Experiment: PUT8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0759 and PP_0760 are separated by 45 nucleotides PP_0760 and PP_0761 are separated by 268 nucleotides PP_0761 and PP_0762 are separated by 54 nucleotides
PP_0759: PP_0759 - conserved exported protein of unknown function, at 874,422 to 875,261
_0759
PP_0760: PP_0760 - conserved membrane protein of unknown function, at 875,307 to 875,891
_0760
PP_0761: PP_0761 - Ribosomal RNA small subunit methyltransferase C, at 876,160 to 877,158
_0761
PP_0762: PP_0762 - Glycerate dehydrogenase, at 877,213 to 878,178
_0762
Position (kb) 876
877
878 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 875.172 kb on - strand, within PP_0759 at 875.238 kb on + strand at 875.238 kb on + strand at 875.239 kb on - strand at 875.239 kb on - strand at 875.259 kb on + strand at 875.259 kb on + strand at 875.259 kb on + strand at 875.259 kb on + strand at 875.260 kb on - strand at 875.309 kb on - strand at 875.341 kb on + strand at 875.341 kb on + strand at 875.463 kb on - strand, within PP_0760 at 875.483 kb on + strand, within PP_0760 at 875.483 kb on + strand, within PP_0760 at 875.484 kb on - strand, within PP_0760 at 875.484 kb on - strand, within PP_0760 at 875.861 kb on + strand at 875.923 kb on + strand at 875.924 kb on - strand at 875.924 kb on - strand at 875.932 kb on + strand at 875.932 kb on + strand at 875.932 kb on + strand at 875.932 kb on + strand at 875.932 kb on + strand at 875.933 kb on - strand at 875.933 kb on - strand at 875.933 kb on - strand at 875.933 kb on - strand at 875.933 kb on - strand at 875.933 kb on - strand at 875.933 kb on - strand at 875.968 kb on + strand at 875.968 kb on + strand at 875.969 kb on - strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.058 kb on + strand at 876.059 kb on - strand at 876.059 kb on - strand at 876.059 kb on - strand at 876.059 kb on - strand at 876.059 kb on - strand at 876.071 kb on - strand at 876.100 kb on + strand at 876.101 kb on - strand at 876.101 kb on - strand at 876.129 kb on + strand at 876.179 kb on + strand at 876.179 kb on + strand at 876.180 kb on - strand at 876.338 kb on + strand, within PP_0761 at 876.346 kb on - strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.380 kb on - strand, within PP_0761 at 876.381 kb on + strand, within PP_0761 at 876.382 kb on - strand, within PP_0761 at 876.382 kb on - strand, within PP_0761 at 876.382 kb on - strand, within PP_0761 at 876.621 kb on + strand, within PP_0761 at 876.621 kb on + strand, within PP_0761 at 876.650 kb on - strand, within PP_0761 at 876.863 kb on + strand, within PP_0761 at 876.864 kb on - strand, within PP_0761 at 876.908 kb on + strand, within PP_0761 at 877.192 kb on + strand at 877.192 kb on + strand at 877.192 kb on + strand at 877.527 kb on + strand, within PP_0762 at 877.528 kb on - strand, within PP_0762 at 877.528 kb on - strand, within PP_0762 at 877.645 kb on + strand, within PP_0762 at 877.646 kb on - strand, within PP_0762 at 877.740 kb on + strand, within PP_0762 at 877.805 kb on + strand, within PP_0762 at 877.806 kb on - strand, within PP_0762 at 877.849 kb on - strand, within PP_0762 at 877.849 kb on - strand at 877.849 kb on - strand, within PP_0762
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 0.1 remove 875,172 - PP_0759 0.89 +0.1 875,238 + +0.6 875,238 + +0.4 875,239 - -0.6 875,239 - +0.3 875,259 + +0.4 875,259 + -0.7 875,259 + -0.1 875,259 + +0.2 875,260 - +0.4 875,309 - -4.3 875,341 + +1.6 875,341 + -0.6 875,463 - PP_0760 0.27 +1.2 875,483 + PP_0760 0.30 +1.1 875,483 + PP_0760 0.30 +0.4 875,484 - PP_0760 0.30 -1.4 875,484 - PP_0760 0.30 -0.6 875,861 + -1.3 875,923 + -0.0 875,924 - -0.6 875,924 - -0.2 875,932 + -1.0 875,932 + +0.1 875,932 + +1.0 875,932 + -2.3 875,932 + +0.0 875,933 - +0.3 875,933 - -0.3 875,933 - +0.6 875,933 - -3.1 875,933 - +0.5 875,933 - -1.0 875,933 - -0.4 875,968 + +0.6 875,968 + -2.1 875,969 - +0.8 876,058 + -0.1 876,058 + -0.1 876,058 + +0.8 876,058 + +1.4 876,058 + +1.6 876,058 + -1.0 876,058 + -0.4 876,058 + +2.4 876,059 - -0.5 876,059 - +1.0 876,059 - -3.5 876,059 - +0.3 876,059 - -0.3 876,071 - -1.6 876,100 + -2.9 876,101 - +1.2 876,101 - -2.4 876,129 + +3.0 876,179 + -1.2 876,179 + +0.6 876,180 - +0.2 876,338 + PP_0761 0.18 +1.6 876,346 - PP_0761 0.19 +0.8 876,379 + PP_0761 0.22 +1.4 876,379 + PP_0761 0.22 +1.9 876,379 + PP_0761 0.22 -0.4 876,379 + PP_0761 0.22 +0.8 876,380 - PP_0761 0.22 +2.1 876,381 + PP_0761 0.22 +1.5 876,382 - PP_0761 0.22 +0.9 876,382 - PP_0761 0.22 +1.4 876,382 - PP_0761 0.22 +0.6 876,621 + PP_0761 0.46 +1.3 876,621 + PP_0761 0.46 +1.4 876,650 - PP_0761 0.49 +1.6 876,863 + PP_0761 0.70 +1.7 876,864 - PP_0761 0.70 +1.6 876,908 + PP_0761 0.75 -2.9 877,192 + +1.4 877,192 + +0.2 877,192 + +1.7 877,527 + PP_0762 0.33 +0.3 877,528 - PP_0762 0.33 -0.6 877,528 - PP_0762 0.33 +0.3 877,645 + PP_0762 0.45 -2.6 877,646 - PP_0762 0.45 +0.8 877,740 + PP_0762 0.55 +1.0 877,805 + PP_0762 0.61 -1.3 877,806 - PP_0762 0.61 -2.9 877,849 - PP_0762 0.66 -0.0 877,849 - +0.9 877,849 - PP_0762 0.66 +0.3
Or see this region's nucleotide sequence