Experiment: PUT8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0858 and PP_0859 overlap by 13 nucleotides PP_0859 and PP_0860 are separated by 124 nucleotides PP_0860 and PP_0861 are separated by 231 nucleotides
PP_0858: PP_0858 - putative methionine/glutamine aminotransferase, at 992,994 to 994,142
_0858
PP_0859: PP_0859 - putative ketoglutaramate omega-amidase, at 994,130 to 994,921
_0859
PP_0860: PP_0860 - putative Sulfite reductase, flavoprotein component, at 995,046 to 997,595
_0860
PP_0861: PP_0861 - outer membrane ferric siderophore receptor, at 997,827 to 1,000,118
_0861
Position (kb) 995
996
997
998 Strain fitness (log2 ratio) -2
-1
0
1
2 at 994.076 kb on - strand at 994.129 kb on + strand at 994.145 kb on - strand at 994.145 kb on - strand at 994.211 kb on + strand, within PP_0859 at 994.211 kb on + strand, within PP_0859 at 994.280 kb on - strand, within PP_0859 at 994.338 kb on - strand, within PP_0859 at 994.466 kb on + strand, within PP_0859 at 994.467 kb on - strand, within PP_0859 at 994.467 kb on - strand, within PP_0859 at 994.511 kb on + strand, within PP_0859 at 994.511 kb on + strand, within PP_0859 at 994.578 kb on - strand, within PP_0859 at 994.578 kb on - strand, within PP_0859 at 994.634 kb on + strand, within PP_0859 at 994.635 kb on - strand, within PP_0859 at 994.712 kb on + strand, within PP_0859 at 994.712 kb on + strand, within PP_0859 at 994.713 kb on - strand, within PP_0859 at 994.760 kb on + strand, within PP_0859 at 994.760 kb on + strand, within PP_0859 at 994.761 kb on - strand, within PP_0859 at 994.843 kb on - strand at 994.843 kb on - strand at 994.903 kb on + strand at 994.904 kb on - strand at 995.036 kb on + strand at 995.036 kb on + strand at 995.050 kb on + strand at 995.050 kb on + strand at 995.050 kb on + strand at 995.050 kb on + strand at 995.050 kb on + strand at 995.051 kb on - strand at 995.051 kb on - strand at 995.091 kb on + strand at 995.092 kb on - strand at 995.167 kb on + strand at 995.167 kb on + strand at 995.167 kb on + strand at 995.168 kb on - strand at 995.236 kb on + strand at 995.236 kb on + strand at 995.236 kb on + strand at 995.236 kb on + strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.237 kb on - strand at 995.487 kb on - strand, within PP_0860 at 995.487 kb on - strand, within PP_0860 at 995.838 kb on + strand, within PP_0860 at 995.839 kb on - strand, within PP_0860 at 995.899 kb on + strand, within PP_0860 at 995.900 kb on - strand, within PP_0860 at 996.172 kb on + strand, within PP_0860 at 996.172 kb on + strand, within PP_0860 at 996.172 kb on + strand, within PP_0860 at 996.172 kb on + strand, within PP_0860 at 996.173 kb on - strand, within PP_0860 at 996.500 kb on - strand, within PP_0860 at 996.580 kb on + strand, within PP_0860 at 996.602 kb on - strand, within PP_0860 at 996.602 kb on - strand, within PP_0860 at 996.615 kb on + strand, within PP_0860 at 996.616 kb on - strand, within PP_0860 at 996.616 kb on - strand, within PP_0860 at 996.643 kb on + strand, within PP_0860 at 996.644 kb on - strand, within PP_0860 at 996.779 kb on - strand, within PP_0860 at 996.779 kb on - strand, within PP_0860 at 996.970 kb on + strand, within PP_0860 at 996.971 kb on - strand, within PP_0860 at 996.971 kb on - strand, within PP_0860 at 996.971 kb on - strand, within PP_0860 at 997.007 kb on - strand, within PP_0860 at 997.007 kb on - strand, within PP_0860 at 997.007 kb on - strand, within PP_0860 at 997.194 