandat 367.926 kb on - strandat 367.940 kb on - strandat 367.942 kb on + strandat 367.942 kb on + strandat 367.942 kb on + strandat 368.079 kb on - strand, within mhpAat 368.142 kb on - strand, within mhpAat 368.261 kb on - strand, within mhpAat 368.479 kb on + strand, within mhpAat 368.486 kb on - strand, within mhpAat 368.568 kb on - strand, within mhpAat 368.661 kb on - strand, within mhpAat 368.728 kb on + strand, within mhpAat 368.728 kb on + strand, within mhpAat 368.755 kb on + strand, within mhpAat 368.761 kb on - strand, within mhpAat 368.763 kb on + strand, within mhpAat 368.763 kb on + strand, within mhpAat 368.778 kb on + strand, within mhpAat 368.883 kb on - strand, within mhpAat 368.898 kb on - strand, within mhpAat 368.898 kb on - strand, within mhpAat 369.036 kb on - strand, within mhpAat 369.077 kb on - strand, within mhpAat 369.133 kb on + strand, within mhpAat 369.232 kb on + strand, within mhpAat 369.283 kb on + strand, within mhpAat 369.418 kb on + strandat 369.625 kb on + strand, within mhpBat 369.705 kb on - strand, within mhpBat 369.705 kb on - strand, within mhpBat 369.829 kb on + strand, within mhpBat 369.829 kb on + strand, within mhpBat 369.837 kb on - strand, within mhpBat 369.908 kb on + strand, within mhpBat 369.908 kb on + strand, within mhpBat 370.056 kb on - strand, within mhpBat 370.198 kb on - strand, within mhpBat 370.263 kb on + strand, within mhpBat 370.344 kb on + strand, within mhpBat 370.364 kb on + strandat 370.398 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PBS_cage_1_mouse_1_Cecum_40 hr
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366,960 + mhpR b0346 0.18 +0.1
366,960 + mhpR b0346 0.18 -3.4
366,960 + mhpR b0346 0.18 -0.3
366,996 + mhpR b0346 0.22 +2.4
367,173 - mhpR b0346 0.43 +1.1
367,287 + mhpR b0346 0.57 -2.5
367,325 + mhpR b0346 0.62 -4.8
367,326 - mhpR b0346 0.62 -1.0
367,592 - -1.2
367,592 - -0.1
367,596 + +0.6
367,728 + +1.1
367,728 + -2.4
367,736 - -0.8
367,736 - +0.6
367,736 - +0.7
367,738 - -0.3
367,784 + +2.1
367,784 + +0.4
367,784 + +0.4
367,792 - -4.0
367,792 - +1.0
367,792 - +0.1
367,865 + +1.6
367,865 + +1.3
367,926 - -0.2
367,940 - -0.1
367,942 + +3.2
367,942 + +2.1
367,942 + +2.1
368,079 - mhpA b0347 0.15 -0.6
368,142 - mhpA b0347 0.18 -4.1
368,261 - mhpA b0347 0.26 +0.3
368,479 + mhpA b0347 0.39 +0.7
368,486 - mhpA b0347 0.39 +3.9
368,568 - mhpA b0347 0.44 +3.4
368,661 - mhpA b0347 0.50 -3.3
368,728 + mhpA b0347 0.54 +1.8
368,728 + mhpA b0347 0.54 +1.4
368,755 + mhpA b0347 0.55 +0.1
368,761 - mhpA b0347 0.56 -0.0
368,763 + mhpA b0347 0.56 -1.2
368,763 + mhpA b0347 0.56 +3.2
368,778 + mhpA b0347 0.57 +2.2
368,883 - mhpA b0347 0.63 -0.4
368,898 - mhpA b0347 0.64 +0.3
368,898 - mhpA b0347 0.64 -4.5
369,036 - mhpA b0347 0.72 -1.3
369,077 - mhpA b0347 0.75 +2.7
369,133 + mhpA b0347 0.78 +0.1
369,232 + mhpA b0347 0.84 +0.7
369,283 + mhpA b0347 0.87 +1.1
369,418 + +2.1
369,625 + mhpB b0348 0.13 -1.5
369,705 - mhpB b0348 0.22 -3.0
369,705 - mhpB b0348 0.22 -2.5
369,829 + mhpB b0348 0.35 -5.3
369,829 + mhpB b0348 0.35 -4.1
369,837 - mhpB b0348 0.36 +1.1
369,908 + mhpB b0348 0.43 +2.9
369,908 + mhpB b0348 0.43 +2.5
370,056 - mhpB b0348 0.59 +0.9
370,198 - mhpB b0348 0.74 +1.1
370,263 + mhpB b0348 0.81 +0.8
370,344 + mhpB b0348 0.89 +0.4
370,364 + +0.9
370,398 - -0.9

Or see this region's nucleotide sequence