Experiment: PUT8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1320 and PP_1321 overlap by 20 nucleotides PP_1321 and PP_1322 are separated by 61 nucleotides PP_1322 and PP_1323 overlap by 4 nucleotides PP_1323 and PP_1324 are separated by 92 nucleotides
PP_1320: PP_1320 - stringent starvation protein A, at 1,506,996 to 1,507,619
_1320
PP_1321: PP_1321 - ClpXP protease specificity-enhancing factor, at 1,507,600 to 1,508,061
_1321
PP_1322: PP_1322 - putative Lipoprotein, at 1,508,123 to 1,508,701
_1322
PP_1323: PP_1323 - factor modulating bacterial replication archosome assembly, at 1,508,698 to 1,509,285
_1323
PP_1324: PP_1324 - putative endonuclease distantly related to archaeal Holliday junction resolvase, at 1,509,378 to 1,509,752
_1324
Position (kb)
1508
1509 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1507.149 kb on + strand, within PP_1320 at 1507.150 kb on - strand, within PP_1320 at 1507.150 kb on - strand, within PP_1320 at 1507.191 kb on + strand, within PP_1320 at 1507.191 kb on + strand, within PP_1320 at 1507.216 kb on - strand, within PP_1320 at 1507.216 kb on - strand, within PP_1320 at 1507.216 kb on - strand, within PP_1320 at 1507.256 kb on - strand, within PP_1320 at 1507.257 kb on + strand, within PP_1320 at 1507.346 kb on + strand, within PP_1320 at 1507.472 kb on - strand, within PP_1320 at 1507.480 kb on + strand, within PP_1320 at 1507.543 kb on - strand, within PP_1320 at 1507.543 kb on - strand, within PP_1320 at 1507.617 kb on + strand at 1507.618 kb on - strand at 1507.618 kb on - strand at 1507.649 kb on - strand, within PP_1321 at 1507.666 kb on + strand, within PP_1321 at 1507.667 kb on - strand, within PP_1321 at 1507.667 kb on - strand, within PP_1321 at 1507.701 kb on + strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.776 kb on + strand, within PP_1321 at 1507.776 kb on + strand, within PP_1321 at 1507.776 kb on + strand, within PP_1321 at 1507.777 kb on - strand, within PP_1321 at 1507.777 kb on - strand, within PP_1321 at 1507.894 kb on + strand, within PP_1321 at 1507.894 kb on + strand, within PP_1321 at 1507.894 kb on + strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.918 kb on - strand, within PP_1321 at 1508.059 kb on + strand at 1508.060 kb on - strand at 1508.133 kb on + strand at 1508.475 kb on + strand, within PP_1322 at 1508.476 kb on - strand, within PP_1322 at 1508.692 kb on + strand at 1509.303 kb on - strand at 1509.303 kb on - strand at 1509.303 kb on - strand at 1509.303 kb on - strand at 1509.351 kb on - strand at 1509.351 kb on - strand at 1509.355 kb on - strand at 1509.355 kb on - strand at 1509.355 kb on - strand at 1509.355 kb on - strand at 1509.355 kb on - strand at 1509.355 kb on - strand at 1509.467 kb on - strand, within PP_1324 at 1509.543 kb on - strand, within PP_1324 at 1509.598 kb on - strand, within PP_1324 at 1509.601 kb on - strand, within PP_1324 at 1509.601 kb on - strand, within PP_1324 at 1509.606 kb on - strand, within PP_1324 at 1509.692 kb on - strand, within PP_1324
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 0.1 remove 1,507,149 + PP_1320 0.25 +1.0 1,507,150 - PP_1320 0.25 +0.6 1,507,150 - PP_1320 0.25 +1.6 1,507,191 + PP_1320 0.31 +1.1 1,507,191 + PP_1320 0.31 +2.0 1,507,216 - PP_1320 0.35 +0.6 1,507,216 - PP_1320 0.35 +1.3 1,507,216 - PP_1320 0.35 -0.7 1,507,256 - PP_1320 0.42 +0.8 1,507,257 + PP_1320 0.42 +1.5 1,507,346 + PP_1320 0.56 +0.8 1,507,472 - PP_1320 0.76 +1.3 1,507,480 + PP_1320 0.78 +1.4 1,507,543 - PP_1320 0.88 +1.3 1,507,543 - PP_1320 0.88 +0.7 1,507,617 + +0.0 1,507,618 - -1.9 1,507,618 - +0.3 1,507,649 - PP_1321 0.11 +1.3 1,507,666 + PP_1321 0.14 -2.0 1,507,667 - PP_1321 0.15 -2.9 1,507,667 - PP_1321 0.15 -1.3 1,507,701 + PP_1321 0.22 +1.5 1,507,702 - PP_1321 0.22 +2.2 1,507,702 - PP_1321 0.22 -0.1 1,507,702 - PP_1321 0.22 -0.5 1,507,702 - PP_1321 0.22 +1.8 1,507,702 - PP_1321 0.22 +0.4 1,507,776 + PP_1321 0.38 +1.3 1,507,776 + PP_1321 0.38 -0.3 1,507,776 + PP_1321 0.38 -0.9 1,507,777 - PP_1321 0.38 +0.4 1,507,777 - PP_1321 0.38 +0.6 1,507,894 + PP_1321 0.64 -0.0 1,507,894 + PP_1321 0.64 -4.1 1,507,894 + PP_1321 0.64 -0.2 1,507,895 - PP_1321 0.64 +0.5 1,507,895 - PP_1321 0.64 -0.5 1,507,895 - PP_1321 0.64 +1.9 1,507,895 - PP_1321 0.64 +1.3 1,507,895 - PP_1321 0.64 +2.4 1,507,918 - PP_1321 0.69 +0.4 1,508,059 + +1.1 1,508,060 - +1.4 1,508,133 + -0.2 1,508,475 + PP_1322 0.61 +1.3 1,508,476 - PP_1322 0.61 +1.9 1,508,692 + +0.9 1,509,303 - +1.6 1,509,303 - +0.5 1,509,303 - +1.7 1,509,303 - +3.1 1,509,351 - +1.0 1,509,351 - +0.5 1,509,355 - +1.1 1,509,355 - +1.8 1,509,355 - +0.9 1,509,355 - +1.5 1,509,355 - +0.9 1,509,355 - +1.2 1,509,467 - PP_1324 0.24 +1.5 1,509,543 - PP_1324 0.44 +1.6 1,509,598 - PP_1324 0.59 +1.9 1,509,601 - PP_1324 0.59 +0.6 1,509,601 - PP_1324 0.59 +0.3 1,509,606 - PP_1324 0.61 -0.5 1,509,692 - PP_1324 0.84 +1.7
Or see this region's nucleotide sequence