Experiment: PUT8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0500 and PP_0501 overlap by 8 nucleotides PP_0501 and PP_0502 are separated by 30 nucleotides
PP_0500: PP_0500 - dTDP-4-rhamnose reductase-related protein, at 589,877 to 590,761
_0500
PP_0501: PP_0501 - NAD-dependent epimerase/dehydratase family protein, at 590,754 to 591,686
_0501
PP_0502: PP_0502 - conserved protein of unknown function, at 591,717 to 592,160
_0502
Position (kb)
589
590
591 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 588.970 kb on + strand at 588.971 kb on - strand at 588.971 kb on - strand at 589.005 kb on + strand at 589.006 kb on - strand at 589.006 kb on - strand at 589.018 kb on - strand at 589.130 kb on + strand at 589.274 kb on - strand at 589.289 kb on + strand at 589.290 kb on - strand at 589.300 kb on + strand at 589.300 kb on + strand at 589.322 kb on - strand at 589.342 kb on + strand at 589.342 kb on + strand at 589.441 kb on + strand at 589.490 kb on + strand at 589.490 kb on + strand at 589.491 kb on - strand at 589.491 kb on - strand at 589.492 kb on + strand at 589.492 kb on + strand at 589.509 kb on - strand at 589.509 kb on - strand at 589.516 kb on + strand at 589.517 kb on - strand at 589.689 kb on + strand at 589.690 kb on - strand at 589.704 kb on + strand at 589.705 kb on - strand at 589.722 kb on + strand at 589.722 kb on + strand at 589.722 kb on + strand at 589.722 kb on + strand at 589.723 kb on - strand at 589.723 kb on - strand at 589.730 kb on - strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.760 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 0.1 remove 588,970 + +0.5 588,971 - +0.2 588,971 - +0.5 589,005 + -3.9 589,006 - +0.8 589,006 - +0.7 589,018 - +1.4 589,130 + -0.1 589,274 - +0.4 589,289 + +0.2 589,290 - +2.9 589,300 + -1.4 589,300 + -4.7 589,322 - -1.5 589,342 + -2.2 589,342 + -2.9 589,441 + -1.3 589,490 + -1.0 589,490 + -3.3 589,491 - +1.8 589,491 - -1.0 589,492 + -0.7 589,492 + -0.3 589,509 - +2.7 589,509 - +0.5 589,516 + -5.2 589,517 - +0.9 589,689 + -1.2 589,690 - +0.3 589,704 + -2.8 589,705 - -2.1 589,722 + +0.3 589,722 + +0.2 589,722 + -1.4 589,722 + -1.7 589,723 - +0.6 589,723 - +2.0 589,730 - -0.1 591,759 + +1.8 591,759 + +0.9 591,759 + +0.4 591,759 + +1.2 591,759 + -0.2 591,760 - -0.8 591,760 - +1.7 591,760 - -1.4 591,760 - +1.5
Or see this region's nucleotide sequence