Experiment: PUT8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2142 and PP_2143 are separated by 13 nucleotides PP_2143 and PP_2144 are separated by 215 nucleotides PP_2144 and PP_2145 are separated by 177 nucleotides
PP_2142: PP_2142 - Cell division inhibitor SulA, at 2,444,413 to 2,444,889
_2142
PP_2143: PP_2143 - transcriptional repressor, at 2,444,903 to 2,445,511
_2143
PP_2144: PP_2144 - Transcriptional regulator, TetR family, at 2,445,727 to 2,446,434
_2144
PP_2145: PP_2145 - beta-N-acetylglucosaminidase, at 2,446,612 to 2,447,622
_2145
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2445.156 kb on - strand, within PP_2143 at 2445.169 kb on - strand, within PP_2143 at 2445.549 kb on - strand at 2445.682 kb on + strand at 2445.713 kb on + strand at 2445.723 kb on - strand at 2445.819 kb on + strand, within PP_2144 at 2445.819 kb on + strand, within PP_2144 at 2446.005 kb on - strand, within PP_2144 at 2446.005 kb on - strand, within PP_2144 at 2446.109 kb on - strand, within PP_2144 at 2446.123 kb on + strand, within PP_2144 at 2446.123 kb on + strand, within PP_2144 at 2446.123 kb on + strand at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.124 kb on - strand, within PP_2144 at 2446.144 kb on + strand, within PP_2144 at 2446.523 kb on + strand at 2446.524 kb on - strand at 2446.524 kb on - strand at 2446.657 kb on - strand at 2446.657 kb on - strand at 2446.732 kb on - strand, within PP_2145 at 2446.826 kb on + strand, within PP_2145 at 2446.827 kb on - strand, within PP_2145 at 2447.257 kb on + strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.258 kb on - strand, within PP_2145 at 2447.434 kb on - strand, within PP_2145
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 0.1 remove 2,445,156 - PP_2143 0.42 +1.9 2,445,169 - PP_2143 0.44 +2.2 2,445,549 - +1.0 2,445,682 + +2.6 2,445,713 + +0.2 2,445,723 - +2.0 2,445,819 + PP_2144 0.13 +1.1 2,445,819 + PP_2144 0.13 +0.4 2,446,005 - PP_2144 0.39 +2.6 2,446,005 - PP_2144 0.39 +4.4 2,446,109 - PP_2144 0.54 +0.2 2,446,123 + PP_2144 0.56 +1.2 2,446,123 + PP_2144 0.56 +1.5 2,446,123 + +1.6 2,446,124 - PP_2144 0.56 +0.7 2,446,124 - PP_2144 0.56 -1.0 2,446,124 - PP_2144 0.56 +3.5 2,446,124 - PP_2144 0.56 +4.3 2,446,124 - PP_2144 0.56 +2.2 2,446,144 + PP_2144 0.59 +0.2 2,446,523 + -0.2 2,446,524 - -3.2 2,446,524 - -3.5 2,446,657 - -3.6 2,446,657 - -3.5 2,446,732 - PP_2145 0.12 -2.4 2,446,826 + PP_2145 0.21 -3.6 2,446,827 - PP_2145 0.21 -3.4 2,447,257 + PP_2145 0.64 -1.9 2,447,258 - PP_2145 0.64 -3.7 2,447,258 - PP_2145 0.64 -3.7 2,447,258 - PP_2145 0.64 -3.7 2,447,258 - PP_2145 0.64 -3.0 2,447,434 - PP_2145 0.81 -4.9
Or see this region's nucleotide sequence