kb on - strand, within PP_0860 at 997.291 kb on + strand, within PP_0860 at 997.292 kb on - strand, within PP_0860 at 997.292 kb on - strand, within PP_0860 at 997.464 kb on + strand at 997.464 kb on + strand at 997.464 kb on + strand at 997.464 kb on + strand at 997.464 kb on + strand at 997.465 kb on - strand at 997.465 kb on - strand at 997.507 kb on + strand at 997.508 kb on - strand at 997.508 kb on - strand at 997.642 kb on + strand at 997.642 kb on + strand at 997.642 kb on + strand at 997.643 kb on - strand at 997.643 kb on - strand at 997.643 kb on - strand at 997.643 kb on - strand at 997.732 kb on + strand at 997.733 kb on - strand at 997.906 kb on + strand at 997.909 kb on + strand at 997.910 kb on - strand at 997.910 kb on - strand at 997.969 kb on + strand at 997.969 kb on + strand at 997.988 kb on - strand at 997.992 kb on + strand at 997.996 kb on + strand at 997.996 kb on + strand at 997.996 kb on + strand at 997.996 kb on + strand at 997.996 kb on + strand at 997.997 kb on - strand at 997.997 kb on - strand at 997.997 kb on - strand at 997.997 kb on - strand at 998.032 kb on + strand at 998.032 kb on + strand at 998.033 kb on - strand at 998.090 kb on - strand, within PP_0861 at 998.191 kb on + strand, within PP_0861 at 998.191 kb on + strand, within PP_0861 at 998.191 kb on + strand, within PP_0861 at 998.191 kb on + strand, within PP_0861 at 998.192 kb on - strand, within PP_0861 at 998.192 kb on - strand, within PP_0861 at 998.192 kb on - strand, within PP_0861 at 998.192 kb on - strand, within PP_0861 at 998.192 kb on - strand, within PP_0861 at 998.192 kb on - strand, within PP_0861 at 998.192 kb on - strand, within PP_0861 at 998.197 kb on + strand, within PP_0861 at 998.197 kb on + strand, within PP_0861 at 998.197 kb on + strand, within PP_0861 at 998.198 kb on - strand, within PP_0861 at 998.284 kb on + strand, within PP_0861 at 998.284 kb on + strand, within PP_0861 at 998.284 kb on + strand, within PP_0861 at 998.284 kb on + strand, within PP_0861 at 998.288 kb on + strand, within PP_0861 at 998.289 kb on - strand, within PP_0861 at 998.289 kb on - strand, within PP_0861 at 998.289 kb on - strand, within PP_0861 at 998.393 kb on - strand, within PP_0861 at 998.477 kb on + strand, within PP_0861 at 998.478 kb on - strand, within PP_0861 at 998.521 kb on + strand, within PP_0861 at 998.521 kb on + strand, within PP_0861 at 998.521 kb on + strand, within PP_0861 at 998.522 kb on - strand, within PP_0861 at 998.522 kb on - strand, within PP_0861 at 998.530 kb on + strand, within PP_0861 at 998.530 kb on + strand at 998.530 kb on + strand, within PP_0861 at 998.530 kb on + strand, within PP_0861 at 998.530 kb on + strand, within PP_0861 at 998.530 kb on + strand, within PP_0861 at 998.530 kb on + strand, within PP_0861 at 998.531 kb on - strand, within PP_0861 at 998.531 kb on - strand, within PP_0861 at 998.531 kb on - strand, within PP_0861 at 998.531 kb on - strand, within PP_0861 at 998.531 kb on - strand, within PP_0861
