Strain Fitness in Pseudomonas putida KT2440 around PP_0251

Experiment: PUT8 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0249 and PP_0250 overlap by 29 nucleotidesPP_0250 and PP_0251 are separated by 28 nucleotidesPP_0251 and PP_0252 are separated by 120 nucleotides PP_0249: PP_0249 - conserved protein of unknown function, at 304,149 to 304,769 _0249 PP_0250: PP_0250 - heat shock protein Hsp15 involved in ribosome recycling, at 304,741 to 305,142 _0250 PP_0251: PP_0251 - conserved membrane protein of unknown function, at 305,171 to 305,965 _0251 PP_0252: PP_0252 - 33 kDa chaperonin, at 306,086 to 306,985 _0252 Position (kb) 305 306Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 304.196 kb on + strandat 304.203 kb on + strandat 304.203 kb on + strandat 304.203 kb on + strandat 304.203 kb on + strandat 304.203 kb on + strandat 304.203 kb on + strandat 304.204 kb on - strandat 304.204 kb on - strandat 304.207 kb on + strandat 304.208 kb on - strandat 304.208 kb on - strandat 304.242 kb on + strand, within PP_0249at 304.665 kb on - strand, within PP_0249at 304.665 kb on - strand, within PP_0249at 304.665 kb on - strand, within PP_0249at 304.692 kb on + strand, within PP_0249at 304.692 kb on + strand, within PP_0249at 304.692 kb on + strand, within PP_0249at 304.693 kb on - strand, within PP_0249at 304.693 kb on - strand, within PP_0249at 304.693 kb on - strand, within PP_0249at 304.703 kb on - strand, within PP_0249at 304.703 kb on - strand, within PP_0249at 304.703 kb on - strand, within PP_0249at 304.703 kb on - strand, within PP_0249at 304.703 kb on - strand, within PP_0249at 304.705 kb on + strand, within PP_0249at 304.705 kb on + strand, within PP_0249at 304.705 kb on + strand, within PP_0249at 304.705 kb on + strand, within PP_0249at 304.705 kb on + strand, within PP_0249at 304.706 kb on - strand, within PP_0249at 304.706 kb on - strand, within PP_0249at 304.706 kb on - strand, within PP_0249at 304.706 kb on - strand, within PP_0249at 304.999 kb on + strand, within PP_0250at 305.052 kb on + strand, within PP_0250at 305.053 kb on - strand, within PP_0250at 305.204 kb on + strandat 305.284 kb on - strand, within PP_0251at 305.284 kb on - strand, within PP_0251at 305.284 kb on - strand, within PP_0251at 305.304 kb on - strand, within PP_0251at 305.328 kb on + strand, within PP_0251at 305.329 kb on - strand, within PP_0251at 305.406 kb on + strand, within PP_0251at 305.406 kb on + strand, within PP_0251at 305.407 kb on - strand, within PP_0251at 305.407 kb on - strand, within PP_0251at 305.407 kb on - strand, within PP_0251at 305.533 kb on - strand, within PP_0251at 305.535 kb on + strand, within PP_0251at 305.536 kb on - strand, within PP_0251at 305.536 kb on - strand, within PP_0251at 305.636 kb on + strand, within PP_0251at 305.636 kb on + strand, within PP_0251at 305.645 kb on + strand, within PP_0251at 305.645 kb on + strand, within PP_0251at 305.646 kb on - strand, within PP_0251at 305.759 kb on + strand, within PP_0251at 305.759 kb on + strand, within PP_0251at 305.760 kb on - strand, within PP_0251at 305.781 kb on + strand, within PP_0251at 305.781 kb on + strand, within PP_0251at 305.782 kb on - strand, within PP_0251at 305.874 kb on - strand, within PP_0251at 305.888 kb on + strandat 305.888 kb on + strandat 305.889 kb on - strandat 305.889 kb on - strandat 305.908 kb on + strandat 305.909 kb on - strandat 305.909 kb on - strandat 305.965 kb on + strandat 305.965 kb on + strandat 305.976 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PUT8 MOI 0.1
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304,196 + -0.1
304,203 + +0.5
304,203 + +0.3
304,203 + -1.4
304,203 + -2.1
304,203 + +1.2
304,203 + -0.2
304,204 - +0.1
304,204 - +0.7
304,207 + +0.0
304,208 - +1.3
304,208 - -0.3
304,242 + PP_0249 0.15 -0.5
304,665 - PP_0249 0.83 +1.0
304,665 - PP_0249 0.83 +0.1
304,665 - PP_0249 0.83 +1.9
304,692 + PP_0249 0.87 -0.8
304,692 + PP_0249 0.87 +0.2
304,692 + PP_0249 0.87 -2.0
304,693 - PP_0249 0.88 -0.3
304,693 - PP_0249 0.88 -3.6
304,693 - PP_0249 0.88 +0.1
304,703 - PP_0249 0.89 -2.1
304,703 - PP_0249 0.89 +0.4
304,703 - PP_0249 0.89 -3.1
304,703 - PP_0249 0.89 +0.7
304,703 - PP_0249 0.89 +0.5
304,705 + PP_0249 0.90 +1.3
304,705 + PP_0249 0.90 -0.2
304,705 + PP_0249 0.90 -0.7
304,705 + PP_0249 0.90 +1.3
304,705 + PP_0249 0.90 +0.1
304,706 - PP_0249 0.90 +0.4
304,706 - PP_0249 0.90 -1.3
304,706 - PP_0249 0.90 +1.9
304,706 - PP_0249 0.90 +1.0
304,999 + PP_0250 0.64 +0.6
305,052 + PP_0250 0.77 -0.7
305,053 - PP_0250 0.78 +0.7
305,204 + -1.6
305,284 - PP_0251 0.14 -1.6
305,284 - PP_0251 0.14 -3.2
305,284 - PP_0251 0.14 -0.0
305,304 - PP_0251 0.17 -2.8
305,328 + PP_0251 0.20 -1.9
305,329 - PP_0251 0.20 -0.9
305,406 + PP_0251 0.30 +0.4
305,406 + PP_0251 0.30 +1.1
305,407 - PP_0251 0.30 -3.6
305,407 - PP_0251 0.30 -3.2
305,407 - PP_0251 0.30 -4.5
305,533 - PP_0251 0.46 -4.4
305,535 + PP_0251 0.46 -1.3
305,536 - PP_0251 0.46 +0.9
305,536 - PP_0251 0.46 -3.3
305,636 + PP_0251 0.58 -2.1
305,636 + PP_0251 0.58 -3.1
305,645 + PP_0251 0.60 +2.6
305,645 + PP_0251 0.60 -0.6
305,646 - PP_0251 0.60 -1.3
305,759 + PP_0251 0.74 +0.4
305,759 + PP_0251 0.74 -2.8
305,760 - PP_0251 0.74 -0.7
305,781 + PP_0251 0.77 -0.3
305,781 + PP_0251 0.77 -0.1
305,782 - PP_0251 0.77 -1.2
305,874 - PP_0251 0.88 -0.6
305,888 + -0.3
305,888 + -1.6
305,889 - +1.0
305,889 - -3.0
305,908 + -1.1
305,909 - +0.7
305,909 - +0.2
305,965 + +0.3
305,965 + +0.7
305,976 + -1.4

Or see this region's nucleotide sequence