Experiment: PUT8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0185 and PP_0186 are separated by 118 nucleotides PP_0186 and PP_0187 overlap by 4 nucleotides PP_0187 and PP_0188 are separated by 18 nucleotides
PP_0185: PP_0185 - Positive alginate biosynthesis regulatory protein, at 237,120 to 237,863
_0185
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687
_0187
PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833
_0188
Position (kb) 237
238
239 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 237.076 kb on + strand at 237.076 kb on + strand at 237.076 kb on + strand at 237.077 kb on - strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.119 kb on + strand at 237.119 kb on + strand at 237.120 kb on - strand at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.196 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.540 kb on - strand, within PP_0185 at 237.594 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.781 kb on - strand, within PP_0185 at 237.902 kb on - strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.686 kb on - strand at 239.693 kb on + strand at 239.694 kb on - strand at 239.694 kb on - strand at 239.730 kb on + strand at 239.730 kb on + strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.762 kb on + strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.872 kb on + strand, within PP_0188 at 239.872 kb on + strand, within PP_0188 at 239.873 kb on - strand, within PP_0188
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 0.1 remove 237,076 + +0.0 237,076 + -3.8 237,076 + -0.9 237,077 - +1.0 237,081 + -1.8 237,081 + -0.0 237,081 + -4.7 237,082 - +1.0 237,082 - +0.8 237,082 - -1.7 237,083 + -0.5 237,083 + -3.5 237,083 + -0.2 237,083 + -3.3 237,084 - -0.9 237,084 - -1.3 237,084 - +0.7 237,084 - +1.0 237,084 - -1.2 237,119 + +1.0 237,119 + -1.0 237,120 - +0.7 237,195 + PP_0185 0.10 -1.7 237,195 + PP_0185 0.10 +0.1 237,195 + PP_0185 0.10 +0.3 237,196 - PP_0185 0.10 +0.7 237,379 - PP_0185 0.35 +0.5 237,379 - PP_0185 0.35 -0.1 237,379 - PP_0185 0.35 +0.7 237,540 - PP_0185 0.56 +0.4 237,594 + PP_0185 0.64 -0.5 237,615 + PP_0185 0.67 +0.3 237,615 + PP_0185 0.67 +1.9 237,615 + PP_0185 0.67 -0.1 237,615 + PP_0185 0.67 +1.1 237,615 + PP_0185 0.67 -1.7 237,615 + +2.1 237,615 + PP_0185 0.67 +0.5 237,615 + PP_0185 0.67 +0.7 237,615 + PP_0185 0.67 +1.2 237,616 - PP_0185 0.67 +0.4 237,616 - PP_0185 0.67 +0.8 237,616 - PP_0185 0.67 -0.7 237,616 - PP_0185 0.67 +0.1 237,616 - PP_0185 0.67 +1.0 237,781 - PP_0185 0.89 +1.4 237,902 - +1.3 239,685 + -1.7 239,685 + -1.2 239,685 + -0.1 239,685 + -3.8 239,685 + -1.5 239,686 - -0.2 239,693 + +1.0 239,694 - +0.9 239,694 - -2.8 239,730 + +0.8 239,730 + +0.1 239,731 - -0.1 239,731 - +0.3 239,731 - +0.9 239,762 + +0.1 239,763 - +0.8 239,763 - +0.7 239,763 - +2.8 239,872 + PP_0188 0.15 -0.8 239,872 + PP_0188 0.15 -3.7 239,873 - PP_0188 0.15 +0.4
Or see this region's nucleotide sequence