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 0.1 remove 994,076 - +0.2 994,129 + -0.8 994,145 - +1.5 994,145 - -0.6 994,211 + PP_0859 0.10 -0.4 994,211 + PP_0859 0.10 -0.6 994,280 - PP_0859 0.19 +1.8 994,338 - PP_0859 0.26 -0.7 994,466 + PP_0859 0.42 +0.0 994,467 - PP_0859 0.43 +0.3 994,467 - PP_0859 0.43 -1.0 994,511 + PP_0859 0.48 +0.9 994,511 + PP_0859 0.48 +0.5 994,578 - PP_0859 0.57 +1.0 994,578 - PP_0859 0.57 -1.3 994,634 + PP_0859 0.64 +1.0 994,635 - PP_0859 0.64 +1.0 994,712 + PP_0859 0.73 +0.6 994,712 + PP_0859 0.73 -0.8 994,713 - PP_0859 0.74 +0.8 994,760 + PP_0859 0.80 +0.6 994,760 + PP_0859 0.80 -0.5 994,761 - PP_0859 0.80 -1.4 994,843 - -0.9 994,843 - +0.1 994,903 + -1.2 994,904 - -0.8 995,036 + +0.6 995,036 + +1.6 995,050 + +1.1 995,050 + +1.2 995,050 + -0.5 995,050 + +1.4 995,050 + -0.2 995,051 - +0.4 995,051 - -0.3 995,091 + -0.2 995,092 - +0.3 995,167 + -1.0 995,167 + -0.4 995,167 + +0.6 995,168 - +2.2 995,236 + -0.7 995,236 + -0.2 995,236 + -0.2 995,236 + -0.5 995,237 - +0.8 995,237 - -0.7 995,237 - -1.0 995,237 - -1.0 995,237 - +0.6 995,237 - -2.4 995,237 - +0.5 995,237 - -0.2 995,237 - +0.1 995,237 - +0.0 995,487 - PP_0860 0.17 +0.3 995,487 - PP_0860 0.17 -0.0 995,838 + PP_0860 0.31 +0.9 995,839 - PP_0860 0.31 +0.1 995,899 + PP_0860 0.33 -0.7 995,900 - PP_0860 0.33 -0.3 996,172 + PP_0860 0.44 +0.7 996,172 + PP_0860 0.44 -0.2 996,172 + PP_0860 0.44 -0.6 996,172 + PP_0860 0.44 -0.2 996,173 - PP_0860 0.44 -0.3 996,500 - PP_0860 0.57 +0.1 996,580 + PP_0860 0.60 -0.2 996,602 - PP_0860 0.61 -0.2 996,602 - PP_0860 0.61 -0.3 996,615 + PP_0860 0.62 -1.4 996,616 - PP_0860 0.62 -2.7 996,616 - PP_0860 0.62 -1.3 996,643 + PP_0860 0.63 -0.5 996,644 - PP_0860 0.63 -0.3 996,779 - PP_0860 0.68 +0.5 996,779 - PP_0860 0.68 -1.1 996,970 + PP_0860 0.75 +0.4 996,971 - PP_0860 0.75 -0.3 996,971 - PP_0860 0.75 -0.3 996,971 - PP_0860 0.75 -1.0 997,007 - PP_0860 0.77 -2.3 997,007 - PP_0860 0.77 -0.9 997,007 - PP_0860 0.77 -2.0 997,194 - PP_0860 0.84 -1.1 997,291 + PP_0860 0.88 -0.8 997,292 - PP_0860 0.88 +0.6 997,292 - PP_0860 0.88 -2.1 997,464 + +0.2 997,464 + +2.4 997,464 + -0.4 997,464 + -1.3 997,464 + -0.5 997,465 - -0.1 997,465 - -0.4 997,507 + -1.1 997,508 - -0.7 997,508 - -0.0 997,642 + -0.5 997,642 + -0.0 997,642 + -0.6 997,643 - -0.3 997,643 - +0.0 997,643 - +0.0 997,643 - -0.7 997,732 + +0.3 997,733 - +0.2 997,906 + +0.4 997,909 + -1.7 997,910 - +0.5 997,910 - +0.6 997,969 + -1.9 997,969 + +0.5 997,988 - -0.7 997,992 + +0.3 997,996 + +0.5 997,996 + +0.5 997,996 + +0.3 997,996 + +0.3 997,996 + -0.3 997,997 - +1.3 997,997 - -0.8 997,997 - +0.2 997,997 - +0.2 998,032 + +0.9 998,032 + +0.4 998,033 - -0.1 998,090 - PP_0861 0.11 +1.2 998,191 + PP_0861 0.16 +0.6 998,191 + PP_0861 0.16 -0.2 998,191 + PP_0861 0.16 +1.3 998,191 + PP_0861 0.16 -0.4 998,192 - PP_0861 0.16 +1.1 998,192 - PP_0861 0.16 -1.2 998,192 - PP_0861 0.16 +0.3 998,192 - PP_0861 0.16 +0.4 998,192 - PP_0861 0.16 -0.7 998,192 - PP_0861 0.16 -0.7 998,192 - PP_0861 0.16 -1.9 998,197 + PP_0861 0.16 -1.0 998,197 + PP_0861 0.16 +1.6 998,197 + PP_0861 0.16 +0.6 998,198 - PP_0861 0.16 +0.3 998,284 + PP_0861 0.20 -0.8 998,284 + PP_0861 0.20 +1.0 998,284 + PP_0861 0.20 -0.5 998,284 + PP_0861 0.20 +0.1 998,288 + PP_0861 0.20 +0.3 998,289 - PP_0861 0.20 -1.0 998,289 - PP_0861 0.20 -0.0 998,289 - PP_0861 0.20 -0.1 998,393 - PP_0861 0.25 -0.2 998,477 + PP_0861 0.28 +0.4 998,478 - PP_0861 0.28 +0.8 998,521 + PP_0861 0.30 -1.5 998,521 + PP_0861 0.30 +0.5 998,521 + PP_0861 0.30 -0.6 998,522 - PP_0861 0.30 +0.2 998,522 - PP_0861 0.30 +0.7 998,530 + PP_0861 0.31 -0.5 998,530 + -1.5 998,530 + PP_0861 0.31 +0.8 998,530 + PP_0861 0.31 -0.6 998,530 + PP_0861 0.31 +0.7 998,530 + PP_0861 0.31 -0.3 998,530 + PP_0861 0.31 -0.8 998,531 - PP_0861 0.31 -1.3 998,531 - PP_0861 0.31 +0.6 998,531 - PP_0861 0.31 +1.1 998,531 - PP_0861 0.31 +0.7 998,531 - PP_0861 0.31 +0.5
Or see this region's nucleotide sequence