Strain Fitness in Pseudomonas putida KT2440 around PP_3226

Experiment: PUT8 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_3224 and PP_3225 are separated by 15 nucleotidesPP_3225 and PP_3226 overlap by 4 nucleotidesPP_3226 and PP_3227 are separated by 185 nucleotides PP_3224: PP_3224 - Class II aldolase/adducin domain protein, at 3,659,940 to 3,660,734 _3224 PP_3225: PP_3225 - D-isomer specific 2-hydroxyacid dehydrogenase family protein, at 3,660,750 to 3,661,694 _3225 PP_3226: PP_3226 - Acyl-CoA dehydrogenase-related protein, at 3,661,691 to 3,662,833 _3226 PP_3227: PP_3227 - Transcriptional regulator, LysR family, at 3,663,019 to 3,663,909 _3227 Position (kb) 3661 3662 3663Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3660.901 kb on + strand, within PP_3225at 3660.901 kb on + strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3661.052 kb on - strand, within PP_3225at 3661.161 kb on + strand, within PP_3225at 3661.339 kb on - strand, within PP_3225at 3661.564 kb on - strand, within PP_3225at 3661.775 kb on + strandat 3661.979 kb on + strand, within PP_3226at 3661.980 kb on - strand, within PP_3226at 3661.980 kb on - strand, within PP_3226at 3662.088 kb on + strand, within PP_3226at 3662.089 kb on - strand, within PP_3226at 3662.089 kb on - strand, within PP_3226at 3662.091 kb on - strand, within PP_3226at 3662.121 kb on - strand, within PP_3226at 3662.343 kb on + strand, within PP_3226at 3662.373 kb on + strand, within PP_3226at 3662.577 kb on + strand, within PP_3226at 3662.578 kb on - strand, within PP_3226at 3662.685 kb on - strand, within PP_3226at 3662.856 kb on + strandat 3662.901 kb on + strandat 3663.087 kb on - strandat 3663.136 kb on - strand, within PP_3227at 3663.142 kb on + strand, within PP_3227at 3663.143 kb on - strand, within PP_3227at 3663.143 kb on - strand, within PP_3227at 3663.176 kb on - strandat 3663.176 kb on - strand, within PP_3227at 3663.351 kb on - strandat 3663.368 kb on + strand, within PP_3227

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Per-strain Table

Position Strand Gene LocusTag Fraction PUT8 MOI 0.1
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3,660,901 + PP_3225 0.16 +1.4
3,660,901 + PP_3225 0.16 +0.2
3,660,902 - PP_3225 0.16 +0.2
3,660,902 - PP_3225 0.16 +0.3
3,660,902 - PP_3225 0.16 -1.3
3,661,052 - PP_3225 0.32 +0.1
3,661,161 + PP_3225 0.43 +0.2
3,661,339 - PP_3225 0.62 -0.3
3,661,564 - PP_3225 0.86 -0.4
3,661,775 + +2.3
3,661,979 + PP_3226 0.25 -1.2
3,661,980 - PP_3226 0.25 -0.1
3,661,980 - PP_3226 0.25 -4.4
3,662,088 + PP_3226 0.35 +0.2
3,662,089 - PP_3226 0.35 -1.1
3,662,089 - PP_3226 0.35 -1.7
3,662,091 - PP_3226 0.35 -3.7
3,662,121 - PP_3226 0.38 -0.2
3,662,343 + PP_3226 0.57 -0.7
3,662,373 + PP_3226 0.60 -0.5
3,662,577 + PP_3226 0.78 -1.7
3,662,578 - PP_3226 0.78 +1.2
3,662,685 - PP_3226 0.87 +0.5
3,662,856 + +1.4
3,662,901 + -0.4
3,663,087 - +0.3
3,663,136 - PP_3227 0.13 +0.8
3,663,142 + PP_3227 0.14 +0.6
3,663,143 - PP_3227 0.14 -0.6
3,663,143 - PP_3227 0.14 -1.3
3,663,176 - -1.5
3,663,176 - PP_3227 0.18 +0.4
3,663,351 - -1.4
3,663,368 + PP_3227 0.39 -1.2

Or see this region's nucleotide sequence